[1]张炜炜,刘瑾,陈伟.犬肾脏脱细胞基质凝胶的制备及生物相容性实验研究[J].第三军医大学学报,2015,37(19 ):1942-1945.
 Zhang Weiwei,Liu Jin,Chen Wei.Preparation and biocompatibility of canine kidney acellular matrix gel[J].J Third Mil Med Univ,2015,37(19 ):1942-1945.
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犬肾脏脱细胞基质凝胶的制备及生物相容性实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第19期
页码:
1942-1945
栏目:
专题报道
出版日期:
2015-10-15

文章信息/Info

Title:
Preparation and biocompatibility of canine kidney acellular matrix gel
作者:
张炜炜刘瑾陈伟
第三军医大学大坪医院野战外科研究所肾脏内科;重庆市人民医院病理科;第三军医大学新桥医院泌尿外一科
Author(s):
Zhang Weiwei Liu Jin Chen Wei

Department of Nephrology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042; Department of Pathology, Chongqing People’s Hospital, Chongqing, 400013; Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

关键词:
肾脏脱细胞基质凝胶组织工程
Keywords:
kidneys acellular matrix gel tissue engineering
分类号:
R322.61; R392-33; R329.25
文献标志码:
A
摘要:

目的      制备犬肾脏脱细胞基质凝胶,检测肾脏脱细胞基质凝胶与肾小管上皮细胞的生物相容性。      方法       取10~12 kg成年雄性比格犬肾脏,进行脱细胞处理,制备肾脏脱细胞基质凝胶,利用HE染色DAPI染色观察其是否有细胞残留,扫描电镜观察结构,进行DNA、糖胺聚糖(GAGs)含量检测,对肾小管上皮细胞株(HK-2细胞)进行培养,检测肾脏脱细胞基质凝胶的生物相容性。      结果       脱细胞处理后的肾脏组织仅留下细胞外基质,无明显细胞核残留;制备的脱细胞基质凝胶呈白色半透明胶状,扫描电镜下呈网状、多孔结构,富含GAGs,并有效去除DNA,体外促进HK-2细胞的生长。      结论      成功制备了犬肾脏脱细胞基质凝胶,该凝胶能够促进肾小管上皮细胞的生长。

Abstract:

Objective       To prepare a gel form from acellular renal extracellular matrix (ECM), and investigate its biocompatibility with renal tubular epithelial cells.       Methods       The kidneys were harvested from 10~12 kg adult male Beagle dogs. After decellularization, a gel was produced from the ECM. HE staining and DAPI staining were used to observe whether there were residual cells. Scanning electron microscopy (SEM) was employed to study the microstructure of the obtained gel. The contents of DNA and glycosaminoglycan (GAGs) were measured. Human renal tubular epithelial HK-2 cells were cultured with the gel to detect the biocompatibility of ECM gel.       Results      After decellularization of the kidneys, only the extracellular matrix was preserved. No visible cell nuclei were seen in the matrix. SEM showed that the ECM gel displayed a reticular and porous morphology. GAGs were well preserved and DNA was effectively removed in the gel. The ECM gel promoted the proliferation of HK-2 cells in vitro.       Conclusion       A gel form from canine acellular renal ECM is successfully prepared in this study, and it might promote the proliferation of renal tubular epithelial cells.

参考文献/References:

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更新日期/Last Update: 2015-09-28