[1]贺纯静,叶生丽,邹晋峰,等.颈交感神经阻滞对糖尿病大鼠脑线粒体功能的影响[J].第三军医大学学报,2017,39(13):1371-1375.
 HE Chunjing,YE Shengli,ZOU Jinfeng,et al.Effect of cervical sympathetic ganglia block on hippocampal mitochondria in diabetic rats[J].J Third Mil Med Univ,2017,39(13):1371-1375.
点击复制

颈交感神经阻滞对糖尿病大鼠脑线粒体功能的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第13期
页码:
1371-1375
栏目:
基础医学
出版日期:
2017-07-15

文章信息/Info

Title:
Effect of cervical sympathetic ganglia block on hippocampal mitochondria in diabetic rats
作者:
贺纯静叶生丽邹晋峰康新国农佳元代宏
贵州省人民医院:疼痛科,神经内科
Author(s):
HE Chunjing YE Shengli ZOU Jinfeng KANG Xinguo NONG Jiayuan DAI Hong

Department of Pain Management, Department of Neurology,  Guizhou Provincial People’s Hospital, Guiyang, Guizhou Province, 550002, China

关键词:
交感神经阻滞糖尿病线粒体水迷宫实验
Keywords:
sympathetic ganglia block diabetics mellitus mitochondria water maze test
分类号:
R329.25; R614.4; R587.1
文献标志码:
A
摘要:

目的       观察颈交感神经阻滞对糖尿病大鼠脑线粒体功能的影响。方法        雄性成年大鼠40只随机分为3组:对照组(n=10,腹腔注射注射等量缓冲液),糖尿病组(n=15,采用STZ糖尿病模型按65 mg/kg剂量腹腔1次注射1%STZ制备糖尿病大鼠),糖尿病+颈交感神经阻滞组(n=15,按65 mg/kg 剂量腹腔1次注射1%STZ,糖尿病成模后8周给予颈交感神经阻滞治疗)。造模后12周水迷宫实验观察各组大鼠的认知功能。并于12周处死大鼠,采用Clark氧电极法测定大鼠海马线粒体的呼吸功能,免疫组化检测海马IL-6、TNF-α的表达。结果         造模后12周,糖尿病组存活12只,阻滞组存活13只。糖尿病组、阻滞组与对照组相比,逃避潜伏期明显延长、穿越平台的次数明显减少(P<0.05)。糖尿病组线粒体呼吸功能为R3(28.51±3.01)、R4( 13.93±1.16)、RCR(2.09±0.27),阻滞组的为R3 (45.26±4.21)、R4( 11.99±1.11)、RCR( 3.52±0.32),对照组为R3(66.31±5.65)、R4(12.97±3.38)、RCR (4.78±0.68),糖尿病组和阻滞组线粒体呼吸功能均较对照组组明显下降,主要表现为R3和RCR值显著降低(P<0.05);但阻滞组的R3和RCR高于糖尿病组(P<0.05)。在海马CA1 区,糖尿病组IL-6 、TNF-α表达为(53.32±6.29)、(63.21±13.24),阻滞组为 (28.14±3.82)、(32.76±5.98),均高于对照组(13.87±2.54)、(11.09±8.87)(P<0.05)。阻滞组明显低于糖尿病组,差异有统计学意义(P<0.05)。 结论         颈交感神经阻滞可改善糖尿病大鼠脑线粒体功能,其机制可能与其下调IL-6 、TNF-α有关。

Abstract:

