[1]汪超,蒋文斌,薛艳霞,等.SOCS1基因单核苷酸多态性与凉山彝族人群肺结核发生风险的关联性研究[J].第三军医大学学报,2017,39(03):291-295.
 Wang Chao,Jiang Wenbin,Xue Yanxia,et al.Association of single nucleotide polymorphisms of suppressor of cytokine signaling 1 gene with pulmonary tuberculosis in Liangshan Yi ethnic minority[J].J Third Mil Med Univ,2017,39(03):291-295.
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SOCS1基因单核苷酸多态性与凉山彝族人群肺结核发生风险的关联性研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第03期
页码:
291-295
栏目:
公共卫生与预防医学
出版日期:
2017-02-15

文章信息/Info

Title:
Association of single nucleotide polymorphisms of suppressor of cytokine signaling 1 gene with pulmonary tuberculosis in Liangshan Yi ethnic minority
作者:
汪超蒋文斌薛艳霞王娇罗杰徐欢杨成陈鸣邓少丽
第三军医大学大坪医院野战外科研究所检验科;中国人民解放军第519医院:检验科,医务处;西昌市人民医院检验科
Author(s):
Wang Chao Jiang Wenbin Xue Yanxia Wang Jiao Luo Jie Xu Huan Yang Cheng Chen Ming Deng Shaoli

Department of Clinical Laboratory, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042; Department of Clinical Laboratory, 3Department of Medical Affairs, No. 519 Hospital of PLA, Xichang, Sichuan Province, 615000; 3Department of Clinical Laboratory, Xichang People’s Hospital, Xichang, Sichuan Province, 615000, China

关键词:
肺结核细胞因子信号传导抑制因子1单核苷酸多态性遗传关联彝族
Keywords:
分类号:
R181.33; R394.5; R521
文献标志码:
A
摘要:

目的       研究SOCS1基因的2个标签单核苷酸多态性位点(tag single nucleotide polymorphisms,Tag SNP) rs243327、rs33932899与凉山彝族人群肺结核发生风险的遗传关联性。方法        2015年3月至2016年6月在凉山彝族自治州共采集188例彝族肺结核患者(肺结核组)和229例彝族健康人(健康对照组)全血样本。运用直接测序法对rs243327、rs33932899进行基因分型,应用哈-温平衡检验所选样本的群体代表性,采用卡方检验比较肺结核组和健康对照组之间基因型和等位基因频率分布的差异,评价SOCS1基因SNP与肺结核发生风险的关联性。结果        rs243327、rs33932899在肺结核组和健康对照组中的基因型分布均符合哈-温平衡。在rs243327位点处肺结核组CC基因型频率显著高于健康对照组(CC vs CT: OR=1.611, 95%CI=1.058-2.453, P=0.026; CC vs CT+TT: OR=1.651, 95%CI= 1.107-2.461, P=0.014),提示rs243327位点CC基因型可能增加凉山彝族人群肺结核的发生风险,可能是凉山彝族人群肺结核的易感基因型;C等位基因频率亦显著高于健康对照组(C vs T: OR=1.516, 95%CI=1.089-2.109, P=0.013),提示C等位基因可能是凉山彝族人群肺结核的易感等位基因。在rs33932899位点处基因型和等位基因分布在肺结核组和健康对照组之间均无统计学差异(GG vs CG:OR=1.148, 95%CI=0.762-1.731, P=0.436; GG vs CC: OR=2.184, 95%CI=0.874-5.454, P=0.088; GG/CG+CC: OR=1.229, 95%CI=0.875-1.928, P=0.193; G vs C: OR=1.290, 95%CI=0.932-1.785, P=0.124),提示rs33932899位点可能与凉山彝族人群肺结核的发生风险无相关性。 结论        SOCS1基因单核苷酸多态性可能与凉山彝族人群肺结核的发生发展存在遗传关联。

Abstract:

