[1]宗兆文,陈思旭,贾敏,等.Osterix在调控脊柱发育中的作用[J].第三军医大学学报,2013,35(03):220-222.
 Zong Zhaowen,Chen Sixu,Jia Min,et al.Role of Osterix in development of spine in mice[J].J Third Mil Med Univ,2013,35(03):220-222.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第03期
页码:
220-222
栏目:
论著
出版日期:
2013-02-15

文章信息/Info

Title:
Role of Osterix in development of spine in mice
作者:
宗兆文陈思旭贾敏沈岳赵玉峰郭庆山华翔Jerry Feng
第三军医大学大坪医院野战外科研究所创伤科,创伤、烧伤与复合伤国家重点实验室;德克萨斯州卫生科学中心Baylor医学院
Author(s):
Zong Zhaowen Chen Sixu Jia Min Shen Yue Zhao Yufeng Guo Qingshan Hua Xiang Jerry Feng
State Key Laboratory of Trauma, Burs and Combined Injury, Department of Trauma, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China; Medical College of Baylor, Health and Science Center of Texas, USA
关键词:
Osterix脊柱发育基因敲除
Keywords:
Osterix spine development knockout
分类号:
R336;R394.1;R682.3
文献标志码:
A
摘要:
目的      观察核转录因子Osterix在脊柱发育中的作用。      方法        采用Lox P/Cre系统,以2.3 kb Ⅰ型胶原蛋白为启动子,在小鼠成骨细胞和骨细胞中特异性敲除Osterix,观察Osterix敲除小鼠大体情况,采用X线摄片、HE染色和Nissl染色观察其对小鼠脊柱发育的影响。      结果        Osterix敲除小鼠后肢瘫痪,行走时前肢运动正常,拖动后肢爬行,Osterix 的敲除效率约为75%;X线片结果显示,Osterix敲除小鼠形体变小,胸腰段脊柱出现楔形椎体、严重的脊柱侧弯和椎管狭窄等畸形;HE染色结果显示胸腰段脊椎结构发育严重异常;Nissl染色结果显示,受累节段脊髓神经元结构严重受损。      结论        Osterix在脊柱发育中起到重要作用,敲除后可导致脊柱侧弯等畸形。
Abstract:
Objective      To investigate the role of Osterix in spine development.       Methods      Osterix was knocked out by crossing 2.3 kb Collagen I Cre mice with Osterix Lox P +/+ mice. X-ray radiology, HE staining and Nissl staining were employed to observe the effect of Osterix knockout on spine development.       Results      Osterix knockout mice demonstrated short stature, wedged vertebrae, severe scoliosis and spinal stenosis at the thoracic and lumbar regions of spine. HE staining showed that the structure of growth plate, vertebral body and spinal canal were totally disorganized. Nissl staining revealed that the neurons in the spinal cord at the inflicted region were strikingly damaged.       Conclusion      Osterix plays an important role in spine development, and Osterix knockout leads to severe spinal deformities such as scoliosis.

参考文献/References:

宗兆文, 陈思旭, 贾敏, 等. Osterix在调控脊柱发育中的作用[J].第三军医大学学报,2013,35(3):220-222.

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更新日期/Last Update: 2013-01-29