[1]吴念,张平,田冬冬,等.ATRA与BMP9对人骨肉瘤细胞增殖及分化作用的研究[J].第三军医大学学报,2017,39(13):1333-1338.
 WU Nian,ZHANG Ping,TIAN Dongdong,et al.Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells[J].J Third Mil Med Univ,2017,39(13):1333-1338.
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ATRA与BMP9对人骨肉瘤细胞增殖及分化作用的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第13期
页码:
1333-1338
栏目:
基础医学
出版日期:
2017-07-15

文章信息/Info

Title:
Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells
作者:
吴念张平田冬冬张然熙陈前昭何百成尹良军
重庆医科大学附属第二医院骨科;重庆市生物化学与分子药理学重点实验室;重庆医科大学药学院药理学教研室
Author(s):
WU Nian ZHANG Ping TIAN Dongdong ZHANG Ranxi CHEN Qianzhao HE Baicheng YIN Liangjun

Department of Orthopaedics, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010; Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing, 400016; Department of Pharmacology, Pharmacy School of Chongqing Medical University, Chongqing, 400016, China

关键词:
骨肉瘤全反式维甲酸骨形态蛋白9增殖成骨分化
Keywords:
osteosarcoma all-trans retinoic acid bone morphogenetic protein 9 proliferation osteogenic differentiation
分类号:
R34; R730.23; R738.1
文献标志码:
A
摘要:

目的          研究全反式维甲酸(all-trans-retinoic acid,ATRA)与骨形态发生蛋白9(bone morphogenetic proteins 9,BMP9)对人骨肉瘤细胞增殖及分化的影响。方法         利用四甲基偶氮唑蓝(MTT)法和流式细胞仪分析细胞增殖情况;蛋白质免疫印迹实验(Western blot)检测细胞增殖和成骨相关指标;半定量PCR及Western blot分析ATRA和ATRA与BMP9联用处理143B细胞株后对BMP9表达水平的影响。结果         在人骨肉瘤143B细胞株中,与对照组相比,ATRA能明显抑制细胞增殖(P<0.05),并促使细胞周期阻滞在G1期(P<0.05),下调PCNA的蛋白水平,并呈浓度依赖性;ATRA能呈浓度依赖性增加晚期成骨指标OCN和OPN的蛋白水平(P<0.05);ATRA能上调BMP9的mRNA及蛋白水平,并呈浓度依赖性(P<0.05);单用BMP9并不能上调OCN和OPN表达,并促进其增殖(P<0.05),但与ATRA联用时,能够增强ATRA诱导的相关成骨指标表达和抗增殖作用(P<0.05)。 结论        ATRA对143B细胞的增殖具有抑制作用并诱导其成骨分化,并可能恢复BMP9的成骨诱导能力。

Abstract:

Objective        To determine the effects of all-trans-retinoic acid (ATRA) and bone morphogenetic protein 9 (BMP9) on the proliferation and osteogenic differentiation of human osteosarcoma (OS) cells. Methods         MTT assay and flow cytometry were used to analyze the proliferation of the human OS cell line 143B. Western blot analysis was used to detect the proliferation-and osteogenesis-related markers. Semi-quantitative PCR and Western blot analysis were used to analyze the expression levels of BMP9 in 143B cells after treated with ATRA alone or combined with BMP9. Results         In the 143B cells, ATRA significantly inhibited the proliferation of 143B cells (P<0.05) compared with the untreated group, promoted cell cycle arrested in G1 phase (P<0.05), and reduced the protein level of PCNA in a concentration-dependent manner. The protein levels of lately osteogenic makers OPN and OCN were up-regulated by ATRA treatment in a concentration-dependent manner (P<0.05), so were the mRNA and protein levels of endogenous BMP9 (P<0.05). BMP9 alone failed to up-regulate the expression of OPN and OCN but promoted the proliferation of 143B cells (P<0.05), but when combined with ATRA, BMP9 enhanced the expression of osteogenic markers and anti-proliferation effect induced by ATRA (P<0.05). Conclusion        ATRA inhibit the proliferation and induce osteogenic differentiation in 143B cells, and may restore the osteogenic capability of BMP9.

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更新日期/Last Update: 2017-07-11