[1]赵志平,李健,王云超,等.LncRNA LOC100506123对胰腺癌细胞增殖及迁移的影响[J].第三军医大学学报,2017,39(09):840-845.
 Zhao Zhiping,Li Jian,Wang Yunchao,et al.Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells[J].J Third Mil Med Univ,2017,39(09):840-845.
点击复制

LncRNA LOC100506123对胰腺癌细胞增殖及迁移的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第09期
页码:
840-845
栏目:
基础医学
出版日期:
2017-05-15

文章信息/Info

Title:
Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells
作者:
赵志平李健王云超秦欢高维武王晨辉王槐志
第三军医大学:西南医院全军肝胆外科研究所,基础医学部全军免疫研究所
Author(s):
Zhao Zhiping Li Jian Wang Yunchao Qin Huan Gao Weiwu Wang Chenhui Wang Huaizhi

Institute of Hepatobiliary Surgery, Southwest Hospital, Institute of Immunology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China

关键词:
胰腺癌增殖迁移长链非编码RNARNA干扰
Keywords:
pancreatic cancer proliferation migration long non-coding RNA RNA interference
分类号:
R394.2; R730.23; R735.9
文献标志码:
A
摘要:

目的     探讨长链非编码RNA(long non-coding RNA,LncRNA)LOC100506123对胰腺癌细胞增殖及迁移的影响。方法     采用实时定量PCR (qRT-PCR)技术检测胰腺癌组织及细胞系(AsPC-1、BxPC-3、Panc-1、CFPAC-1,以正常胰腺导管上皮细胞HPDE为对照)中LOC100506123的表达情况。并通过siRNA技术下调LOC100506123表达,构建siRNA经Lipofectamine 2000TM脂质体转染细胞(对照组为siRNC,实验组为siR-1、siR-2),采用CCK-8、Transwell小室实验分别检测胰腺癌细胞增殖和转移能力。结果     ①LncRNA LOC100506123在胰腺癌组织及细胞系中表达显著升高(P<0.05)。②下调LOC100506123表达,胰腺癌细胞的增殖及迁移能力显著降低(P<0.05)。结论    高表达的LncRNA LOC100506123能促进胰腺癌细胞的增殖及迁移

Abstract:

Objective      To determine the effect of long non-coding RNA (LncRNA) LOC100506123 on the proliferation and migration of pancreatic cancer cells. Methods      Quantitative real-time PCR (qRTPCR) was used to detect the expression of LOC100506123 in pancreatic cell lines, including normal pancreatic duct epithelial cell line HPDE and 4 pancreatic cancer cell lines, AsPC-1, BxPC-3, Panc-1 and CFPAC-1, and pancreatic cancer tissues. Small interfering RNA (siRNA) targeting LOC100506123 by RNA interference technique was constructed  and then transfected into AsPC-1 and CFPAC-1 cells with Lipofectamine 2000 (including the control group siR-NC and the experimental groups siR-1 and siR-2). The proliferation and migration of pancreatic cancer cells were detected by Cell Counting Kit-8 (CCK-8) assay and Transwell chamber test, respectively. Results      ①LncRNA LOC100506123 was over-expressed in the pancreatic cancer cells and tissues (P<0.05). ②Downregulation of LOC100506123 significantly inhibited the proliferation and migration of pancreatic cancer cells (P<0.05). Conclusion      Over-expressed LOC100506123 promotes the proliferation and migration in pancreatic cancer cells.

参考文献/References:

