[1]陈侠,李磊,谢琳.壳聚糖纳米微粒荧光探针的制备及特性研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):992-995.
 Chen Xia,Li Lei,Xie Lin.Preparation and characterization of fluorescent chitosan nanoprobe[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(10):992-995.
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壳聚糖纳米微粒荧光探针的制备及特性研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第10期
页码:
992-995
栏目:
论著
出版日期:
2013-05-30

文章信息/Info

Title:
Preparation and characterization of fluorescent chitosan nanoprobe
作者:
陈侠李磊谢琳
第三军医大学大坪医院野战外科研究所:眼科,第一研究室
Author(s):
Chen Xia Li Lei Xie Lin
Department of Ophthalmology, State Key Laboratory of Trauma, Burns and Combined Injury, Department 1, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
纳米技术分子探针壳聚糖核酸适配子转化生子因子βⅡ型受体Tenon’s囊成纤维细胞
Keywords:
nanotechnology molecular probes chitosan aptamer TGF-β receptor Ⅱ human Tenon’s capsule fibroblasts
分类号:
R318.08;R394;R446.9
文献标志码:
A
摘要:
目的      制备出壳聚糖纳米微粒荧光探针,对其物理特性进行研究,并观察其靶向性及细胞毒性。      方法      使用离子交联法制备出壳聚糖纳米微粒荧光探针(chitosan-nanoparticles-fluorescein isothiocyanate,CS-NP-FITC)。Zeta粒径分析仪观察其粒径及表面电位情况;扫描电镜观察其形态特点;接着用靶向转化生子因子βⅡ型受体(TGF-β receptor Ⅱ,TβRⅡ)的核酸适配子S58标记CS-NP-FITC,作用于人Tenon’s囊成纤维细胞(human Tenon’s capsule fibroblasts,HTFs),观察其靶向结合特异性受体的情况;用LDH释放实验检测其细胞毒性。      结果      制备出的壳聚糖纳米微粒荧光探针CS-NP-FITC的平均粒径为274.6 nm,平均表面电位为+24.3 mV,扫描电镜下可观察到呈球形的微粒,分布均匀;激光共聚焦显微镜下观察到适配子S58标记的CS-NP-FITC能够与TβRⅡ特异性结合;LDH释放试验证实CS-NP-FITC的使用浓度在0.18 ~18 mg/mL时,细胞毒性很小,具有良好的生物相容性。      结论      壳聚糖纳米微粒荧光探针CS-NP-FITC经适当的适配子或抗体标记后具有靶向结合特异性受体的能力,且细胞毒性小,具有应用于生物成像中的潜力。
Abstract:
Objective      To synthesize a novel chitosan-nanoparticles-fluorescein isothiocyanate fluorescent nanoprobe (CS-NP-FITC), and to explore its physical properties, targeting ability and cytotoxicity.       Methods      CS-NP-FITC was synthesized by ionic cross-linking. A Zetasizer Nano ZS instrument was used to measure the diameter and zeta potential of CS-NP-FITC. The morphology of CS-NP-FITC was examined using a scanning electron microscope. CS-NP-FITC was labeled by aptamer S58 targeting transforming growth factor-β receptorⅡ (TβRⅡ) to test the targeting ability to specific receptors in human Tenon’s capsule fibroblasts. At last, the cytotoxicity of CS-NP-FITC was studied by LDH cytotoxicity assay.       Results      The mean diameter of CS-NP-FITC was 274.6 nm, and the zeta potential of CS-NP-FITC was +24.3 mV. The spherical particles were uniformly distributed shown by the scanning electron microscopy. CS-NP-FITC labeled by aptamer S58 could specifically bind to TβRⅡ under a laser scanning confocal microscope. LDH cytotoxicity assay showed that CS-NP-FITC at concentration between 0.18 mg/mL to 18 mg/mL had low cytotoxicity and fine biocompatibility.       Conclusion      Our study reveal that CS-NP-FITC labeled by suitable aptamer or antibody can specificly bind to target receptors. Due to its low cytotoxicity, CS-NP-FITC has the potential to be applied in bio-imaging.

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更新日期/Last Update: 2013-05-20