[1]谭洪波,段小军,杨柳,等.透明软骨材料经脱细胞处理后细胞外基质成分变化的比较[J].第三军医大学学报,2013,35(19):2028-2032.
 Tan Hongbo,Duan Xiaojun,Yang Liu,et al.Alteration of extracellular matrix in decellularized articular cartilage treated by different technologies[J].J Third Mil Med Univ,2013,35(19):2028-2032.
点击复制

透明软骨材料经脱细胞处理后细胞外基质成分变化的比较(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第19期
页码:
2028-2032
栏目:
论著
出版日期:
2013-10-15

文章信息/Info

Title:
Alteration of extracellular matrix in decellularized articular cartilage treated by different technologies
作者:
谭洪波段小军杨柳徐永清丁晶杨军
第三军医大学西南医院关节外科;解放军昆明总医院全军骨科研究所
Author(s):
Tan Hongbo Duan Xiaojun Yang Liu Xu Yongqing Ding Jing Yang Jun
Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Institute of Orthopaedics, General Hospital of Kunming Military Command, Kunming, Yunnan Province, 650032, China
关键词:
组织工程软骨软骨细胞支架材料
Keywords:
tissue engineering cartilage chondrocyte scaffold
分类号:
R322.72; R329-33
文献标志码:
A
摘要:
目的      比较Triton X-100和十二烷基硫酸钠(SDS)试剂对人关节软骨脱细胞处理后细胞外基质成分的变化,指导筛选适宜的制备。       方法       首先制备人关节软骨微粒,再采用两种不同试剂分别作用于软骨细胞微粒,清洗和消化步骤相同,脱细胞完成后分别进行DNA、总氨基酸及总胶原和葡萄糖胺聚糖(GAG)含量检测,分为Triton X-100组和SDS组,并与对照组(正常软骨组织)进行基质成分的比较分析。       结果        Triton X-100组、SDS组和对照组经脱细胞处理后其GAG含量分别为(84.71±3.72)、(122.63±6.05)、(232.78±75.33)μg/mg,羟脯氨酸含量分别为(24.92±1.74)、(24.23±1.68)、(24.54±2.99)μg/mg,DNA含量分别为(0.242±0.065)、(0.225±0.057)、(5.679±1.873)μg/mg。       结论        Triton X-100和SDS两种试剂均能将软骨微粒中软骨细胞成分有效去除,胶原成分保留量在两组中无明显差异;但是应用SDS行脱细胞处理后能保留更多的GAG成分,更有利于维持软骨细胞功能,是较好的材料制备技术。
Abstract:
Objective      To compare the changes of extracellular matrix in decellularized articular cartilage treated by Triton X-100 and sodium dodecyl sulfate(SDS) to optimize the suitable preparation.       Methods      First, cartilage particles were prepared, then the two reagents was used to treat the cells of cartilage particles respectively, with the same experimental procedures of rinsing and digestion. After decellularization, the contents of DNA, amino acid, collagen and glycosaminoglycan  (GAG) were measured, and the results were compared with those of the normal cartilaginous tissue.       Results      After decellularization, the GAG contents were 84.71±3.72 and 122.63±6.05 μg/mg respectively in the extracellular matrix of articular cartilages treated by Triton X-100 and SDS, while that was 232.78±75.33 μg/mg in the extracellular matrix of normal cartilaginous cells. Hydroxyproline content was 24.92±1.74 and 24.23±1.68 μg/mg respectively after the decellularization, and that in the normal matrix was 24.54±2.99 μg/mg. DNA content was 0.242±0.065 and 0.225±0.057 μg/mg respectively, and that in the normal one was 5.679±1.873 μg/mg.       Conclusion      Cell components are removed effectively from cartilage particles by both Triton X-100 and SDS without obvious difference of collagen remains. While SDS reserves more GAG components and is more helpful to maintain the cartilage cells’ function. So SDS decellularization is a better preparative technique for tissue engineering.

参考文献/References:

谭洪波,段小军,杨柳,等. 透明软骨材料经脱细胞处理后细胞外基质成分变化的比较[J].第三军医大学学报,2013,35(19):2028-2032.

相似文献/References:

[1]樊东力,张一鸣.生物医用材料和组织工程技术在组织修复中的应用及进展[J].第三军医大学学报,2015,37(19 ):1909.
 Fan Dongli,Zhang Yiming.Application and progress of biomaterial and tissue engineering in tissue repair[J].J Third Mil Med Univ,2015,37(19):1909.
[2]王直兵,张瑗,张峡,等.移行结构化细胞-肌腱复合物重建韧带-骨连接体的实验研究[J].第三军医大学学报,2015,37(19 ):1936.
 Wang Zhibing,Zhang Yuan,Zhang Xia,et al.Reconstruction of ligament-bone interface by a tissue-engineered cell-tendon complex with characteristics of transitional architecture and its efficacy in rabbits[J].J Third Mil Med Univ,2015,37(19):1936.
[3]罗飞,许建中.基因增强的骨组织工程研究进展[J].第三军医大学学报,2005,27(16):1702.
[4]李强,何清义,许建中.VEGF促进骨组织工程血管化的研究进展[J].第三军医大学学报,2005,27(16):1704.
[5]戴书华,邹利光,廖翠薇,等.腰椎椎体后缘软骨结节的影像学诊断[J].第三军医大学学报,2006,28(01):24.
[6]刘雷,李起鸿,唐康来,等.异种脱蛋白骨的生物相容性研究[J].第三军医大学学报,2006,28(22):2227.
[7]董伟强,白波,陈艺,等.基因重组人生长素对关节软骨缺损修复作用的实验研究[J].第三军医大学学报,2006,28(13):1417.
[8]吕仁发,周强,许建中,等.双相接种法体外构建组织工程骨[J].第三军医大学学报,2005,27(16):1656.
[9]秦辉,许建中,王序全,等.双相接种技术构建组织工程骨的异位成骨观察[J].第三军医大学学报,2005,27(16):1660.
[10]吕仁发,周强,许建中,等.循环灌注式三维组织培养装置的设计及其性能测试[J].第三军医大学学报,2005,27(16):1663.
[11]崔运利,王富友,谭洪波,等.应用高浓度Ⅱ型胶原蛋白构建组织工程软骨支架[J].第三军医大学学报,2011,33(14):1511.
 Cui Yunli,Wang Fuyou,Tan Hongbo,et al.Construction of scaffold for treatment of articular cartilage defect using high-concentration collagen Ⅱ[J].J Third Mil Med Univ,2011,33(19):1511.
[12]周强,李起鸿,杨柳,等.骺板软骨细胞复合生物凝胶体外培养生成软骨组织的实验研究[J].第三军医大学学报,2002,24(05):0.[doi:10.16016/j.1000-5404.2002.05.027 ]
 ZHOU Qiang,LI Qi-hong,YANG Liu,et al.[J].J Third Mil Med Univ,2002,24(19):0.[doi:10.16016/j.1000-5404.2002.05.027 ]
[13]周强,杨柳,戴刚,等.骺板组织工程的研究进展[J].第三军医大学学报,2003,25(02):0.[doi:10.16016/j.1000-5404.2003.02.034 ]

更新日期/Last Update: 2013-09-30