[1]吴剑,张敏,戴天阳,等.siRNA干扰 MACC1基因对食管癌细胞TE-1的生物学影响[J].第三军医大学学报,2014,36(05):442-445.
 Wu Jian,Zhang Min,Dai Tianyang,et al.Biological effects of MACC1 siRNA interference on esophageal carcinoma TE-1 cells[J].J Third Mil Med Univ,2014,36(05):442-445.
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siRNA干扰 MACC1基因对食管癌细胞TE-1的生物学影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第05期
页码:
442-445
栏目:
论著
出版日期:
2014-03-15

文章信息/Info

Title:
Biological effects of MACC1 siRNA interference on esophageal carcinoma TE-1 cells
作者:
吴剑张敏戴天阳任德莲
泸州医学院免疫学教研室
Author(s):
Wu Jian Zhang Min Dai Tianyang Ren Delian
Department of Immunology, Luzhou Medical College, Luzhou, Sichuan Province, 646000, China
关键词:
食管鳞癌结肠癌转移相关基因1RNA小分子干扰细胞增殖细胞迁移肿瘤侵袭
Keywords:
esophageal squamous cell carcinomametastasis-associated in colon cancer 1RNA small interfering cell proliferation cell movement neoplasm invasiveness
分类号:
R394.3; R730.23; R735.1
文献标志码:
A
摘要:
目的      通过siRNA干扰沉默结肠癌转移相关基因1(metastasis-associated in colon cancer 1,MACC1),观察其对食管癌细胞株TE-1增殖、迁移及侵袭能力的影响。      方法        通过化学合成的特异性siRNA与阳离子脂质体LipofectamineTM 2000形成复合体,转染食管癌细胞株TE-1,应用qRT-PCR、Western blot检测转染后MACC1基因和蛋白表达水平;MTT法检测细胞生长增殖水平;划痕实验、Transwell实验检测细胞株干扰前后迁移侵袭能力的改变。      结果      siRNA-MACC1转染TE-1细胞后,与空白组比较,干扰组MACC1基因和蛋白的表达水平明显降低,分别降低71%和87%;MTT实验观察1~7 d,干扰组较空白组、阴性对照组增殖速度明显降低(P<0.05),第4天后效果更明显;划痕实验结果显示干扰组迁移划痕处的细胞(10.6±2.2)明显少于空白组(68.2±2.5),差异具有统计学意义(P<0.05),各对照组间无统计学差异(P>0.05);Transwell实验结果显示干扰组穿过膜的TE-1细胞数目(4.2±1.9)较空白组(58.4±7.4)明显减少(P<0.05),各对照组间无统计学差异(P>0.05),表明干扰后TE-1细胞的迁移侵袭能力显著降低。      结论      siRNA-MACC1干扰导致TE-1细胞MACC1基因及蛋白表达水平明显下调;MACC1基因下调后,食管癌TE-1细胞的增殖、迁移、侵袭能力均明显降低,说明MACC1基因具有促进食管癌细胞增殖、迁移、侵袭的能力。
Abstract:
Objective        To determine the effects of siRNA interfering metastasis-associated in colon cancer 1 (MACC1) on the extracorporeal proliferation, migration and invasion of the esophageal squamous cell carcinoma (ESCC) cell line TE-1.       Methods        The special MACC1-siRNA sequence was chemically synthesize, and then transfected into the TE-1 cells with lipofectamineTM 2000. RT-quantity PCR and Western blotting were used to detect the mRNA and protein level of MACC1 in untreated TE-1 cells, lipo2000-treated cells (mock), siRNA-nc transfected cells, and siRNA-MACC1 transfected cells. MTT assay was used to detect the proliferation of TE-1 cells. Scratching test and Transwell chamber assay were evaluated the migration of the cells.       Results      Compared with the untreated cell, mock, and siRNA-nc cells, the expression of MACC1 was obviously decreased by 71% at mRNA and 87% at protein levels. In 7 dafter transfection, MTT assay indicated that the proliferation of the transfected TE-1 cells was significantly inhibited compared with the other 3 types of cells (P<0.05), especially from the fourth day. Scratching test showed that there were obviously less migrated cells from the siRNA-MACC1 group than those from the control (10.6±2.2 vs 68.2±2.5, P<0.05). Transwell assay also showed the invasion ability of TE-1 cells transfected with siRNA-Macc1 was lesser than the control cells (4.2±1.92 vs 58.4±7.44, P<0.05). But no significant difference was seen among the untreated cell, mock, and siRNA-nc cells.       Conclusion       siRNA interference of MACC1 obviously down-regulates the molecule at mRNA and protein levels in TE-1 cells, and results in significant inhibition in the proliferation, migration and invasion of the cells. Therefore, MACC1 might be involved in the growth, migration and invasion of ESCC.

参考文献/References:

吴剑, 张敏, 戴天阳, 等. siRNA干扰 MACC1基因对食管癌细胞TE-1的生物学影响 [J].第三军医大学学报,2014,36(5):442-445.

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更新日期/Last Update: 2014-03-03