[1]李文迪,钟敏,刘友学,等.氯化钴后处理对新生鼠缺氧缺血性脑损伤学习记忆的作用[J].第三军医大学学报,2013,35(07):630-634.
 Li Wendi,Zhong Min,Liu Youxue,et al.Cobalt chloride post-conditioning improves spatial working memory in rats of neonatal hypoxic-ischemic encephalopathy[J].J Third Mil Med Univ,2013,35(07):630-634.
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氯化钴后处理对新生鼠缺氧缺血性脑损伤学习记忆的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第07期
页码:
630-634
栏目:
论著
出版日期:
2013-04-15

文章信息/Info

Title:
Cobalt chloride post-conditioning improves spatial working memory in rats of neonatal hypoxic-ischemic encephalopathy
作者:
李文迪钟敏刘友学陈洁程茜李廷玉代英
重庆医科大学附属儿童医院:儿童保健科,儿童营养研究中心,儿童发育疾病研究教育部重点实验室,儿科学重点实验室,重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地,神经内科
Author(s):
Li Wendi Zhong Min Liu Youxue Chen Jie Cheng Qian Li Tingyu Dai Ying
Department of Primary Child Healthcare, Children’s Nutritional Research Center, Key Laboratory of Developmental Diseases in Childhood of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorder, Department of Neurology, Children’s Hospital, Chongqing Medical University, Chongqing, 400014, China
关键词:
缺氧诱导因子氯化钴缺氧缺血性脑损伤空间学习记忆
Keywords:
hypoxia-inducible factor CoCl2 hypoxic-ischemic brain damage spatial working memory
分类号:
R338.64; R743.31; R914.3
文献标志码:
A
摘要:
目的      探讨氯化钴(cobalt chloride, CoCl2)后处理对缺氧缺血性脑损伤新生大鼠空间学习记忆的作用。      方法      生后7日龄SD大鼠66只,分为假手术组(n=16)、缺氧缺血组(HI组,n=18)、CoCl2即刻干预组(C1组,n=14)、CoCl2术后1 d干预组(C2组,n=18)。Western blot检测术后1、2、7 d脑组织HIF-1α蛋白的表达。大鼠生后7周行水迷宫观察CoCl2对大鼠空间学习记忆的影响。       结果       HI组和C1组HIF-1α蛋白在术后第1、2天增多,第7天则不能检测到HIF-1α蛋白。而C2组第7天仍能检测到HIF-1α蛋白表达。水迷宫实验显示C2组较HI组3~ 5 d时平均潜伏时间明显缩短,穿越原平台区域的时间增加,空间学习记忆能力部分恢复(P<0.05)。      结论      在新生鼠缺氧缺血性脑损伤时, CoCl2促进HIF-1α蛋白的持续表达,术后1 d干预可有效恢复大鼠的空间学习记忆能力。
Abstract:
Objective       To determine the effect of cobalt chloride (CoCl2) post-conditioning on the spatial working memory in neonatal rats with hypoxic-ischemic induced brain damage (HIBD).       Methods       Sixty-six SD rats aged 7 d were randomly divided into sham operation group (N, n=16), hypoxia-ischemia group (HI, n=18), CoCl2administration immediately after surgery group (C1, n=14) and CoCl2 administration 1 d after surgery group (C2, n=18). The left common carotid artery of rats were ligated after anesthesia followed by hypoxia. In the sham control group, the left common carotid artery was exposed but was not ligated or exposed to hypoxia. The brain tissues were harvested from the pups in each group at 1, 2 and 7 d after ligation. HIF-1α expression was detected by Western blot analysis. At age of 7 weeks, pups of each group were trained to do the Morris water maze test to evaluate their spatial learning and memory abilities.       Results       HIF-1α expression was up-regulated in HI, C1 and C2 groups at post operation days 1 and 2, then decreased, and even not detectable in the former 2 groups at postoperative day 7, but detectable in C2 group. Morris water maze training revealed that the rats in C2 group had significantly shortened average latency time at 3 to 5 d,  and improved long-term reference memory compared with that in HI group (P<0.05).       Conclusion       CoCl2 improves and maintains the expression of HIF-1α in newborn rats after hypoxic-ischemic induced brain damage. Post-conditioning with CoCl2 at 1 d after hypoxic-ischemic damage exerts neuroprotection and restores spatial working memory.

参考文献/References:

李文迪, 钟敏, 刘友学, 等. 氯化钴后处理对新生鼠缺氧缺血性脑损伤学习记忆的作用[J].第三军医大学学报,2013,35(7):630-634.

相似文献/References:

[1]林爽,李力,王云.缺氧诱导因子-1α及E-钙粘蛋白在宫颈癌中的表达及意义[J].第三军医大学学报,2005,27(10):1026.
[2]孙胜,高文祥,高钰琪.缺氧调节基因/产物参与CoCl2预处理对分化SH-SY5Y细胞缺氧损伤的保护[J].第三军医大学学报,2007,29(12):1168.
 SUN Sheng,GAO Wen-xiang,GAO Yu-qi.Roles of hypoxia regulated genes/protein in neuroprotection against hypoxia injury in differentiated SH-SY5Y cells preconditioned with CoCl2[J].J Third Mil Med Univ,2007,29(07):1168.

更新日期/Last Update: 2013-04-07