Objective         To determine the effect of cervical sympathetic ganglia block on  hippocampal mitochondria respiratory function in diabetic rats.  Methods       A total of 40 male adult rats were randomly divided into 3 groups: control (n=10), diabetes (n=15), and diabetes+cervical sympathetic ganglia block group (n=15, block group). After the rat model of diabetes were established by intraperitoneal injection of 1% STZ at a dose of 65 mg/kg, and cervical sympathetic ganglia block was carried out in 8 weeks after model establishment. Water maze test was used to observe the cognitive function in rats in 12 weeks after model establishment. Then the rats were sacrificed at 12 weeks, and the hippocampal mitochondria respiratory function was observed by Clark oxygen electrode, and the expression levels of IL-6 and TNF-α were determined by immunohistochemical assay. Results           At 12 weeks after model establishment, there were 12 mice in the diabetes group and 13 in the block group. The escape latency was significantly extended and the number of crossing the platform was obviously reduced in diabetic group and cervical group when compared with control group (P<0.05). Respiratory state 3 (R3), R4, and respiratory control rate (RCR) were 45.26±4.21, 11.99±1.11 and 3.52±0.32, respectively in the diabetic group, and were 66.31±5.65, 12.97±3.38 and 4.78±0.68, respectively in the control group. The mitochondrial respiratory function were significantly lower in the diabetic group and block group than the control group, mainly with significantly decreased R3 and RCR (P<0.05). But R3 and RCR were significantly higher in the block group than the diabetic group (P<0.05). In the CA1 region of the hippocampus, the expression levels of IL-6 and TNF-α were 53.32±6.29 and 63.21±13.24 in the diabetic group,  and 28.14±3.82 and 32.76±5.98 in the block group, and the levels were obviously higher in the both groups than the control group (13.87±2.54, 11.09±8.87, P<0.05). But the levels in the block group was significantly lower than those in the diabetic group (P<0.05).  Conclusion         Cervical sympathetic ganglia improves brain mitochondrial function in diabetic rats, which may be associated with its down-regulating IL-6 and TNF-α.

参考文献/References:

[1]RAMOS-RODRIGNEZ J J,ORTIZ O,JIMENEZPALOMARES M,et al.Differential central pathology and  cognitive impairment  in  prediabetic and  diabetic mice[J].Psychoneuroendocrinology,2013,38(11):2462-2475.DOI: 10.1016/j.psyneuen.2013.05.010.
[2]SONNEN J A,LN E B,BRICKELL K,et al.Different patterns of cerebral injury in dementia with or without  diabetes[J]. Arch Neurol, 2009, 66(3):315-322.DOI: 10.1001/archneurol.2008.579.
[3] 庄丽英,张志瑁.神经炎症与阿尔茨海默病[J].中华行为医学与脑科学杂志,2012,21(7):664-665. DOI:10.3760/cma.j.issn.16746554.2012.07.030.
ZHUANG L Y, ZHANG Z M. Nerve inflammation and Alzheimer’s disease[J].  Chin J Behav Med Brain Sci, 2012,21(7):664-665. DOI:10.3760/cma.j.issn.1674-6554.2012.07.030.
[4]SONNEN J A, LARSON E B, BRICKELL K, et al. Different patterns of cerebral injury in dementia with or without diabetes[J]. Arch Neurol, 2009, 66(3):315-322. DOI 10.1001/archneurol.2008.579.
[5]REDDY P H, REDDY T P. Mitochondria as a therapeutic target for aging and neurodegenerative diseases[J]. Curr Alzheimer Res, 2011, 8(4): 393-409.
[6]DINEL A L, ANDRE C, AUBERT A, et al. Cognitive and emotional alterations are related to hippocampal inflammation in a mouse model of metabolic syndrome[J]. Plos One,2011,6(9):e24325. DOI: 10.1371/journal.pone.0024325.
[7]YU N, WANG S, WANG P, et al. The calcium uniporter regulates the permeability transition pore in isolated cortical mitochondria[J]. Neural Regen Res, 2012, 7(2): 109-113. DOI: 10.3969/j.issn.1673-5374.2012.02.005.
[8]吴晶,张明强,贾敏, 等. 线粒体抗氧化肽对脓毒性脑病小鼠认知功能和线粒体功能的影响[J]. 临床麻醉学杂志,2014, 8(30):788-791.
WU J, ZHANG M Q, JIA M, et al. Effects of mitochondrial-targeted peptide on cognitive and mitochondrial functions in a mouse model of sepsis-associated encephalopathy[J]. J Clin Anesthesiol, 2014, 8(30):788-791.
[9]SARIOGˇLU Y, UTKAN T, AKG-N M, et al. Effects of deferoxamine and sympathectomy on endothelin-1-induced contraction and acetylcholine-induced relaxation following subarachnoid hemorrhage in carotid artery[J]. Gen Pharmacol, 1997, 28(1):145-151. DOI:10.1016/S03063623(96)001541.
[10]TREGGIARI M M, ROMAND J A, MARTIN J B, et al. Cervical sympathetic block to reverse delayed ischemic neurological deficits after aneurismal subarachnoid hemorrhage[J]. Stroke, 2003, 34(4):961-967.  DOI:org/10.1161/01.STR.0000060893.72098.80
[11]HE C J, LIU G D, NIE H X, et al. Effect of cervical sympathetic block on cerebral vasospasm after subarachnoid hemorrhage in rabbits[J]. Acta Cir Bra, 2013, 28(1):1-11. DOI.org/10.1590/S0102-86502013000200001
[12]LIU G D, HE C J. Stellate ganglion block promotes recovery of Bell’s palsy in patients with diabetes mellitus[J]. Acta Otolaryngol, 2014,134(6):652-655.  DOI: 10.3109/00016489.2014.880794
[13]ZEINSTRA E, WILCZAK N, DE KEYSER J. [3H]dihydroalprenolol binding to  beta adrenergic receptors in multiple sclerosis brain[J].Neurosci Lett, 2000, 289(1):75-77.DOI.org/10.1016/S0304-3940(00)01254-4
[14]MORIN D, SAPENA R, ZINI R, et al. Characterization of betaadrenergic receptors of freshly  isolated astrocytes and neurons from rat brain[J].Life Sci,1997, 60(4-5): 315-324.
[15]RAINS J L, JAIN S K. Oxidative stress, insulin signaling, and diabetes[J]. Free Radic Biol Med,2011,50(5)567-575. DOI: 10.1016/j.freeradbiomed.2010.12.006

