[1]陈书梅,杨淑敏,张文龙,等.TNF-α促进HepG2肝细胞脂质积聚及其机制的初步研究[J].第三军医大学学报,2013,35(11):1088-1092.
 Chen Shumei,Yang Shumin,Zhang Wenlong,et al.TNF-α promotes HepG2 hepatocytes lipid accumulation[J].J Third Mil Med Univ,2013,35(11):1088-1092.
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TNF-α促进HepG2肝细胞脂质积聚及其机制的初步研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第11期
页码:
1088-1092
栏目:
论著
出版日期:
2013-06-15

文章信息/Info

Title:
TNF-α promotes HepG2 hepatocytes lipid accumulation
作者:
陈书梅杨淑敏张文龙吕琼叶鹏高茹菲梅玫汪志红李启富
重庆医科大学附属第一医院内分泌科
Author(s):
Chen Shumei Yang Shumin Zhang Wenlong Lyu Qiong Ye Peng Gao Rufei Mei Mei Wang Zhihong Li Qifu
Department of Endocrinology, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
TNF-α软脂酸SREBP-1HepG2肝细胞脂质积聚
Keywords:
TNF-αpalmitateSREBP-1HepG2 hepatocyteslipid accumulation
分类号:
R322.47;R341.32;R349.15
文献标志码:
A
摘要:
目的      探讨肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)是否能够促进肝细胞脂质积聚,并对其机制进行初步探讨。      方法          将HepG2肝细胞分为空白对照组、单纯TNF-α组(TNF-α 2 ng/mL或20 ng/mL)、软脂酸组(软脂酸0.08 mmol/L或0.2 mmol/L)及联合组(TNF-α 2 ng/mL联合软脂酸0.08 mmol/L、TNF-α 2 ng/mL联合软脂酸 0.2 mmol/L、TNF-α 20 ng/mL联合软脂酸 0.08 mmol/L、TNF-α 20 ng/mL联合软脂酸 0.2 mmol/L),处理24 h,应用化学酶促-比色法定量检测细胞内TG含量。进一步选取TNF-α 20 ng/mL和软脂酸0.08 mmol/L,通过油红O染色观察HepG2细胞内脂质积聚情况;实时荧光定量PCR和Western blot检测HepG2细胞SREBP-1、FAS、ACCα的表达水平。      结果          ①单纯TNF-α组TG含量[TNF-α 2 ng/mL组(0.344±0.093)μg/μg、TNF-α 20 ng/mL组(0.329±0.068)μg/μg]分别较空白对照组[(0.192±0.048)μg/μg]显著升高(P<0.05);联合组[TNF-α 2 ng/mL联合软脂酸 0.08 mmol/L组(0.451±0.096)μg/μg、TNF-α 2 ng/mL联合软脂酸 0.2 mmol/L组(0.821±0.257)μg/μg、TNF-α 20 ng/mL联合软脂酸 0.08 mmol/L组(1.032±0.286)μg/μg、TNF-α 20 ng/mL联合软脂酸 0.2 mmol/L组(2.134±1.049)μg/μg]分别较软脂酸组[软脂酸 0.08 mmol/L组(0.247±0.069)μg/μg、软脂酸0.2 mmol/L组(0.341±0.031)μg/μg]显著升高(P<0.05);②油红O染色进一步显示,TNF-α促进肝细胞内脂质积聚。③实时荧光定量PCR和Western blot检测结果显示单纯TNF-α组与空白对照组相比,HepG2细胞SREBP-1、FAS、ACCα的表达均增加(P<0.05);联合组与软脂酸组相比,肝细胞内SREBP-1、FAS、ACCα的表达水平明显上调(P<0.05)。      结论      TNF-α促进HepG2肝细胞内脂质积聚,增加SREBP-1、FAS、ACCα的表达。
Abstract:
Objective        To determine the effect of tumor necrosis factor-α (TNF-α) on lipid accumulation in HepG2 cells and its underlying possible mechanism.       Methods        HepG2 cells were treated with TNF-α (2 or 20 ng/mL), palmitate (PA, 0.08 or 0.2 mmol/L), and TNF-α plus palmitate (combination of the 2 doses of 2 agents) for 24 h, respectively. The intracellular triglyceride (TG) was measured by enzymatic colorimetric method. Then TNF-α of 20 ng/mL and palmitate of 0.08 mmol/L was chosen for the further experiment. Lipid accumulation in the HepG2 cells was observed with Oil Red O staining. Real-time PCR and Western blot analysis were used to detect the expression of SREBP-1, FAS and ACCα at mRNA and protein levels.       Results        TG level was significantly higher in TNF-α treated cells (0.344±0.093 and 0.329±0.068 μg/μg for the doses of 2 and 20 ng/mL) than in control cells (0.192±0.048 μg/μg, P<0.05). And that of the combination treatment cells (TNF-α 2 ng/mL plus PA 0.08 mmol/L: 0.451±0.096, TNF-α 2 ng/mL plus PA 0.2 mmol/L: 0.821±0.257, TNF-α 20 ng/mL plus PA 0.08 mmol/L: 1.032±0.286, TNF-α 20 ng/mL plus PA 0.2 mmol/L: 2.134±1.049 μg/μg) was significant higher than the cells treated by PA alone (PA 0.08 mmol/L: 0.247±0.069, PA 0.2 mmol/L: 0.341±0.031 μg/μg, all P<0.05). Oil red O staining also showed that TNF-α promoted lipid accumulation in HepG2 cells. The expression of SREBP-1, FAS and ACCα at mRNA and protein levels was significantly higher in TNF-α treatment and the TNF-α plus PA treatment cells than in control cells (P<0.05).       Conclusion        TNF-α promotes lipid accumulation, and enhances the expression of SREBP-1, FAS and ACCα in HepG2 hepatocytes.

参考文献/References:

陈书梅, 杨淑敏, 张文龙, 等. TNF-α促进HepG2肝细胞脂质积聚及其机制的初步研究[J].第三军医大学学报,2013,35(11):1088-1092.

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更新日期/Last Update: 2013-06-03