[1]林斌,董兴有,赵江,等.二甲基亚砜通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎[J].第三军医大学学报,2015,37(06):505-509.
 Lin Bin,Dong Xingyou,Zhao Jiang,et al.Dimethyl sulfoxide alleviates protamine sulfate-induced rat cystitis by reducing inflammation and oxidative stress[J].J Third Mil Med Univ,2015,37(06):505-509.
点击复制

二甲基亚砜通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第06期
页码:
505-509
栏目:
专题报道
出版日期:
2015-03-30

文章信息/Info

Title:
Dimethyl sulfoxide alleviates protamine sulfate-induced rat cystitis by reducing inflammation and oxidative stress
作者:
林斌董兴有赵江王亮龙州朱景振丁国林李龙坤
第三军医大学新桥医院泌尿外科
Author(s):
Lin Bin Dong Xingyou Zhao Jiang Wang Liang Long Zhou Zhu Jingzhen Ding Guolin Li Longkun

Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

关键词:
二甲基亚砜间质性膀胱炎鱼精蛋白膀胱炎
Keywords:
dimethyl sulfoxide interstitial cystitis protamine sulfate cystitis
分类号:
R694.305;R965;R983
文献标志码:
A
摘要:

目的 探讨二甲基亚砜(dimethyl sulfoxide, DMSO)对鱼精蛋白(protamine sulfate,PS)诱导的大鼠间质性膀胱炎(interstitial cystitis,IC)治疗作用。 方法 SD雌性大鼠48只,按随机数字表法分为正常组、PS灌注组、PS+DMSO组、盐水灌注组(NS组),每组12只大鼠。正常组大鼠未给予任何处理;PS组用0.5 mL, 30 mg/mL的PS膀胱内灌注并在膀胱内保留30 min;PS+DMSO组使用50% DMSO溶解PS,按照0.5 mL, 30 mg/mL的 PS膀胱内灌注并在膀胱内保留30 min;NS组用0.5 mL生理盐水膀胱灌注并保留30 min,各组大鼠每周处理1次,连续处理4周。4周后,HE和甲苯胺蓝染色用于病理学评分和肥大细胞计数;尿动力学和肌条实验被用于评价大鼠膀胱功能和肌肉收缩性改变;Western blot检测SOD2、 GSH-Px 、 IL-6、IL-1B、TNF-α的表达量。 结果 与PS组相比,PS+DMSO组大鼠膀胱病理学评分[(3.00±0.00) vs(0.83±0.40),P<0.05]、肥大细胞计数[(19.66±2.16) vs (6.16±1.16), P<0.05]以及炎症因子IL-6、IL-1B和TNF- α表达(P<0.05)显著降低,而抗氧化应激酶(SOD2和GSH)显著恢复(P<0.05);在膀胱功能学上,与PS组相比,PS+DMSO组大鼠膀胱排尿频率[(24.83±3.06)vs (9.83±1.47),P<0.05]显著降低,排尿间隔[(2.42±0.37)vs (6. 40±0.57),P<0.05]显著延长,肌肉收缩频率[(6.93±0.97)vs (4.94±0.73),P<0.05]显著降低。 结论 DMSO 可能通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎。

Abstract:

Objective      To investigate the effect of dimethyl sulfoxide (DMSO) on the treatment of protamine sulfate (PS)-induced interstitial cystitis in rats.       Methods      Totally, 48 SD rats were randomized into a normal group, a PS perfusion group, a PS+DMSO group, and a saline perfusion group (NS group), 12 rats in each group. The normal group was not given any treatment. The PS group was treated with 0.5 mL, 30 mg/mL PS by bladder perfusion for 30 min. The PS+DMSO group was treated with 0.5 mL, 30 mg/mL PS+DMSO (PS dissolved in 50% DMSO) by bladder perfusion for 30 min. The NS group was treated with 0.5 mL normal saline by bladder perfusion for 30 min. The rats were treated once in a week and the treatment lasted for 4 weeks. After 4 weeks, HE staining and toluidine blue staining were used for pathological scoring and mast cell counting. Urodynamics and muscle strip test were used for the evaluation of bladder function and muscle contraction. The changes of superoxide peroxidase 2 (SOD2), glutathione peroxidase (GSH-Px), interleukin 6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were measured by Western blotting.       Results      Compared with the PS group, the rat bladder pathological scores (3.00±0.00 vs 0.83±0.40, P<0.05), mast cell count (19.66±2.16 vs 6.16±1.16, P<0.05), and expression of inflammatory cytokines (IL-6, IL-1β and TNF-α, P<0.05) were significantly decreased in the PS+DMSO group, and the antioxidant enzymes (SOD2 and GSH-Px) were significantly increased (P<0.05). For bladder function, compared with the PS group, the PS+DMSO group showed lower micturition frequency (24.83±3.06 vs 9.83±1.47, P<0.05), shorter intercontraction interval (2.42±0.37 vs 6.40± 0.57, P<0.05), and lower muscle contraction frequency (6.93±0.97 vs 4.94±0.73, P<0.05).       Conclusion      DMSO alleviates the PS-induced rat cystitis by reducing inflammation and oxidative stress.

