[1]唐永萍,杨阳,徐培,等.TO901317修复酒精致新生小鼠视网膜节细胞损伤的实验研究[J].第三军医大学学报,2013,35(05):376-380.
 Tang Yongping,Yang Yang,Xu Pei,et al.Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice[J].J Third Mil Med Univ,2013,35(05):376-380.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第05期
页码:
376-380
栏目:
论著
出版日期:
2013-03-15

文章信息/Info

Title:
Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice
作者:
唐永萍杨阳徐培徐海伟阴正勤
第三军医大学:西南医院全军眼科中心,视觉损伤与再生修复重庆市重点实验室,基础医学部发育生物学教研室
Author(s):
Tang Yongping Yang Yang Xu Pei Xu Haiwei Yin Zhengqin
Center of Ophthalmology, Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Southwest Hospital, Department of Developmental Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
关键词:
肝脏X受体视网膜神经节细胞层星形胶质细胞
Keywords:
liver X receptor retina ganglion cell layer astrocyte
分类号:
R394.2; R595.6; R774.1
文献标志码:
A
摘要:
目的      观察酒精暴露对新生小鼠视网膜结构的影响,以及肝脏X受体(LXRs)激动剂TO901317对酒精损伤小鼠视网膜的保护作用。      方法        将新生C57BL/6J小鼠分为对照组、酒精暴露组和激动剂TO预处理组,每组3只。采用HE染色检测各组小鼠视网膜大体结构改变,采用免疫组化染色检测各组小鼠视网膜NeuN阳性细胞数、GFAP表达变化。      结果        免疫组化染色发现LXRβ主要分布于新生小鼠视网膜节细胞层,HE染色发现酒精组新生小鼠视网膜节细胞数量较对照组显著减少(P<0.01),TO处理能显著翻转酒精暴露导致的视网膜节细胞层细胞数量减少(P<0.05);免疫组化染色发现酒精组小鼠眼球视网膜节细胞层NeuN阳性细胞数明显少于对照组(P<0.01),而TO预处理组较酒精组NeuN阳性细胞显著增多(P<0.01)。对各组进行GFAP免疫组化染色并采用光密度分析进行半定量分析,结果显示酒精组小鼠视网膜GFAP表达较对照组和TO预处理组显著增高(P<0.01)。      结论        LXRs受体激活可有效抑制酒精所致视网膜中星形胶质细胞的活化,挽救节细胞丢失。
Abstract:
Objective        To observe the effect of alcohol exposure on the retina of neonatal mice and the neuroprotective role of liver X receptor (LXR) agonist TO901317 (TO).       Methods        Mice were randomly divided into 3 groups, including a control group, an alcohol exposure group and a TO pretreatment group (n=3). Hematoxylin and eosin (HE) staining was used to observe the general structure of mouse retina, and immunochemistry was applied to detect NeuN-positive neurons and GFAP expression in the retina.       Results        LXRβ was mainly expressed in retinal ganglion cell layer of neonatal (postnatal day 6, P6) mice. HE staining showed a significant decrease in cell number in retinal ganglion cell layer of the alcohol exposure group compared with the control group (P<0.01), while TO could protect the ganglion cells from alcohol damage (P<0.05). Compared with the control group, NeuN-positive cells in the retinal ganglion cell layer of the alcohol exposure group significantly decreased (P<0.01), but the TO pretreatment group had more NeuN-positive cells than the alcohol exposure group (P<0.01). GFAP expression significantly increased in the retinal ganglion cell layer of the alcohol exposure group as compared with the control group and TO pretreatment group (P<0.01).       Conclusion        LXR agonist TO can inhibit alcohol-induced astrocyte activation in retina and rescue retinal ganglion cells in neonatal mice.

参考文献/References:

唐永萍, 杨阳, 徐培, 等. TO901317修复酒精致新生小鼠视网膜节细胞损伤的实验研究[J].第三军医大学学报,2013,35(5):376-380.

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更新日期/Last Update: 2013-03-05