[1]高诗豪,唐政恒,陈图南,等.蜕皮甾酮对大鼠脑组织自由基损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(23):2357-2359.
　Gao Shihao,Tang Zhengheng,Chen Tunan,et al.Ecdysterone protects brain injury against free radical in rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(23):2357-2359.
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第23期
页码:
2357-2359
栏目:
论著
出版日期:
2012-12-15

文章信息/Info

Title:
Ecdysterone protects brain injury against free radical in rats
作者:
高诗豪; 唐政恒; 陈图南; 李飞; 单佑安; 冯华
第三军医大学西南医院神经外科，全军神经外科研究所，全军神经创伤防治重点实验室
Author(s):
Gao Shihao;  Tang Zhengheng;  Chen Tunan;  Li Fei;  Shan You’an;  Feng Hua
Department of Neurosurgery, Institute of Neurosurgery, Key Laboratory of Neurotrauma Prevention and Treatment, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
蜕皮甾酮; 脑损伤; 死亡率; 脑水肿; 自由基
Keywords:
ecdysterone;  traumatic brain injury;  death rate;  brain edema;  free radical
分类号:
R362; R651.15; R977
文献标志码:
A
摘要:
目的      观察蜕皮甾酮（ecdysterone, EDS）对大鼠脑损伤的治疗作用。      方法        SD雄性大鼠120只，按随机数字表法分6组，每组20只。采用Feeney法自由落体打击鼠脑造模。假手术组动物只开颅，不打击；治疗组：分别采用4、8、16 mg/kg的EDS腹腔注射模型动物；阴性治疗对照组：模型动物腹腔注射生理盐水；阳性治疗对照组：模型动物腹腔注射3 mg/kg依达拉奉。伤后7 d计算各组动物死亡率；检测各组动物脑组织含水量及丙二醛（MDA）、羟自由基能力及超(过)氧化物歧化酶（superoxide dismutase，SOD）等。HE染色观测各组大鼠脑组织海马病变情况。      结果        伤后7 d，16 mg/kg EDS治疗组的死亡率、脑组织含水量MDA与羟自由基含量较阴性治疗对照组明显降低(P<0.05)，SOD活性显著升高(P<0.01)，脑细胞损害明显减轻，大鼠海马CA1区正常神经元数量明显优于阴性治疗组（P<0.01）。      结论        蜕皮甾酮对脑损伤具有保护治疗作用。
Abstract:
Objective        To investigate the effect of ecdysterone (EDS) on traumatic brain injury (TBI) in rats.       Methods       A total of 120 male Sprague-Dawley rats were randomly and equally divided into 6 groups, sham-operation group, TBI group, edaravone treatment group and EDS treatment groups. Modified Feeney’s method was used to establish the rat model of experimental TBI with an impact of a weight of 30 g from 20 cm after craniotomy. Rats of EDS treatment groups were given EDS at 4, 8, and 16 mg/kg via peritoneal injection for 7 d. The other 3 groups were treated with normal saline or edaravone (3 mg/kg). The animal death rate was calculated, and the changes of the brain water content, malondialdehyde (MDA), and superoxide dismutase（SOD）in brain tissue were calculated and measured. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of brain hippocampus tissue.       Results        EDS (16 mg/kg) resulted in significant decreases in the death rate, brain water content, and MDA content (P<0.05), and an obvious increase in SOD content (P<0.01) compared with TBI group. EDS (16 mg/kg) also resulted in lesser damage in the brain cells, with the pyramidal cells number significantly higher than TBI  group (P<0.01).       Conclusion        EDS exerts therapeutic effect on TBI through inhibiting free radical damage and brain edema.

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更新日期/Last Update: 2012-12-04