[1]Abel E V, Simeone D M. Biology and clinical applications of pancreatic cancer stem cells[J]. Gastroenterology, 2013, 144(6): 1241-1248. DOI:10.1053/j.gastro.2013.01.072.
[2]Chen W, Zheng R, Baade P D, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132. DOI:10.3322/caac.21338.
[3]Hijiya N, Tsukamoto Y, Nakada C, et al. Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma[J]. Cancer Res, 2016, 76(9): 2612-2625. DOI:10.1158/0008-5472. CAN-15-1846.
[4]Xu Y, Chang R, Peng Z, et al. Loss of polarity protein AF6 promotes pancreatic cancer metastasis by inducing Snail expression[J]. Nat Commun, 2015, 6: 7184. DOI:10.1038/ncomms8184.
[5]Li Y, Chen H, Pan T, et al. LncRNA ontology: inferring lncRNA functions based on chromatin states and expression patterns[J]. Oncotarget, 2015, 6(37): 39793-39805. DOI:10.18632/oncotarget.5794.
[6]Silva A, Bullock M, Calin G. The Clinical Relevance of Long Non-Coding RNAs in Cancer[J]. Cancers, 2015, 7(4): 2169-2182. DOI:10.3390/cancers7040884.
[7]Li J, Liu D, Hua R, et al. Long non-coding RNAs expressed in pancreatic ductal adenocarcinoma and lncRNA BC008363 an independent prognostic factor in PDAC[J]. Pancreatology, 2014, 14(5): 385-390. DOI:10.1016/j.pan.2014.07.013.
[8]Di Gesualdo F, Capaccioli S, Lulli M. A pathophysiological view of the long non-coding RNA world[J]. Oncotarget, 2014, 5(22): 10976-10996. DOI:10.18632/oncotarget.2770.
[9]He Y, Meng X M, Huang C, et al. Long noncoding RNAs: Novel insights into hepatocelluar carcinoma[J]. Cancer Lett, 2014, 344(1): 20-27. DOI:10.1016/j.canlet.2013.10.021.
[10]Cheng Y, Jutooru I, Chadalapaka G, et al. The long non-coding RNA HOTTIP enhances pancreatic cancer cell proliferation, survival and migration[J]. Oncotarget, 2015, 6(13): 10840-10852. DOI:10.18632/oncotarget.3450.
[11]Lu Z, Xiao Z, Liu F, et al. Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1)[J]. Oncotarget, 2016, 7(1): 241-254. DOI:10.18632/oncotarget.6280.
[12]Ji J, Tang J, Deng L, et al. LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway[J]. Oncotarget, 2015, 6(40): 42813-42824. DOI:10.18632/oncotarget.5970.
[13]郭勤浩, 赵岩, 陈洁静, 等. BRAF激活的长链非编码RNA在结直肠癌中的表达及功能[J]. 中华消化外科杂志, 2014, 13(5): 376-380. DOI:10.3760/cma.j.issn. 1673-9752.2014.05.011.
Guo Q H, Zhao Y, Chen J J, et al. Expression and function of BRAF-activated long non-coding RNA in colorectal cancer[J]. Chin J Dig Surg,2014, 13(5): 376-380. DOI:10.3760/cma.j.issn.16739752.2014.05.011.
[14]Zhao L, Guo H, Zhou B, et al. Long non-coding RNA SNHG5 suppresses gastric cancer progression by trapping MTA2 in the cytosol[J]. Oncogene, 2016, 35(44): 5770-5780. DOI:10.1038/onc.2016.110.
[15]Matsumura K, Kawasaki Y, Miyamoto M, et al. The novel G-quadruplex-containing long noncoding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration[J]. Oncogene, 2017,36(9):1191-1199. DOI:10.1038/onc.2016.282.
[16]Vennin C, Spruyt N, Robin Y M, et al. The long non-coding RNA 91H increases aggressive phenotype of breast cancer cells and up-regulates H19/IGF2 expression through epigenetic modifications[J]. Cancer Lett, 2017, 385: 198-206. DOI:10.1016/j.canlet.2016.10.023.
[17]吕细林, 黄刚, 肖曼, 等. 过表达长链非编码RNA H19促进miR-124-3p表达抑制肝癌细胞增殖[J]. 第三军医大学学报, 2016, 38(6): 554-559. DOI:10.16016/j. 1000-5404.201508089.
Lyu X L, Huang G, Xiao M, et al. LncRNA H19-mediated miR-124-3p up-regulation suppresses proliferation in hepatocellular carcinoma cells[J]. J Third Mil Med Univ, 2016,38(6):554-559. DOI:10.16016/j.1000-5404.201508089.
[18]Wang Y, Li Z, Zheng S, et al. Expression profile of long non-coding RNAs in pancreatic cancer and their clinical significance as biomarkers[J]. Oncotarget, 2015, 6(34): 35684-35698. DOI:10.18632/oncotarget.5533.
[19]Li X, Deng S J, Zhu S, et al. Hypoxia-induced lncRNA-NUTF2P3-001 contributes to tumorigenesis of pancreatic cancer by derepressing the miR-3923/KRAS pathway[J]. Oncotarget, 2016, 7(5): 6000-6014. DOI:10.18632/oncotarget.6830.
[20]Deer E L, Gonz lez-Hern ndez J, Coursen J D, et al. Phenotype and genotype of pancreatic cancer cell lines[J]. Pancreas, 2010, 39(4): 425-435. DOI:10.1097/MPA. 0b013e3181c15963.