参考文献/References:

[1]Humphrey L, Arbuckle R, Moldwin R, et al. The bladder pain/interstitial cystitis symptom score: development, validation, and identification of a cut score[J]. Eur Urol, 2012, 61(2): 271-279.
[2]Nickel J C, Tripp D A, Pontari M, et al. Psychosocial phenotyping in women with interstitial cystitis/painful bladder syndrome: a case control study[J]. J Urol, 2010, 183(1): 167-172.
[3]Grover S, Srivastava A, Lee R, et al. Role of inflammation in bladder function and interstitial cystitis[J]. Ther Adv Urol, 2011, 3(1): 19-33.
[4]Cosar R, Eskiocak S, Yurut-Caloglu V, et al. Can radiation-induced chronic oxidative stress in kidney and liver be prevented by dimethylsulfoxide? Biochemical determination by serum and tissue markers[J]. J Buon, 2012, 17(1):160-167.
[5]Santos N C, Figueira-Coelho J, Martins-Silva J, et al. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects[J]. Biochem Pharmacol, 2003, 65(7): 1035-1041.
[6]Lv Y S, Yao Y S, Lin M E, et al. Interleukin-6 levels in female rats with protamine sulfate-induced chronic cystitis treated with hyaluronic acid [J]. Int J Urol, 2013, 20(10): 1017-1022.
[7]Chen W, Jiang C, Jin X, et al. Roles of stem cell factor on loss of interstitial cells of Cajal in bladder of diabetic rats[J]. Urology, 2011, 78(6): 1443. e1-1443. e6.
[8]李龙坤, 宋波, 金锡御, 等. 骶神经根电刺激对大鼠神经源性膀胱储尿功能影响的实验研究[J]. 第三军医大学学报, 2003, 25(22): 1972-1974.
[9]Li L, Jiang C, Hao P, et al. Changes of gap junctional cell-cell communication in overactive detrusor in rats[J]. Am J Physiol Cell Physiol, 2007, 293(5): C1627-C1635.
[10]Theoharides T C, Kempuraj D, Sant G R. Mast cell involvement in interstitial cystitis: a review of human and experimental evidence[J]. Urology, 2001, 57(6 Suppl 1): 47-55.
[11]Parsons C L. The role of the urinary epithelium in the pathogenesis of interstitial cystitis/prostatitis/urethritis[J]. Urology, 2007, 69(4 Suppl): 9-16.
[12]Nasrin S, Masuda E, Kugaya H, et al. Improvement by phytotherapeutic agent of detrusor overactivity, down-regulation of pharmacological receptors and urinary cytokines in rats with cyclophosphamide induced cystitis[J]. J Urol, 2013, 189(3): 1123-1129.
[13]Levine B, Mizushima N, Virgin H W. Autophagy in immunity and inflammation[J]. Nature, 2011, 469(7330): 323-335.
[14]Smith K J, Chess-Williams R, McDermott C. Luminal DMSO: effects on detrusor and urothelial/lamina propria function[J]. Biomed Res Int, 2014, 2014: 347616.
[15]王亮, 赵江, 董兴有, 等. 乙酰半胱氨酸对硫酸鱼精蛋白诱导的大鼠膀胱炎的作用[J]. 第三军医大学学报, 2014, 36(7): 640-644.
[16]Tyagi P, Hsieh V C, Yoshimura N, et al. Instillation of liposomes vs dimethyl sulphoxide or pentosan polysulphate for reducing bladder hyperactivity[J]. BJU Int, 2009, 104(11): 1689-1692.
[17]Soler R, Bruschini H, Truzzi J C, et al. Urinary glycosaminoglycans excretion and the effect of dimethyl sulfoxide in an experimental model of non-bacterial cystitis[J]. Int Braz J Urol, 2008, 34(4): 503-511.
 

相似文献/References:

[1]王永权,宋波,方强,等.女性间质性膀胱炎和膀胱过度活动症的尿动力学比较研究[J].第三军医大学学报,2009,31(11):1084.
 WANG Yong-quan,SONG Bo,FANG Qiang,et al.Urodynamic studies of interstitial cystitis and overactive bladder in women[J].J Third Mil Med Univ,2009,31(06):1084.
[2]李佳,夏六兵,张腾,等.4-PBA抑制内质网应激降低间质性膀胱炎大鼠膀胱兴奋性[J].第三军医大学学报,2016,38(11):1270.
 Li Jia,Xia Liubing,Zhang Teng,et al.4-PBA inhibits endoplasmic reticulum stress to down-regulate bladder excitation in protamine/lipopolysaccharide-induced interstitial cystitis in rats[J].J Third Mil Med Univ,2016,38(06):1270.
[3]李兆飞,徐晨,邢菲,等.阿米替林对大鼠间质性膀胱炎的治疗作用[J].第三军医大学学报,2013,35(18):1936.
 Li Zhaofei,Xu Chen,Xing Fei,et al.Therapeutic effect of amitriptyline on phenotype-induced interstitial cystitis in rats[J].J Third Mil Med Univ,2013,35(06):1936.
[4]王亮,赵江,董兴有,等.乙酰半胱氨酸对硫酸鱼精蛋白诱导的大鼠膀胱炎的作用[J].第三军医大学学报,2014,36(07):640.
 Wang Liang,Zhao Jiang,Dong Xingyou,et al.N-acetylcysteine attenuates protamine sulfate-induced cystitis in rats[J].J Third Mil Med Univ,2014,36(06):640.
[5]季惠翔,张家华,金锡御,等.间质性膀胱炎5例报告[J].第三军医大学学报,2002,24(07):0.[doi:10.16016/j.1000-5404.2002.07.012 ]

更新日期/Last Update: 2015-03-19