[1]司海超,司小萌,刘展.槲皮素减轻坐骨神经慢性缩窄性损伤大鼠的神经病理性疼痛及其相关机制[J].第三军医大学学报,2017,39(01):54-59.
 Si Haichao,Si Xiaomeng,Liu Zhan.Effects and mechanism of quercetin on neuropathic pain in chronic constriction injury rats[J].J Third Mil Med Univ,2017,39(01):54-59.
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槲皮素减轻坐骨神经慢性缩窄性损伤大鼠的神经病理性疼痛及其相关机制(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第01期
页码:
54-59
栏目:
基础医学
出版日期:
2017-01-15

文章信息/Info

Title:
Effects and mechanism of quercetin on neuropathic pain in chronic constriction injury rats
作者:
司海超司小萌刘展
河南省南阳市中心医院麻醉科
Author(s):
Si Haichao Si Xiaomeng Liu Zhan

Department of Anesthesiology, Nanyang Centeral Hospital, Nanyang, Henan Province, 473009, China

关键词:
槲皮素坐骨神经慢性缩窄性损伤神经病理性疼痛
Keywords:
quercetin chronic constriction injury neuropathic pain
分类号:
R441.1; R651.3; R971.1
文献标志码:
A
摘要:

目的     探究槲皮素对坐骨神经慢性缩窄性损伤(chronic constriction injury,CCI)模型大鼠神经病理性疼痛的影响及其机制。方法     构建CCI大鼠模型,用不同浓度槲皮素干预,通过行为学实验检测机械缩足反射阈值(MWT)和热缩足反射潜伏期(TWL),ELISA试剂盒检测大鼠脊髓中炎性因子的表达,Western blot和qRTPCR分别检测iNOS、COX2和Wnt3a、βcatenin的蛋白以及mRNA的表达。结果     与对照组相比,模型组大鼠MWT和TWL值明显下降(P<0.05),脊髓TNF-α、IL-1β,疼痛相关分子iNOS、COX-2,WNT通路Wnt3a、β-catenin蛋白水平均显著上升(P<0.05),而槲皮素使模型组大鼠MWT和TWL值显著提高,TNF-α和IL-1β,iNOS和COX-2,Wnt3a和β-catenin水平均显著下降(P<0.05),并呈现一定的剂量依赖效应。而Wnt/β-catenin通路激活剂可阻断槲皮素对CCI大鼠的作用。结论     槲皮素可能通过抑制Wnt/βcatenin通路及其下游靶分子COX-2和iNOS的表达减轻CCI大鼠的神经病理性疼痛。

Abstract:

Objective      To determine the effects of quercetin (Que) on neuropathic pain in rats after chronic constriction injury (CCI), and investigate its underlying mechanisms. Methods      A total of 80 SD rats were randomly divided into control (n=8), sham operation (n=18), and CCI model group (n=56). In 7 d after model establishment, Que was given intragastrically at 30, 50 and 100 mg/kg. Before and in 3, 7, 14, 21 and 28 d after treatment , the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were determined by behavioral test in neuropathic pain rat models of CCI. Spinal production of TNF-α and IL-1β was quantified by ELISA kits. The protein and mRNA expression levels of iNOS, COX-2, Wnt3a and β-catenin were determined by qRT-PCR and Western blotting, respectively. Results      Compared with control and sham operation group, the MWT and TWL were decreased significantly in the CCI group (P<0.05), while the spinal contents of TNF-α and IL-1β, the expression levels of the pain associated molecules iNOS and COX-2, and WNT pathway molecules, Wnt3a and βcatenin were all up-regulated (P<0.05). However, Que treatment also markedly increased the MWT and TWL, inhibited the production of TNF-α and IL-1β, and suppressed the expression of iNOS, COX-2, Wnt3a and β-catenin (P<0.05), in a dose-dependent manner. Whereas, the activator of Wnt/β-catenin pathway could block the effect of Que to the CCI rats. Conclusion      Que may alleviate neuropathic pain in CCI rats by inhibiting Wnt/β-catenin signal pathway and downstream molecules COX-2 and iNOS.

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更新日期/Last Update: 2017-01-03