相似文献/References:

[1]李雪梅,罗淑娟,车亚玲,等.Rap1酶激动剂C3G对Ⅰ型卵巢癌细胞增殖和侵袭的影响[J].第三军医大学学报,2015,37(17):1715.
 Li Xuemei,Luo Shujuan,Che Yaling,et al.Effect of Rap1 activator C3G on proliferation and invasion in type Ⅰ ovarian cancer cells[J].J Third Mil Med Univ,2015,37(09):1715.
[2]朱宁生,张婧,曾晓华,等.BMP-4基因影响乳腺癌细胞增殖、凋亡和迁移能力涉及NF-κB途径[J].第三军医大学学报,2012,34(18):1910.
[3]黄士隋,史良会,黄广岩.RNA干扰下调LAT1表达对胃腺癌SGC-7901细胞增殖、侵袭、转移及细胞周期的影响[J].第三军医大学学报,2013,35(04):320.
 Huang Shisui,Shi Lianghui,Huang Guangyan.Effect of RNA interference targeting LAT1 on proliferation, migration and invasion of SGC7901 cells[J].J Third Mil Med Univ,2013,35(09):320.
[4]杨其芬,邵秉一,杨德琴,等.miR-181a对骨质疏松小鼠骨髓贴壁基质细胞体外增殖活性的影响[J].第三军医大学学报,2015,37(19 ):1966.
 Yang Qifen,Shao Bingyi,Yang Deqin,et al.Effect of miR-181a on proliferation of bone marrow mesenchymal stem cells from mouse osteoporosis model[J].J Third Mil Med Univ,2015,37(09):1966.
[5]李维东,罗浩军,李振华,等.雌激素激活GPER-EGFR-ERK通路促进人乳腺癌SKBR-3细胞系增殖[J].第三军医大学学报,2012,34(22):2283.
 Li Weidong,Luo Haojun,Li Zhenhua,et al.Estrogen activates GPER-EGFR-ERK pathway to promote the proliferation of human breast cancer cell line SKBR-3[J].J Third Mil Med Univ,2012,34(09):2283.
[6]吴登艳,宋娇,董海良,等.mTOR信号通路介导黄芩苷抑制人结肠癌细胞的增殖[J].第三军医大学学报,2012,34(23):2399.
 Wu Dengyan,Song Jiao,Dong Hailiang,et al.mTOR signaling pathway mediates baicalin-inhibited proliferation in human colon cancer cell line HCT116[J].J Third Mil Med Univ,2012,34(09):2399.
[7]赖明广,姚君,王立生.HSP90抑制剂17-DMAG调控胰腺癌细胞PANC-1增殖及凋亡的初步研究[J].第三军医大学学报,2013,35(07):627.
 Lai Mingguang,Yao Jun,Wang Lisheng.Heat shock protein 90 inhibitor, 17-DMAG, suppresses proliferation and induces apoptosis in pancreatic cancer cells in vitro[J].J Third Mil Med Univ,2013,35(09):627.
[8]张晓辉,孙乃学,冯朝晖,等.ROCK-Ⅰ选择性抑制剂Y-27632对人Tenon囊成纤维细胞增殖、凋亡和黏附性的影响[J].第三军医大学学报,2013,35(01):38.
 Zhang Xiaohui,Sun Naixue,Feng Zhaohui,et al.Effect of Y-27632, a selective inhibitor of ROCK-I, on proliferation, apoptosis and adhesion of human ocular Tenon’s capsular fibroblasts[J].J Third Mil Med Univ,2013,35(09):38.
[9]李明,周永,糜漫天.染料木黄酮对人乳腺癌细胞株MDA-MB-453、MCF-7、MCF-7/HER2增殖能力的影响[J].第三军医大学学报,2008,30(17):1618.
 LI Ming,ZHOU Yong,MI Man-tian.Effect of genistein on proliferation of human breast cancer cell lines MDA-MB-453, MCF-7 and MCF-7/HER2[J].J Third Mil Med Univ,2008,30(09):1618.
[10]江永红,黄晶,邓昌明,等.吲哚-3-甲醇对大鼠动脉损伤后内膜增殖的影响[J].第三军医大学学报,2007,29(15):1504.
 JIANG Yong-hong,HUANG Jing,DENG Chang-ming,et al.Effects of indole-3-carbinol on neointimal hyperplasia of rat artery after balloon injury[J].J Third Mil Med Univ,2007,29(09):1504.

更新日期/Last Update: 2017-05-05