[1]徐倩,杨佳佳,刘志华,等.低氧和牙周炎对牙周组织中HIF-1α和MMP2表达的影响[J].第三军医大学学报,2017,39(07):616-621.
 Xu Qian,Yang Jiajia,Liu Zhihua,et al.Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium[J].J Third Mil Med Univ,2017,39(07):616-621.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第07期
页码:
616-621
栏目:
基础医学
出版日期:
2017-04-15

文章信息/Info

Title:
Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium
作者:
徐倩杨佳佳刘志华刘锐安建平周霞陈发明邓蔓菁
第三军医大学大坪医院野战外科研究所口腔科;第四军医大学口腔医学院牙周科
Author(s):
Xu Qian Yang Jiajia Liu Zhihua Liu Rui An Jianping Zhou Xia Chen Faming Deng Manjing

Department of Stomatology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042; Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi’an, Shaanxi Province, 710032, China

关键词:
HIF-1&alphaMMP2牙周炎低氧
分类号:
R362; R780.2; R781.42
文献标志码:
A
摘要:

目的     研究低氧条件下HIF-1α、MMP2在大鼠牙周炎牙周组织中的表达及其与牙周炎的相关性,初步探讨HIF-1α与MMP2之间的关系。方法     建立低氧牙周炎动物模型,40只大鼠采用简单随机化分组法分为常氧组、常氧牙周炎组、低氧组、低氧牙周炎组,每组10只,分别检测牙龈指数、菌斑指数、探诊深度及牙槽骨吸收度4项牙周炎临床指标;牙周组织切片HE染色观察牙周炎症程度;免疫组化及Western blot检测牙周组织中HIF-1α、MMP2的表达。结果     牙周炎各临床指标及组织切片的HE染色均表明低氧牙周炎组大鼠牙周组织炎症程度最重;与常氧两组比较,低氧组均促进了牙周组织中HIF-1α、MMP2的表达(P<0.05);与非牙周炎组比较,牙周炎组HIF-1α、MMP2表达明显增加(P<0.05);低氧牙周炎组HIF-1α、MMP2表达最高(P<0.05)。结论     低氧促进了牙周炎组织中HIF-1α、MMP2的表达,加速了牙周炎的发展进程。

Abstract:

Objective      To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and matrix metalloproteinase 2 (MMP2) in the periodontal tissues of rats with periodontitis under hypoxia, and explore the relationship of HIF-1α and MMP2 expression with periodontitis. Methods      Forty healthy adult rats were randomly divided into 4 groups: normoxia group, normoxic periodontitis group, hypoxia group (simulating 5 000 m above sea level) and hypoxic periodontitis group (n=10 for each group). Gingival index, plaque index, depth of probing and alveolar bone resorption were measured respectively. HE staining was used to observe periodontal inflammation in the periodontal tissue sections. Immunohistochemical assay and Western blotting were employed to detect the expression levels of HIF-1α and MMP2 in the periodontal tissues. Results      Clinical indicators of periodontitis and the results of HE staining showed that the periodontal inflammation of the hypoxic periodontitis group was the most severe. Compared with the 2 normoxic groups, hypoxia promoted the expression of HIF-1α and MMP2 in the periodontal tissues in the 2 hypoxic groups (P<0.05). The expression of HIF-1α and MMP2 was significantly higher in periodontitis groups than in the non-periodontitis groups (P<0.05), with the highest levels in the hypoxic periodontitis group (P<0.05). Conclusion      Hypoxia promotes the expression levels of HIF-1α and MMP2 in periodontitis and accelerates its progression.

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更新日期/Last Update: 2017-04-07