[1]魏佳鑫,范霞,张莹,等.6甲酰基吲哚并[3,2-b]咔唑对脂多糖诱导的急性呼吸窘迫综合征大鼠的保护作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(22):2419-2423.
 Wei Jiaxin,Fan Xia,Zhang Ying,et al.Protective effects of 6-formylindolo[3,2-b]carbazole against LPS-induced acute respiratory distress syndrome in rats[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(22):2419-2423.
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6甲酰基吲哚并[3,2-b]咔唑对脂多糖诱导的急性呼吸窘迫综合征大鼠的保护作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第22期
页码:
2419-2423
栏目:
基础医学
出版日期:
2016-11-30

文章信息/Info

Title:
Protective effects of 6-formylindolo[3,2-b]carbazole against LPS-induced acute respiratory distress syndrome in rats
作者:
魏佳鑫范霞张莹陈浩黄文娟梁华平
遵义医学院附属医院重症医学科;第三军医大学大坪医院野战外科研究所第一研究室,创伤、烧伤与复合伤国家重点实验室
Author(s):
Wei JiaxinFan XiaZhang Ying Chen HaoHuang WenjuanLiang Huaping

Department of Critical Care Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou Povince,  563003; State Key Laboratory of Trauma, Burns and Combined Injury, Department 1, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China

关键词:
6甲酰基吲哚并[32-b]咔唑脂多糖急性呼吸窘迫综合征芳香烃受体NF-&kappaB
Keywords:
6-formylindolo(32-b)carbazole lipopolysaccharide acute respiratory distress syndrome aryl hydrocarbon receptor NF-&kappaB
分类号:
R-332;R563.8;R977.6
文献标志码:
A
摘要:

目的      探讨芳香烃受体(aryl hydrocarbon receptor,AhR)内源性配体6-甲酰基吲哚并 [3,2-b]咔唑( 6-formylindolo[3,2-b]carbazole,FICZ)对脂多糖(lipopolysaccharide,LPS)诱导的大鼠急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)的保护作用及其机制。方法     按照随机数字表法将24只SD大鼠分为正常对照组、FICZ对照组、LPS模型组和FICZ治疗组,每组6只。通过气管滴注LPS(3 mg/kg)制备ARDS模型,并于建模后6 h收集各组肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及肺组织,通过肺湿/干质量比值、HE 染色考察各组肺组织的损伤情况;采用酶联免疫吸附试验(ELISA)检测BALF和肺组织中炎症因子(IL-1β、TNF-α)水平及肺组织中髓过氧化物酶(myeloperoxidase,MPO)含量;利用Western blot检测肺组织中AhR和NF-κB p65的蛋白表达。结果     与LPS模型组相比,FICZ可有效减轻LPS所致的肺水肿及组织病理学损伤,减少BALF和肺组织中炎症因子TNF-α、IL-1β的释放及肺组织中MPO的含量。另外,FICZ在激活AhR的同时对LPS作用下肺组织胞核中NF-κB p65的升高起到明显的抑制作用。结论           FICZ对LPS诱导的大鼠ARDS具有保护作用,其作用可能是通过激活AhR以减少NF-κB p65的入核来实现的。

Abstract:

Objective     To determine the protective effect of 6-formylindolo(3,2-b)carbazole (FICZ), an endogenous ligand of aryl hydrocarbon receptor (AhR), against lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) in rats. Methods     Twenty-four adult SD rats were randomly divided into 4 groups (n=6 per group), that is, control group, FICZ control group, LPS model group and FICZ treatment group. ARDS model was set up via intratracheal injection of LPS (3 mg/kg). The bronchoalveolar lavage fluid (BALF) and lung tissue were collected at 6 h after modeling. The injury of lung was characterized by wet/dry ratio and H&E staining. The contents of inflammatory cytokines interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) as well as myeloperoxidase (MPO) in the lung tissue and/or BALF were determined by enzyme-linked immunosorbent assay (ELISA). AhR and NF-κB p65 expression in the lung tissue was detected by Western blotting. Results     Compared with LPS model group, FICZ treatment significantly ameliorated LPS-induced pulmonary edema and lung tissue injury, down-regulated the levels of TNF-α and IL-1β in both BALF and lung tissue, and decreased MPO content in lung tissue. In addition, FICZ could activate AhR and remarkably suppress the LPS-induced increment of nucleic NF-κB p65 in the necleus of lung tissue. Conclusion      FICZ exerts protective effects against LPS-induced lung injury in rat ARDS model via activating AhR and suppressing NF-κB p65 nuclear translocation.

更新日期/Last Update: 2016-11-23