[1]向莉,李鹏,张竹君,等.IBP表达抑制后活化T淋巴细胞基因表达谱变化分析[J].第三军医大学学报,2013,35(02):109-113.
 Xiang Li,Li Peng,Zhang Zhujun,et al.Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray[J].J Third Mil Med Univ,2013,35(02):109-113.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第02期
页码:
109-113
栏目:
论著
出版日期:
2013-01-30

文章信息/Info

Title:
Gene expression profiles of activated T lymphocyte with IBP deficient by oligonucleotide microarray
作者:
向莉李鹏张竹君杨明珍陈安胡川闽
第三军医大学医学检验系临床生物化学教研室
Author(s):
Xiang Li Li Peng Zhang Zhujun Yang Mingzhen Chen An Hu Chuanmin
Department of Clinical Biochemistry, College of Laboratory Medicine, Third Military Medical University, Chongqing, 400038, China
关键词:
干扰素调节因子-4结合蛋白T淋巴细胞基因芯片
Keywords:
IRF-4 binding proteinT lymphocytegene chip
分类号:
R392.12;R394-33;R394.3
文献标志码:
A
摘要:
目的      利用全基因组寡核苷酸芯片检测干扰素调节因子-4结合蛋白(IRF-4 binding protein,IBP)表达抑制后活化T淋巴细胞基因表达谱的差异。      方法      采用本室构建的IBP表达抑制的Jurkat T细胞及相应对照细胞为实验对象,Anti-CD3、CD28 mAb处理细胞后提取刺激24、48 h的细胞总RNA,利用北京博奥生物有限公司22K Human Genome Array芯片检测基因表达的差异,以MAS.doc.V1软件,结合KEGG、NCBI等生物信息学数据库检索分析差异表达基因功能和网络关系。      结果      IBP基因表达抑制的Jurkat T细胞在TCR信号刺激24、48 h后,细胞能量代谢、周期生长、转录调控及凋亡等多种类型的基因发生差异表达改变,2个时相组共有56个差异表达趋势一致基因,其中17个基因共同上调,39个基因共同下调。      结论      在活化的Jurkat T细胞中IBP表达抑制所致多个基因差异表达。
Abstract:
Objective      To investigate the difference of gene expression between activated IBP-deficient and wild T lymphocyte by oligonucleotide microarray in order to determine the role of IBP in T lymphocyte activation.       Methods      Total RNA was isolated from IBP-deficient and its parental Jurkat T cells with anti-CD3 and anti-CD28 mAb stimulation for 24 or 48 h, and synthesized into double-stranded cDNA that was then synthesized into biotin-labeled cRNA probe by in vitro transcription. The cRNA probes were separately hybridized with 22K Human Genome Array Chip, and the signals were scanned by the GeneArray Scanner. The results were analyzed by bioinformatics.       Results      In comparison with the expression profile of parental Jurkat T cells, anti-CD3 and anti-CD28 mAb stimulation for 24 or 48 h resulted in that 56 genes were found to have no change in the expression, 17 genes were up-regulated and 39 genes down-regulated in activated IBP-deficient Jurkat T cells. According to Gene Ontology and Tree View analysis, these genes were involved in energy metabolism, cell cycle, transcription and apoptosis and so on.       Conclusion      IBP-deficient causes differential expression in many genes in Jurkat T cells.

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更新日期/Last Update: 2013-01-18