[1]张萌,彭利,乔治斌,等.罗格列酮激活p38MAPK通路调控p53及p21影响人肝癌HepG2细胞周期[J].第三军医大学学报,2014,36(03):240-243.
 Zhang Meng,Peng Li,Qiao Zhibin,et al.Rosiglitazone induces cell cycle arrest in hepatocellular carcinoma cell line HepG2 via p38MAPK/p53/p21 pathway[J].J Third Mil Med Univ,2014,36(03):240-243.
点击复制

罗格列酮激活p38MAPK通路调控p53及p21影响人肝癌HepG2细胞周期(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
36卷
期数:
2014年第03期
页码:
240-243
栏目:
论著
出版日期:
2014-02-15

文章信息/Info

Title:
Rosiglitazone induces cell cycle arrest in hepatocellular carcinoma cell line HepG2 via p38MAPK/p53/p21 pathway
作者:
张萌彭利乔治斌何宏涛周烨
河北医科大学第四医院肝胆外科
Author(s):
Zhang Meng Peng Li Qiao Zhibin He Hongtao Zhou Ye
Department of Hepatobiliary Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, Hebei Province, 050011, China
关键词:
罗格列酮肝细胞癌细胞周期p38丝裂原活化蛋白激酶 p53p21
Keywords:
rosiglitazonehepatocellular carcinomacell cyclep38MAPKp53p21
分类号:
R73-361; R730.23; R735.7
文献标志码:
A
摘要:
目的      研究p38丝裂原活化蛋白激酶(p38MAPK)通路及相关蛋白在罗格列酮引发的人肝癌HepG2细胞周期阻滞过程中的作用。      方法       MTT法检测罗格列酮对人肝癌HepG2细胞增殖的影响,流式细胞术检测细胞周期分布,Western blot检测p38MAPK通路相关蛋白的表达变化。      结果       罗格列酮可抑制HepG2细胞的增殖,并引发G0/G1期阻滞(P<0.05)。Western blot检测结果显示罗格列酮可激活p38MAPK通路,上调HepG2细胞中磷酸化p53蛋白及p21蛋白的表达水平(P<0.05);而ERK1/2及JNK的磷酸化程度没有明显变化。p38MAPK通路抑制剂SB203580可明显减弱罗格列酮对HepG2细胞的增殖抑制及周期阻滞作用;并且SB203580可部分逆转由罗格列酮引发的磷酸化p53及p21蛋白的表达变化。      结论       罗格列酮可通过激活p38MAPK通路引发人肝癌HepG2细胞周期阻滞,其机制可能与p38MAPK通路激活后参与对磷酸化p53蛋白及p21蛋白的调控有关。
Abstract:
Objective       To explore the role of p38MAPK signaling pathway in rosiglitazone (ROZ)-induced cell cycle arrest in human hepatocellular carcinoma cell line HepG2.       Methods       HepG2 cells were treated with different concentrations of ROZ. The proliferation inhibitory rates were analyzed by MTT assay. Cell cycle distributions were detected by flow cytometry (FCM). Western blotting was used to detect the expression of proteins related to p38MAPK signaling pathway.       Results       ROZ significantly inhibited proliferation of HepG2 cells, and induced an increase in the percentage of G0/G1 phase cells and a decrease in the percentage of S phase cells accompanied by the change in DNA ploidy (P<0.05). ROZ increased p38MAPK phosphorylation but not ERK1/2 or JNK phosphorylation, and up-regulated the protein expression levels of p-p53 and p21 in HepG2 cells (P<0.05). In addition, the ROZ-induced cell proliferation inhibition and cell cycle arrest were partly blocked by p38MAPK inhibitor SB203580, as well as the changes of p-p53 and p21 proteins.       Conclusion       ROZ inhibits cell proliferation and induces cell cycle arrest in G0/G1 stage of HepG2 cells by the activation of p38MAPK pathway, which may be mediated by the p38MAPK/p53/p21 signaling axis.

参考文献/References:

张萌, 彭利, 乔治斌, 等. 罗格列酮激活p38MAPK通路调控p53及p21影响人肝癌HepG2细胞周期[J].第三军医大学学报,2014,36(3):240-243.

相似文献/References:

[1]宋春丽,任吉华,张祯祯,等.SIRT1基因沉默诱导肝癌细胞老化及其机制[J].第三军医大学学报,2012,34(19):1929.
 Song Chunli,Ren Jihua,Zhang Zhenzhen,et al.Silent information regulator 1 gene induces aging of hepatocellular carcinoma cells via p53/p21 pathway[J].J Third Mil Med Univ,2012,34(03):1929.
[2]李建国,江小杰.原发性肝癌RUNX3基因启动子区甲基化及其意义[J].第三军医大学学报,2012,34(19):1933.
 Li Jianguo,Jiang Xiaojie.Methylation of RUNX3 gene promoter in HCC and its significance[J].J Third Mil Med Univ,2012,34(03):1933.
[3]左国华,梁平,李洪艳.肝细胞癌免疫磁珠的制备及鉴定[J].第三军医大学学报,2005,27(16):1700.
[4]蒙颖,徐芳,王志禄,等.罗格列酮对泡沫细胞中胆固醇贮存与运输相关蛋白ACAT-1、ABCA-1表达的影响[J].第三军医大学学报,2012,34(22):2288.
 Meng Ying,Xu Fang,Wang Zhilu,et al.Effect of rosiglitazone on expression of acyl-coenzyme A cholesterol acyltransferase 1 and ATP-binding cassette transporter A1 in foam cells[J].J Third Mil Med Univ,2012,34(03):2288.
[5]张小丽,高建,贾茜,等.肝癌干细胞样细胞的分离及其耐药性受PI3K/Akt通路调节[J].第三军医大学学报,2013,35(02):99.
 Zhang Xiaoli,Gao Jian,Jia Qian,et al.Isolation of HCC cancer stem-like cells and chemo-resistance mediated by PI3K/Akt pathway[J].J Third Mil Med Univ,2013,35(03):99.
[6]潘光栋,严律南,王新平,等.RNA干扰逆转肝细胞癌多药耐药[J].第三军医大学学报,2008,30(01):35.
 PAN Guang-dong,YAN Lu-nan,WANG Xin-ping,et al.Reversal of multidrug resistance of hepatocellular carcinoma by siRNA/mdr1[J].J Third Mil Med Univ,2008,30(03):35.
[7]谢斌,唐春,吴刚,等.c-Met、MMP-2、MMP-9在肝细胞癌中的表达及临床意义[J].第三军医大学学报,2008,30(12):1140.
 XIE Bin,TANG Chun,WU Gang,et al.Expressions and clinical significances of c-Met, MMP-2, MMP-9 in metastasis of human hepatocellular carcinoma: report of 47 cases[J].J Third Mil Med Univ,2008,30(03):1140.
[8]廖翠薇,邹利光,卫静,等.实验性肝细胞癌SPIO增强MRI及其病理学对照研究[J].第三军医大学学报,2006,28(01):38.
[9]李静,沈宜,杨麟,等.小鼠肝癌细胞(H22)源外泌体的体内抗肝癌作用研究[J].第三军医大学学报,2008,30(19):1836.
 LI Jing,SHEN Yi,YANG Lin,et al.Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22[J].J Third Mil Med Univ,2008,30(03):1836.
[10]白晓苏,张素华,丘彦,等.罗格列酮对多囊卵巢综合征患者胰岛素敏感性和β细胞功能之改善[J].第三军医大学学报,2006,28(18):1894.

更新日期/Last Update: 2014-01-24