[1]姚一搏,许民辉,耿明英,等.不同拷贝数CArG/5HRE+HSV-TK系统辅助伽玛刀杀伤C6胶质瘤的初步研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(23):2547-2550.
 Yao Yibo,Xu Minhui,Geng Mingying,et al.Cytotoxic effect of gamma knife assisted with different copy numbers of CArG/5HRE+HSV-TK system on C6 glioma cells[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(23):2547-2550.
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不同拷贝数CArG/5HRE+HSV-TK系统辅助伽玛刀杀伤C6胶质瘤的初步研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第23期
页码:
2547-2550
栏目:
论著
出版日期:
2013-12-15

文章信息/Info

Title:
Cytotoxic effect of gamma knife assisted with different copy numbers of CArG/5HRE+HSV-TK system on C6 glioma cells
作者:
姚一搏许民辉耿明英谭勇李飞鹏沈光建
安徽省临泉县人民医院神经外科;第三军医大学大坪医院野战外科研究所:神经外科,核磁共振室
Author(s):
Yao Yibo Xu Minhui Geng Mingying Tan Yong Li Feipeng Shen Guangjian
Department of Neurosurgery, People’s Hospital of Linquan County, Linquan, Anhui Province, 236400; Department of Neurosurgery, MRI Room, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
CArG/HREHSV-TK胶质瘤伽玛刀
Keywords:
CArG/HREherpessimplex virus-thymidine kinasegliomaGamma knife
分类号:
R73-362; R730.56; R730.264
文献标志码:
A
摘要:
目的      研究不同拷贝数CArG/5HRE+HSV-TK系统辅助伽玛刀杀伤C6胶质瘤效果,寻求伽玛刀条件下对胶质瘤杀伤更有效的适宜拷贝数CArG/5HRE+HSV-TK系统。      方法      在Wistar大鼠颅内C6胶质瘤模型的基础上,构建不同拷贝数CArG/5HRE+HSV-TK质粒(空质粒转染+伽玛刀照射组、HSV-TK +伽玛刀照射组、4CArG/5HRE启动子+伽玛刀照射组、9CArG/5HRE启动子+伽玛刀照射组、12CArG/5HRE启动子+伽玛刀照射组),通过脂质体转染到大鼠颅内胶质瘤组织中,对瘤组织伽玛刀放射外科治疗后取大鼠肿瘤组织。通过对瘤组织常规病理、瘤体细胞凋亡、HSV-TK mRNA及蛋白表达的观察,确定不同拷贝数CArG/5HRE+HSV-TK系统辅助伽玛刀诱导HSV-TK表达及对C6胶质瘤杀伤效果。      结果       各组肿瘤细胞均可见凋亡、破坏,CArG/HRE双启动子+伽马刀照射组更为明显(P<0.05),尤以9CArG/5HRE启动子+伽马刀照射组最为突出,凋亡率为(0.71±0.12)% (P<0.05);CArG/HRE双启动子+伽马刀照射组中HSV-TK mRNA及蛋白表达较无启动子组明显增加(P<0.05),9CArG/5HRE启动子+伽马刀照射组最为明显,HSV-TK mRNA的相对表达量为(0.84±0.09,P<0.05)。      结论      在SRS治疗条件下,CArG/HRE双启动子能促进目的基因HSV-TK的表达,增加对C6胶质瘤细胞的杀伤,其中,9CArG/5HRE双启动子的作用最明显。
Abstract:
Objective      To determine the cytotoxic effect of gamma knife assisted with different copy numbers of CArG/5HRE+ herpessimplex virus-thymidine kinase (HSV-TK) system on C6 glioma cells.       Methods      Intracranial C6 glioma model was established in Wister rats. The recombinant plasmids with different copy numbers of CArG/5HRE and suicide gene HSV-TK were artificially constructed. These different recombinant plasmids were transfected into C6 glioma rats with GCV injected abdominally afterward. After Gamma knife, tumor tissue of rats was collected, and then the killing effect of the tumor was decided by conventional pathology, tumor cell apoptosis and the expression of HSV-TK gene.       Results      The destruction and apoptosis of tumor cells were observed in all group, and more obvious in the groups containing CArG/HRE promoter (P<0.05), especially in 9CArG/5HRE group (P<0.05). The mRNA and protein expression of HSV-TK was increased significantly in groups containing CArG/HRE promoter (P<0.05), especially in 9CArG/5HRE group (P<0.05).       Conclusion      Under stereotactic radiosurgery (SRS) treatment, CArG/HRE double promoter significantly promotes the expression of target gene HSV-TK, and enhances the killing effect to C6 glioma cells. The effect of 9CArG/5HRE double promoter is identified as the most significant.

相似文献/References:

[1]罗顺文,叶钢,张荣贵,等.全反式维甲酸增强HSV-TK/GCV对BIU-87细胞旁观者效应的观察[J].陆军军医大学学报(原第三军医大学学报),2005,27(05):385.

更新日期/Last Update: 2013-12-06