[1]邓红,高静,廖林,等.七氟烷预处理对大鼠肺缺血再灌注损伤肺组织细胞自噬水平的影响[J].第三军医大学学报,2016,38(20):2264-2268.
 Deng Hong,Gao Jing,Liao Lin,et al.Effects of sevoflurane preconditioning on autophagy in lung ischemiareperfusion injury in rats[J].J Third Mil Med Univ,2016,38(20):2264-2268.
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七氟烷预处理对大鼠肺缺血再灌注损伤肺组织细胞自噬水平的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第20期
页码:
2264-2268
栏目:
基础医学
出版日期:
2016-10-30

文章信息/Info

Title:
Effects of sevoflurane preconditioning on autophagy in lung ischemiareperfusion injury in rats
作者:
邓红高静廖林刘金碧鲁开智郑洪易斌
遵义市第一人民医院麻醉科;第三军医大学西南医院麻醉科;綦江区人民医院麻醉科
Author(s):
Deng HongGao JingLiao LinLiu JinbiLu KaizhiZheng HongYi Bin

Department of Anesthesiology, the First People’s Hospital of Zunyi,Zunyi, Guizhou Province,563000;Department of Anesthesiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038;Department of Anesthesiology, Qijiang Distract People’s Hospital, Chongqing,401420, China

关键词:
七氟烷肺缺血再灌注损伤自噬PI3K信号通路
Keywords:
sevofluranelung ischemiareperfusion injuryautophagyPI3K
分类号:
R322.35;R364.12;R971.2
文献标志码:
A
摘要:

目的     探讨七氟烷预处理对大鼠肺缺血再灌注损伤(ischemiareperfusion injury,IRI)肺组织中细胞自噬的影响。方法      健康成年雄性SD 大鼠75只,采用开胸夹闭左肺门建立IRI模型,将75只大鼠编号后按随机数字表法分为5组:假手术组(Sham),缺血再灌注组(I/R),七氟烷预处理组(SEVO),LY294002组(LY)及七氟烷预处理+LY294002组(PI3K抑制剂)(SEVO+ LY),各15只。缺血1 h,再灌注120 min后处死各组大鼠,取出肺组织,检测大鼠肺组织湿/干重比(W/D),Western blot法测定肺组织Beclin1、LC3Ⅱ/Ⅰ、P62及pPI3K表达。结果      与Sham组比较,I/R组肺组织发生严重水肿(P<0.05),且肺组织细胞自噬水平增加,自噬相关蛋白Beclin-1、LC3Ⅱ/Ⅰ、P62蛋白表达上调(P<0.05);与I/R组比较,SEVO组肺水肿明显减轻(P<005),Beclin-1、LC3Ⅱ/Ⅰ、P62蛋白表达下调,而pPI3K表达增加(P<0.05),LY组与I/R组比较差异没有统计学意义(P>005);与SEVO组比较,SEVO+LY组中肺水肿明显加重,Beclin-1、LC3Ⅱ/Ⅰ、P62蛋白表达上调而pPI3K表达下调(P<0.05)。结论      七氟烷预处理通过激活PI3K信号转导通路,恢复LIRI期间细胞自噬流,减少自噬性细胞死亡从而对肺缺血再灌注损伤起保护作用。

Abstract:

Objective      To determine the effects of sevoflurane preconditioning on function of autophagy in lung ischemiareperfusion(I/R) injury in rats.   Methods       A lung I/R injury model in rats was established by clamping of the left hilum after thoracotomy.A total of 75 healthy adult male Sprague-Dawley (SD) rats (SPF grade) were randomly divided into 5 groups(n=15): sham operation group(Sham), ischemia-reperfusion group(I/R), sevoflurane preconditioning group(SEVO), LY294002(LY, PI3K inhibitor) group, sevoflurane preconditioning+LY294002 group(SEVO+LY). After 1 h of ischemia and 120 min of reperfusion,the rats were executed and the left lung tissue were harvested for wet-dry weight (W/D)measurement. Western blotting was used to detect the expression of Beclin-1, LC3Ⅱ/Ⅰ, P62 and p-PI3K.  Results       Compared with the Sham group, I/R group developed severe lung edema (P<0.05), and had increased level of autophagy and its related protein expression of Beclin1,LC3Ⅱ/Ⅰ,P62 (P<0.05). The pulmonary injury and increased protein expression found in I/R group were significantly reduced in SEVO group (P<0.05). Only p-PI3K expression was significantly increased in the SEVO group than the I/R group (P<0.05).There were no significant differences between LY group and I/R group in these respects(P>0.05). In SEVO+LY group, pulmonary injury was aggravated.The expression of Beclin-1, LC3Ⅱ/Ⅰ, P62 was up-regulated and p-PI3K level was remarkably decreased compared with SEVO group(P<0.05). Conclusion       The results demonstrated that autophagy was involved in the lung I/R pathophysiological process. Sevoflurane preconditioning can protect the lung I/R injury in rats, which is likely related to activation of PI3K signaling transduction and restoration of autophagic flux,at last reducing autophagic cell death.

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更新日期/Last Update: 2016-10-24