[1]曹小洁,张莉莉,郭露,等.oxLDL通过PPARγ-ABCG1通路促进血管平滑肌细胞泡沫化[J].第三军医大学学报,2015,37(04):325-329.
 Cao Xiaojie,Zhang Lili,Guo Lu,et al.OxLDL promotes vascular smooth muscle cells to form foam cells through PPARγ-ABCG1 signal pathway[J].J Third Mil Med Univ,2015,37(04):325-329.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第04期
页码:
325-329
栏目:
论著
出版日期:
2015-02-28

文章信息/Info

Title:
OxLDL promotes vascular smooth muscle cells to form foam cells through PPARγ-ABCG1 signal pathway
作者:
曹小洁张莉莉郭露黎炳护皮燕廖少琼龙春燕刘赟尹延伟李敬诚
第三军医大学大坪医院野战外科研究所神经内科
Author(s):
Cao Xiaojie Zhang Lili Guo Lu Li Binghu Pi Yan Liao Shaoqiong Long Chunyan Liu Yun Yin Yanwei Li Jingcheng

Department of Neurology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China

关键词:
泡沫细胞平滑肌细胞oxLDLPPAR&gammaABCG1
Keywords:
foam cells vascular smooth muscle cells oxLDL PPAR&gamma ABCG1
分类号:
R322.12;R329.26;R543.502
文献标志码:
A
摘要:

目的      探讨氧化低密度脂蛋白(oxLDL)促进血管平滑肌细胞(vascular smooth muscle cells,VSMCs)泡沫化是否与过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor γ, PPARγ)-三磷酸腺苷结合盒转运子G1(ATP-binding cassette transporter G1, ABCG1)通路调节相关。      方法      组织贴块法培养原代VSMCs;细胞免疫荧光染色鉴定原代VSMCs;BODIPY染色定性检测细胞内脂质沉积,酶促比色法定量检测细胞内总胆固醇沉积的量;Western blot检测VSMCs中PPARγ、ABCG1蛋白表达情况。      结果      ①OxLDL作用VSMCs 48 h后脂质沉积增加;②OxLDL作用VSMCs PPARγ蛋白表达量下降约53%(P<0.01),ABCG1蛋白表达量下降约48%(P<0.05);③罗格列酮激动PPARγ后,ABCG1蛋白表达量较单独的oxLDL组增加约147%(P<0.05),VSMCs中脂质沉积明显减少。      结论      oxLDL通过PPARγ-ABCG1通路促进血管平滑肌细胞泡沫化。

Abstract:

Objective      To investigate whether oxidized low-density lipoprotein (oxLDL) promoting vascular smooth muscle cells (VSMCs) to form foam cells is regulated by peroxisome proliferator receptor γ (PPARγ)-ATP-binding cassette transporter G1 (ABCG1) pathway.       Methods      Primary VSMCs were isolated from male C57BL/6J mice, cultured with tissue explant technique, and identified by immunofluorescence staining. BODIPY staining was used to analyze the intracellular lipid deposition, and the total cholesterol deposition in the VSMCs was quantitatively detected by enzymatic colorimetric assay. the protein expression of PPARγ and ABCG1 in the VSMCs was analyzed by Western blotting.       Results      After oxLDL intervention in the VSMCs for 48 h, the lipid deposition was increased. PPARγ protein expression was decreased by about 53% (P<0.01), while ABCG1 protein expression was decreased by about 48% (P<0.05). After rosiglitazone activated PPARγ, ABCG1 protein expression was increased by about 147% (P<0.05), and the lipid deposition was reduced in the VSMCs.       Conclusion      OxLDL promotes the formation of smooth muscle-derived foam cells through PPARγ-ABCG1 signal pathway.

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更新日期/Last Update: 2015-01-30