[1]焦燕,邱倩,杨再兴,等.淋巴细胞微粒刺激AKT/Foxo1阻滞气道上皮细胞周期的研究[J].第三军医大学学报,2012,34(24):2498-2502.
 JiaoYan,Qiu Qian,Yang Zaixing,et al.Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro[J].J Third Mil Med Univ,2012,34(24):2498-2502.
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淋巴细胞微粒刺激AKT/Foxo1阻滞气道上皮细胞周期的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第24期
页码:
2498-2502
栏目:
论著
出版日期:
2012-12-30

文章信息/Info

Title:
Lymphocyte particles stimulates AKT/Foxo1 blocking airway epithelial cell cycle in vitro
作者:
焦燕邱倩杨再兴但小苹熊玮
第三军医大学西南医院呼吸内科
Author(s):
JiaoYan Qiu Qian Yang Zaixing Dan Xiaoping Xiong Wei
Department of Respiratory Diseases, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
细胞周期阻滞淋巴细胞微粒Foxo1AKTP21P27p-Foxo1p-AKT
Keywords:
cell cycle arrest lymphocyte microparticles Foxo1 AKT P21 P27 p-Foxo1 p-AKT
分类号:
R392.11;R392.32;R560.2
文献标志码:
A
摘要:
目的      研究淋巴细胞微粒(lymphocyte microparticles,LMPs)作用于气道上皮细胞引起周期阻滞在G1期的机制通路。      方法      将人类正常气道上皮细胞培养到对数生长期时,20 μg/ml的LMPs按照不同的时间0、4、8、16、20、24 h刺激气道上皮细胞,用RT-PCR方法检测P21、P27在mRNA水平的表达情况。20 μg/ml的LMPs按照不同的时间0、4、8、16、24 h刺激气道上皮细胞,用Western blot 方法检测P21、P27、Foxo1、p-Foxo1、AKT、p-AKT的蛋白表达情况。用免疫细胞化学技术检测Foxo1的核定位情况。      结果      与0 h对比,20 μg/ml的LMPs作用于气道上皮细胞能使P21、P27的mRNA、蛋白水平显著升高(P<0.01),Foxo1蛋白水平无明显变化(P>0.05),p-Foxo1的蛋白水平表达减弱(P<0.01),AKT蛋白水平无明显变化(P>0.05),p-AKT蛋白水平表达减弱(P<0.01)。      结论      LMPs作用于气道上皮细胞上调P21、P27蛋白的表达引起周期阻滞G1期,可能是通过AKT/Foxo1信号通路调节。
Abstract:
Objective      To determine the effect of lymphocyte microparticles (LMPs) on the cell cycle of airway epithelial cells and investigate the underlying mechanism.       Methods      LMPs of 20 μg/ml was treated human normal airway epithelial cells at logarithmic growth period for 0, 4, 8, 16, 20 and 24 h respectively. RT-polymerase chain reaction (PCR) was used to detect the expression of P21 and P27 at mRNA level. Western blot analysis was employed to test the expression of P21, P27, Foxo1, p-Foxo1, AKT and p-AKT at protein level. Immunocytochemical assay was to test the nuclear localization of Foxo1.       Results      LMPs of 20 μg/ml resulted in an obvious increase of P21 and P27 at mRNA and protein levels (P<0.01). No change was found in Foxo1 and AKT protein levels (P>0.05), but their phosphorylated proteins were evidently down-regulated (P<0.01).       Conclusion      LMPs upregulates P21 and P27 expression and causes cell cycle arrested at G1 phase in the airway epithelial cells through AKT/Foxo1 signaling pathways.

参考文献/References:

焦燕, 邱倩, 杨再兴, 等. 淋巴细胞微粒刺激AKT/Foxo1阻滞气道上皮细胞周期的研究[J]. 第三军医大学学报,2012,34(24):2498-2502.

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更新日期/Last Update: 2012-12-19