[1]许颖,李晓庆,王丹,等.嗜肺军团菌LP1株MompS抗原表位的预测与验证[J].第三军医大学学报,2013,35(16):1685-1687.
 Xu Ying,Li Xiaoqing,Wang Dan,et al.Prediction and confirmation of B cell epitope for MompS antigen of Legionella pneumophila serogroup 1[J].J Third Mil Med Univ,2013,35(16):1685-1687.
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嗜肺军团菌LP1株MompS抗原表位的预测与验证(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第16期
页码:
1685-1687
栏目:
论著
出版日期:
2013-08-30

文章信息/Info

Title:
Prediction and confirmation of B cell epitope for MompS antigen of Legionella pneumophila serogroup 1
作者:
许颖李晓庆王丹王建常晓松
成都医学院第一附属医院检验科;四川出入境检验检疫局
Author(s):
Xu Ying Li XiaoqingWang DanWang Jian Chang Xiaosong
Department of Clinical Laboratory, First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan Province, 610500; Sichuan Provincial Bureau of Entry-exit Inspection and Quarantine, Chengdu, Sichuan Province, 610041, China
关键词:
嗜肺军团菌B抗原表位多肽疫苗
Keywords:
Legionella pneumophila B cell epitope peptide vaccine
分类号:
R378.99;R394.3
文献标志码:
A
摘要:
目的      探讨以嗜肺军团菌LP1株MompS抗原表位为基础的合成肽的免疫作用。      方法      在GenBank中检索LP1株MompS基因信息,利用在线生物信息学软件,结合DNA Star软件的蛋白质二级结构预测理论方案,预测其B表位抗原位点,设计合成具有LP1血清型特异性氨基酸序列的多肽,通过肽结合实验和动物免疫学效果评价检测合成肽对LP1免疫血清的结合能力。      结果      筛选得到3条多肽GP-15、NY-17、AA-19,多肽肽结合实验表明GP-15D(450)反应值显著高于NY-17和AA-19片段(P<0.05),动物免疫学效果评价显示其各时间段的IgG抗体几何平均滴度显著高于NY-17和AA-19(P<0.05)。      结论      证实3条多肽均具备了MompS表位属性,GP-15肽结合实验和动物免疫效果评价均提示其可作为嗜肺军团菌多肽疫苗的候选肽段。
Abstract:
Objective      To explore the immune function of antigen epitope peptides based on MompS antigen of Legionella pneumophila serogroup 1(LP1).       Methods      The genetic information of LP1 MompS antigen was retrieved in GenBank by using bioinformatics software online. Combined with the prediction scheme theory of protein secondary structure by DNA Star, the B epitope antigen sites were predicted, and then the polypeptides which have the specific amino acid sequence of LP1 were synthesized. The binding ability of the obtained polypeptides to LP1 was detected by peptides binding assay and animal immunology effect assessment.       Results      Three obtained peptides, GP-15, NY-17 and AA-19 were screened out, and the GP-15 peptide had the best immunogenic ability among 3 peptides in the peptides binding assay (P<0.05) and animal immuno-logy effect assessment (P<0.05).       Conclusion      All of the 3 peptides are MompS antigen epitope peptides, and the GP-15 can be used to construct vaccine of Legionella pneumophila.

参考文献/References:

许颖, 李晓庆, 王丹, 等. 嗜肺军团菌LP1株MompS抗原表位的预测与验证[J].第三军医大学学报,2013,35(16):1685-1687.

更新日期/Last Update: 2013-08-02