相似文献/References:

[1]牟海刚,古平.莱姆病伴直立性低血压误诊为糖尿病心血管自主神经病1例[J].第三军医大学学报,2012,34(15):1542.
[2]周厚地,童强,段炼,等.胃大部切除术致糖尿病患者反复低血糖1例[J].第三军医大学学报,2012,34(17):1753.
[3]吴艳,王毅,曾绍凡,等.老年2型糖尿病瘦素、骨密度变化及相关因素的研究[J].第三军医大学学报,2007,29(22):2188.
 WU Yan,WANG Yi,ZENG Shao-fan,et al.Bone density, leptin level and other related factors in elder patients with type 2 diabetes[J].J Third Mil Med Univ,2007,29(13):2188.
[4]李志勤.饮食护理干预对老年糖尿病患者血糖和糖化血红蛋白水平的影响[J].第三军医大学学报,2012,34(20):2130.
[5]徐灵莉,邓本敏,唐玲,等.护理风险管理能有效提高糖尿病合并恶性肿瘤患者的化疗疗效[J].第三军医大学学报,2013,35(04):351.
 Xu Lingli,Deng Benmin,Tang Ling,et al.Risk management of nursing improves chemotherapy efficiency for diabetic patients with malignant tumor[J].J Third Mil Med Univ,2013,35(13):351.
[6]魏平.糖尿病治疗的新趋势及相关热点追踪[J].第三军医大学学报,2012,34(23):2435.
[7]李明秀,王建,樊荣,等.癌症合并糖尿病患者住院期间的营养治疗[J].第三军医大学学报,2007,29(21):2079.
[8]高原,肖谦,赵柯湘,等.替米沙坦对糖尿病大鼠认知功能的保护作用[J].第三军医大学学报,2007,29(15):1514.
 GAO Yuan,XIAO Qian,ZHAO Ke-Xiang,et al.The study of the protection of telmisartan on the cognitive function in diabetic rats[J].J Third Mil Med Univ,2007,29(13):1514.
[9]范伟,杨妤欣,陈隽,等.糖尿病大鼠早期骨微结构改变及其BMP-2基因的表达[J].第三军医大学学报,2007,29(11):1053.
 FAN Wei,YANG Yu-xin,CHEN Juan,et al.Bone microstructure changes and bone BMP-2 expression in rats at early period of diabetes[J].J Third Mil Med Univ,2007,29(13):1053.
[10]杨光玉,卿尚兰,陈英.糖尿病患者根管治疗中疼痛有关因素的临床研究[J].第三军医大学学报,2007,29(10):982.
 YANG Guang-yu,QING Shang-lan,CHEN Ying.Correlation of diabetes mellitus and endodontic interappointment pain[J].J Third Mil Med Univ,2007,29(13):982.

更新日期/Last Update: 2017-07-14