[1]胡欣春,魏益平,彭金华,等.miR-4686对A549细胞球增殖的调控作用及其机制研究[J].陆军军医大学学报(原第三军医大学学报),2014,36(04):340-345.
　Hu Xinchun,Wei Yiping,Peng Jinhua,et al.Regulation of miR-4686 on proliferation in A549 spheres[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(04):340-345.
点击复制

miR-4686对A549细胞球增殖的调控作用及其机制研究(/HTML )
分享到：

陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第04期
页码:
340-345
栏目:
论著
出版日期:
2014-02-28

文章信息/Info

Title:
Regulation of miR-4686 on proliferation in A549 spheres
作者:
胡欣春; 魏益平; 彭金华; 喻东亮; 徐建军
南昌大学第二附属医院心胸外科；南昌大学医学院；江西省胸科医院胸外科
Author(s):
Hu Xinchun;  Wei Yiping;  Peng Jinhua;  Yu Dongliang;  Xu Jianjun
Department of Cardiothoracic Surgery, Second Affiliated Hospital, Nanchang University, Nanchang, Jiangxi Province, 330006; Medicine School of Nanchang University, Nanchang, Jiangxi Province, 330006; Department of Chest Surgery, Jiangxi Chest Hospital, Nanchang, Jiangxi Province, 330006, China
关键词:
microRNA;  抑制物; CD133; 肺癌
Keywords:
microRNA;  inhibitors;  CD133;  lung cancer
分类号:
R73-362；R730.23；R734.2
文献标志码:
A
摘要:
目的      研究miR-4686对A549细胞球增殖的调控作用及其相关机制。      方法      培养A549细胞，从中分选出CD133+CD44+A549细胞并置于无血清培养基中培养，形成细胞球后进行CD133+CD44+表达检测。Western blot检测A549细胞和A549细胞球中Wnt5a、Wnt2蛋白的表达；合成miR-4686抑制物和阴性对照，分别转染A549细胞球48 h后，应用Real-time PCR检测转染前后miR-4686的表达变化后，使用Real-time PCR和Western blot分析Wnt5a、Wnt2以及β-catenin mRNA和蛋白的表达变化，利用MTT检测12、24、48 h细胞的增殖能力。      结果      流式细胞术检测A549细胞球CD133+CD44+表达为69.800%；A549细胞球分别转染miR-4686抑制物和阴性对照物后，miR-4686的表达以及Wnt5a、Wnt2和β-catenin mRNA和蛋白的表达较对照组和miR-4686阴性对照物组显著下调(P<0.05）；A549细胞球转染miR-4686抑制物后12、24、48 h，其OD值显著低于对照组和miR-4686对照物干预组（P<0.05）。      结论      miR-4686介导Wnt信号通路发挥调控A549细胞球增殖的作用，其可成为治疗肺癌的潜在靶点。
Abstract:
Objective      To study the mechanism of miR-4686 regulating the proliferation of A549 spheres.       Methods      CD133+CD44+A549 cells were sorted by FACS, and then cultured in serum-free medium. Positive rate of CD133+CD44+ A549 spheres were detected by FACS. Wnt5a and Wnt2 protein expression of A549 cells and A549 spheres were assayed by Western blotting. MiR-4686 inhibitors and negative control were synthesized. A549 spheres were treated with miR-4686 inhibitors and negative control separately for 48 h, and then the expression of miR-4686 were detected by real-time PCR. Wnt5a, Wnt2 and β-catenin mRNA and protein expression was assayed by RT-PCR and Western blotting, respectively. The proliferation of A549 spheres was detected by MTT assay at 12, 24 and 48 h.       Results      CD133+CD44+ A549 cells were sorted. The positive rate of CD133+CD44+ was 69.800% in A549 spheres. Wnt5a and Wnt2 protein expression was significant decreased in A549 cells, as compared to A549 spheres (P<0.05). After treatment with miR-4686 inhibitors, the expression of miR-4686 as well as the mRNA and protein expression of Wnt5a, Wnt2 and β-catenin were significant down-regulated, compared to the control group and miR-4686 negative control group (P<0.05). What’s more, OD values at 12, 24 and 48 h were decreased after miR-4686 inhibitor treatment (P<0.05).       Conclusion      MiR-4686 plays an important role in regulation of A549 sphere proliferation through Wnt signal pathway, which may become a novel target for treatment of non-small-cell lung cancer.

相似文献/References:

[1]张政,罗勇军,彭惠民,等.早期糖尿病肾病相关微小RNA在小鼠肾脏的表达变化[J].陆军军医大学学报(原第三军医大学学报),2010,32(13):1383.
　Zhang Zheng,Luo Yongjun,Peng Huimin,et al.Expression of early diabetic nephropathy-related microRNA in kidneys of diabetic mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(04):1383.
[2]韩俊峰,阮志华,张蓓,等.HBV整合引起HepG2细胞microRNA表达谱变化分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2128.
　HAN Jun-feng,RUAN Zhi-hua,ZHANG Bei,et al.Differential microRNA expression profile of HepG2 cells induced by HBV integration[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):2128.
[3]卢荣鑫,钟前进.左向右分流型肺动脉高压大鼠肺组织中microRNA的表达谱及初步分析[J].陆军军医大学学报(原第三军医大学学报),2012,34(06):504.
　Lu Rongxin,Zhong Qianjin.A preliminary analysis of microRNA expression profiles in pulmonary tissues of rats with left-to-right shunt pulmonary hypertension[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(04):504.
[4]杨先涛,张祯祯,詹学,等.肺结核患儿外周血单个核细胞中microRNA表达的初步研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(19):2051.
　Yang Xiantao,Zhang Zhenzhen,Zhan Xue,et al.microRNA expression in peripheral blood mononuclear cells from pulmonary tuberculosis children[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(04):2051.
[5]袁世梅,李欢,杨敏,等.HIFU通过下调miR-181a进而促进ICAM-1表达增强小鼠抗肿瘤免疫[J].陆军军医大学学报(原第三军医大学学报),2015,37(01):16.
　Yuan Shimei,Li Huan,Yang Min,et al.Role and mechanism of miR-181a in high-intensity focused ultrasound-induced anti-tumor immunity in mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(04):16.
[6]周华,王园园,林子晶,等.miR-382-5p通过Akt/mTOR信号通路影响乳腺癌肿瘤相关巨噬细胞极化[J].陆军军医大学学报(原第三军医大学学报),2021,43(14):1358.
　ZHOU Hua,WANG Yuanyuan,LIN Zijing,et al.miR-382-5p affects polarization of tumor-associated macrophages in breast cancer through Akt/mTOR signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2021,43(04):1358.

更新日期/Last Update: 2014-02-12