Objective       To investigate the genetic association of 2 tag single nucleotide polymorphisms (tagSNP) of suppressor of cytokine signaling 1 (SOCS1) gene, rs243327 and rs33932899 with the risk of pulmonary tuberculosis in the people of Yi ethnic minority living in Liangshan, Sichuan Province. Methods        A total of 188 patients of Yi nationality with pulmonary tuberculosis and 229 Yi healthy people were enrolled in this study. Their blood samples were collected during March 2015 and June 2016 in Liangshan Yi Autonomous Prefecture. Two tagSNP polymorphisms were analyzed by gene sequencing, and the genotype and allele frequency between pulmonary tuberculosis group and healthy control group were determined by Chi square test. Results       Both rs243327 and rs3393289 showed no deviation from Hardy-Weinberg equilibrium (HWE) in either the case or the control. The results showed that there was a significant correlation between rs243327 polymorphism and pulmonary tuberculosis, and the frequency of CC genotype was higher in pulmonary tuberculosis patients than healthy control (CC vs CT: OR=1.611, 95%CI: 1.058~2.453, P=0.026; CC vs CT+TT: OR=1.651, 95%CI: 1.107~2.461, P=0.014), indicating that CC carriers of rs243327 may increase the risk of pulmonary tuberculosis in Yi people, and had genetic susceptibility of tuberculosis. Meanwhile, the frequency of C allele was also higher in the patients than the healthy control (C vs T: OR=1.516, 95%CI: 1.089~2.109, P=0.013), and C allele might be susceptible allele for pulmonary tuberculosis. While there was no significant correlation between rs33932899 polymorphism and pulmonary tuberculosis (GG vs CG: OR=1.148, 95%CI: 0.762~1.731, P=0.436; GG vs CC: OR=2.184, 95%CI: 0.874~5.454, P=0.088; GG vs CG+CC: OR=1.229, 95%CI: 0.875~1.928, P=0.193; G vs C: OR=1.290, 95%CI: 0.932~1.785, P=0.124). This indicated that rs33932899 was not associated with the susceptibility of pulmonary tuberculosis.  Conclusion        rs243327 polymorphism may be associated with the occurrence and development of pulmonary tuberculosis in the Liangshan Yi population.

参考文献/References:

[1]Nair N, Cooreman E. Working towards TB elimination the WHO Regional Strategic Plan (2006-2015)[J].J Commum Dis, 2006, 38(3): 185-190.
[2]Comstock G W. Tuberculosis in twins: a re-analysis of the prophit survey[J].Am Rew Respir Dis, 1978, 117(4): 621-624. DOI:10.1164/arrd.1978.117.4.621
[3]Di Pietrantonio T, Schurr E. Hostpathogen specificity in tuberculosis[J]. Adv Exp Med Biol, 2013, 783: 33-44. DOI:10.1007/978-1-4614-6111-1_2
[4]周莹,高琪乐,黄华艺,等.宿主基因多态性与结核易感性研究进展[J].中华医院感染学杂志,2016, 26(2): 477-480. DOI : 10.11816/cn.ni.2016150859
[5]周江,张万江.甘露聚糖结合凝集素基因A/B位点与新疆维吾尔族结核病的相关性研究[J].中国防痨杂志, 2012, 34(7): 445-451.
[6]王喜,任玲君,李秀玲,等.新疆维吾尔族人群HLADR、DQ基因多态性与结核病易感性的研究[J].中国防痨杂志, 2011, 33(4): 197-203.
[7]吴芳.结核易感基因多态性与新疆哈萨克族结核病易感性的相关性研究[D].武汉:华中科技大学,2014.
[8]陈雪融, 冯玉麟,马玙,等.维生素D受体基因多态性与中国藏族结核病的关联研究[J].四川大学学报(医学版), 2006, 37(6): 847-851.
[9]李素芝,蒋亚建,谢本维,等.中国藏族人群HLA-DRB1、-DQA1、-DQB1等位基因多态性与肺结核易感性的关联性研究[J].第三军医大学学报,2011,33(12):1254-1257.DOI: 10.16016/j.1000-5404.2011.12.007
[10]Masood K I, Rottenberg M E ,Carow B. et al. SOCS1 gene expression is increased in severe pulmonary tuberculosis[J].Scand J Immunol, 2012, 76(4): 398-404. DOI:10.1111/j.13653083.2012.02731.x
[11]Nicol A F,Nuovo G J, CoelhoJ M, et al. SOCS in situ expression in tuberculous lymphadenitis in an endemic area[J]. Exp Mol Pathol, 2008, 84(3): 240-244. DOI:10.1016/j.yexmp.2008.04.001
[12]Masood K I, Hussain R, Rao N, et al.Differential Early Secreted Antigen Target (ESAT) 6 kDa--induced IFNgamma and SOCS1 expression distinguishes latent and active tuberculosis[J].J Infect Dev Ctries, 2014, 8(1): 59-66. DOI: 10.3855/jidc.3412
[13]Yang S, Li F, Jia S, et al. Early secreted antigen ESAT-6 of Mycobacterium Tuberculosis promotes apoptosis of macrophages via targeting the microRNA155SOCS1 interaction[J]. Cell Physiol Biochem,2015, 35(4): 1276-1288. DOI:10.1159/000373950
[14]Vargas-Alarcon G, Posadas-Sanchez R, Posadas-Romero C,et al.Association of the suppressor of cytokine signaling 1 (SOCS1) gene polymorphisms with acute coronary syndrome in Mexican patients[J]. Mol Immunol, 2014,62(1):137-141. DOI:10.1016/j.molimm.2014.06.019
[15]Zhang P, Li F,Li N,et al. Genetic variations of SOCS1 are associated with chronic hepatitis B virus infection[J]. Human Immunology, 2014,75(8):709-714. DOI:10.1016/j.humimm.2014.04.010

更新日期/Last Update: 2017-02-07