[1]田谧,李远,王燕,等.重庆市永川区3~6岁留守儿童25羟维生素D水平及其与维生素D受体基因FOK Ⅰ位点多态性的相关性研究[J].第三军医大学学报,2017,39(05 ):493-498.
 Tian M,Li Yuan,Wang Yan,et al.Serum levels of 25-hydroxy vitamin D in 3 to 6-year-old left-behind children in Yongchuan District and its correlation with vitamin D receptor FOKⅠ genetic polymorphism[J].J Third Mil Med Univ,2017,39(05 ):493-498.
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重庆市永川区3~6岁留守儿童25羟维生素D水平及其与维生素D受体基因FOK Ⅰ位点多态性的相关性研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第05期
页码:
493-498
栏目:
公共卫生与预防医学
出版日期:
2017-03-15

文章信息/Info

Title:
Serum levels of 25-hydroxy vitamin D in 3 to 6-year-old left-behind children in Yongchuan District and its correlation with vitamin D receptor FOKⅠ genetic polymorphism
作者:
田谧李远王燕龚放
重庆医科大学附属永川医院:儿科,中心实验室
Author(s):
Tian M Li Yuan Wang Yan Gong Fang

Department of Pediatrics, Center for Clinical Laboratory, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160, China

关键词:
留守儿童25羟维生素D维生素D受体基因多态性
Keywords:
left-behind children 25-hydroxy vitamin D vitamin D receptor polymorphism
分类号:
R153.2; R195.2; R394.5
文献标志码:
A
摘要:

目的      了解重庆市永川区乡镇3~6岁留守儿童维生素D营养状况,探讨维生素D受体基因FOK Ⅰ位点多态性与25-羟维生素D[25-(OH)D]的相关性。方法      2015年11月-2016年4月期间,由重庆医科大学附属永川医院招募永川区部分乡镇3~6岁留守儿童进行健康体检,以非留守儿童作为对照,采用液相色谱串联质谱法进行血清25-(OH)D水平检测,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)法进行FOK Ⅰ位点基因型检测。结果      ①285例3~6岁留守儿童血清25-(OH)D平均水平为(19.566±5.185)ng/mL,维生素D充足占2.81%;不足组占41.40%;缺乏占52.98%;严重缺乏占2.81%。留守儿童组血清25-(OH)D较之非留守儿童组低,但两组比较无显著差异。其中3岁组25-(OH)D平均水平最高,各年龄组维生素D水平比较差异有统计学意义(P<0.001)。②ff基因型的维生素D水平较其余2种基因型低,但3种基因型的维生素D水平差异无显著统计学意义(P>0.05)。采用协方差分析校正年龄对维生素D水平的影响后,各基因型维生素D水平相比较差异仍无显著统计学意义(P>0.05)。结论      重庆市永川区留守儿童25-(OH)D水平严重低下,需采取措施提高维生素D水平,VDR受体基因FOK Ⅰ位点多态性可能与25-(OH)D水平无关。

Abstract:

Objective     To investigate the status and deficiency of 25-hydroxy vitamin D [25-(OH)D] in left-behind children at 3~6 years old living in Yongchuan District, Chongqing, and to explore the correlation of vitamin D receptor (VDR) genetic polymorphism FOK Ⅰ and serum levels of 25-(OH)D3. Methods      A total of 285 left-behind children at the age of 3 to 6 living in some towns of Yongchuan District were recruited in our hospital during November 2015 to April 2016. They all took physical examination, and their serum level of 25-(OH)D was detected by liquid chromatography-tandem mass spectrometry. The genotypes of FOK Ⅰ polymorphisms for VDR gene were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results      ① The average serum level of 25-(OH)D was 19.566±5.185 ng/mL in the 285 children, with 8 cases (2.81%) sufficient, 118 cases (41.40%) insufficient, 151 cases (52.98%) deficient and 8 cases (2.81%) severely deficient. The level was lower in the leftbehind children than the non-left-behind children, though no statistical difference. The level was the highest in the 3-yea-old group, and statistical differences were observed among the different age groups (P<0.001). ② The serum level of 25-(OH)D was lower in the children with ff genotype than those with Ff and FF genotypes, but there was no statistical difference among different genotypes groups (P>0.05). Covariance analysis indicated that there was no statistical difference in the serum level of 25-(OH)D among the different genotypes groups after adjustment of age (P>0.05). Conclusion      The serum level of 25-(OH)D is severely low in the left-behind children at the age of 3 to 6 in Yongchuan District. Some measures should be implied to improve the levels. Fok I polymorphisms in VDR gene might have no correlation with the serum level of 25-(OH)D.

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更新日期/Last Update: 2017-03-10