[1]刘雪萍,贺斌峰,陈华萍,等.新型纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸对肺动脉平滑肌细胞增殖和凋亡的作用[J].第三军医大学学报,2013,35(09):846-849.
 Liu Xueping,He Binfeng,Chen Huaping,et al.Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells[J].J Third Mil Med Univ,2013,35(09):846-849.
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新型纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸对肺动脉平滑肌细胞增殖和凋亡的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第09期
页码:
846-849
栏目:
论著
出版日期:
2013-05-15

文章信息/Info

Title:
Effect of a novel nanosystem of Ac-αCD encapsulating Bcl-xl antisense oligonucleotide on proliferation and apoptosis in pulmonary arterial smooth muscle cells
作者:
刘雪萍贺斌峰陈华萍孙欢杨俊俊魏征华窦寅王关嵩
第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室;第三军医大学药学院药剂学教研室
Author(s):
Liu Xueping He Binfeng Chen Huaping Sun Huan Yang Junjun Wei Zhenghua Dou Yin Wang Guansong
Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037; Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, Chongqing, 400038, China
关键词:
纳米载体Bcl-xl反义寡核苷酸肺动脉平滑肌细胞
Keywords:
nanosystems Bcl-xl antisense oligonucleotide pulmonary arterial smooth muscle cells
分类号:
R322.121; R329.28; R394.2
文献标志码:
A
摘要:
目的   研究纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸(antisense oligonucleotide, ASON)对大鼠肺动脉平滑肌细胞(rat pulmonary arterial smooth muscle cells,RPASMCs)增殖和凋亡作用。   方法   设计合成5′端标记Cy3的Bcl-xl ASON,由纳米载体Ac-αCD携带。实验分3组:纳米载体携带的Bcl-xl ASON组(ASON-NPs组)、单纯纳米载体组(NPs组)和空白对照组,分别使用纳米载体Ac-αCD携带的Bcl-xl ASON、纳米载体Ac-αCD和培养液处理RPASMCs 48 h,激光共聚焦显微镜观察RPASMCs对纳米载体携带的Bcl-xl ASON的摄取情况;RT-PCR、Western blot检测Bcl-xl的mRNA和蛋白表达;MTT检测处理后细胞的增殖抑制率;流式细胞仪检测细胞凋亡率。   结果   激光共聚焦显微镜下可见ASON-NPs组细胞质内大量呈颗粒状均匀分布的红色荧光物质,空白对照组和NPs组细胞细胞质内未见红色荧光物质;ASON-NPs组处理的RPASMCs的Bcl-xl mRNA和蛋白表达显著低于空白对照组和NPs组(P<0.05); ASODN-NPs组、NPs组、空白对照组细胞抑制率分别为:(53.61±3.02)%、(6.30±1.90)%、(1.40±0.62)%,凋亡率分别为:(53.04±2.09)%、(10.98±2.03)%、(2.19±0.11)%、ASON-NPs组和NPs组细胞抑制率、凋亡率均显著高于空白对照组 (P<0.01),ASON-NPs组均显著高于NPs组 (P<0.01)。   结论   纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸能被RPASMCs有效摄取,从而抑制其增殖,促进凋亡。
Abstract:
Objective    To determine the effect of an Ac-αCD nanosystem encapsulating Bcl-xl antisense oligonucleotide (ASON) on the proliferation and apoptosis in pulmonary arterial smooth muscle cells.    Methods    Bcl-xl ASON that had been hallmarked with the Cy3 in 5′-end was synthesized, and then encapsulated into the nanosystem Ac-αCD. Primarily cultured SD rat pulmonary arterial smooth muscle cells were treated by Ac-αCD-Bcl-xl ASON or Ac-αCD for 48 h, and the cells without nanosystem served as control. Confocal microscopy was employed to observe the taking of the nanosystem by the cells. Expression of Bcl-xl at mRNA and protein levels, grow inhibitory rate and apoptotic rate were detected by RT-PCR and Western blotting, MTT assay and flow cytometry, respectively.    Results    There were a great deal of brilliantly red-fluorescent granules distributed evenly in the cytoplasm in the cells treated by Ac-αCD-Bcl-xl ASON. No such red-fluorescent granule was seen in the other 2 kinds of cells. The expression of Bcl-xl at mRNA and protein levels were significantly lower in the cells treated by Ac-αCD-Bcl-xl ASON (P<0.05). Cell growth inhibitory rate was (53.61±3.02)% in Ac-αCD-Bcl-xl ASON, (6.30±1.90)% in Ac-αCD and (1.40±0.62)% in control cells. Cell apoptotic rate was (53.04±2.09)% in Ac-αCD-Bcl-xl ASON, (10.98±2.03)% in Ac-αCD and (2.19±0.11)% in control cells, with the former 2 kinds of cells significantly higher than control (P<0.01), so Ac-αCD-Bcl-xl ASON cells than Ac-αCD cells (P<0.01).    Conclusion    Our Ac-αCD nanosystem encapsulating Bcl-xl ASON is effectively taken by rat pulmonary arterial smooth muscle cells, and then inhibits cell growth and induces cell apoptosis.

参考文献/References:

刘雪萍, 贺斌峰, 陈华萍, 等. 新型纳米载体Ac-αCD携带的Bcl-xl反义寡核苷酸对肺动脉平滑肌细胞增殖和凋亡的作用[J].第三军医大学学报,2013,35(9):846-849.

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更新日期/Last Update: 2013-05-03