[1]黄宇星,胡胜利,邹永杰,等.高压氧预适应对大鼠大脑中动脉闭塞后骨桥蛋白表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(01):15-19.
 Huang Yuxing,Hu Shengli,Zou Yongjie,et al.Effect of hyperbaric oxygen preconditioning on osteopontin expression in MCAO rats[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(01):15-19.
点击复制

高压氧预适应对大鼠大脑中动脉闭塞后骨桥蛋白表达的影响(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
35卷
期数:
2013年第01期
页码:
15-19
栏目:
论著
出版日期:
2013-01-15

文章信息/Info

Title:
Effect of hyperbaric oxygen preconditioning on osteopontin expression in MCAO rats
作者:
黄宇星胡胜利邹永杰陈图南李强李飞冯华
第三军医大学西南医院神经外科,全军神经外科研究所,全军神经创伤防治重点实验室
Author(s):
Huang Yuxing Hu Shengli Zou Yongjie Chen Tunan Li Qiang Li Fei Feng Hua
Department of Neurosurgery, Institute of Neurosurgery, Key Laboratory of Neurotrauma Prevention and Treatment, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
高压氧预适应大脑中动脉闭塞神经保护骨桥蛋白
Keywords:
hyperbaric oxygen preconditioning middle cerebral artery occlusion neuroprotection osteopontin
分类号:
R362; R459.6; R743.31
文献标志码:
A
摘要:
目的      观察高压氧预适应( hyperbaric oxygen preconditioning, HBOP)对大鼠大脑中动脉闭塞(middle cerebral artery occlusion, MCAO)后的神经保护作用并初步探讨其对骨桥蛋白(osteopontin, OPN)表达的影响。      方法        雄性SD大鼠72只,分为假手术组,HBOP+假手术组,单纯MCAO组、HBOP+MCAO组,每组18只。采用大脑中动脉线栓法建立大鼠MCAO模型。于术后12 h取材,观察大鼠神经功能和脑梗死变化情况;HE染色光镜下观察缺血区脑组织的病理形态学改变。Western blot检测缺血区OPN的表达变化。      结果       神经功能缺损评分:术后12 h假手术组及HBOP+假手术组评分均为0分,单纯MCAO组(3.80±0.79)分,HBOP+MCAO组(2.50±0.97)分;平衡能力评分:术后12 h假手术组及HBOP+假手术组大鼠平衡能力评分均为5分,单纯MCAO组(1.50±1.08)分,HBOP+MCAO组(2.70±2.95)分;脑梗死率:术后12 h假手术组及HBOP+假手术组大鼠梗死率均为0,单纯MCAO组(49.22±7.07)%,HBOP+MCAO组(41.96±3.15)%。可见与单纯MCAO组比较, HBOP+MCAO组在术后12 h神经功能缺损评分明显降低(P<0.05),平衡能力评分显著提高(P<0.05);脑梗死率明显小于单纯MCAO组(P<0.05) ;HE染色见缺血区脑组织病理损伤明显减轻;Western blot检测提示OPN的表达显著增强。      结论        高压氧预适应可以诱导大鼠MCAO后的神经保护作用,其机制可能与HBOP引起的OPN表达增高有关。
Abstract:
Objective         To observe the neuroprotective effect of hyperbaric oxygen preconditioning (HBOP) in rats after middle cerebral artery occlusion (MCAO), and to investigate the effect on osteopontin (OPN) expression.       Methods        Seventy-two Sprague-Dawley rats were randomly and equally divided into 4 groups including a sham group, a HBOP+sham group, a simple MCAO group and a HBOP+MCAO group (n=18). The neurological deficits and cerebral infarction volume were detected in the rats at 12 h after permanent MCAO. The pathological changes of ischemic regions were observed under a light microscope by HE staining, and the expression of OPN in ischemic regions was determined by Western blotting.       Results       The scores of neurological deficits at 12 h after MCAO were 0 in the sham and HBOP+sham groups, 3.80±0.79 in the simple MCAO group, and 2.50±0.97 in the HBOP+MCAO group. The scores of balance ability at 12 hours after MCAO were 5 in the sham and HBOP+sham groups, 1.50±1.08 in the simple MCAO group, and 2.70±2.95 in the HBOP+MCAO group. The proportions of cerebral infarction volume at 12 hours after MCAO were 0 in the sham and HBOP+sham groups, 49.22±7.07 in the simple MCAO group, and 41.96±3.15 in the HBOP+MCAO group. Compared with the simple MCAO group, the score of neurological deficits reduced significantly (P<0.05), the score of balance ability increased significantly (P<0.05), and the proportion of cerebral infarction volume was significantly lower in the HBOP+MCAO group at 12 h after MCAO (P<0.05). The pathological injury of brain tissues in ischemic region was significantly relieved, and the expression of OPN was upregulated in the HBOP+MCAO group.       Conclusion       HBOP plays a neuroprotective role in rats after MCAO, and the mechanism may be associated with the upregulation of OPN.

相似文献/References:

[1]刘海朋,胡永波,张小东,等.电凝法制作大鼠脑卒中模型的改良研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(17):1798.
 Liu Haipeng,Hu Yongbo,Zhang Xiaodong,et al.Improved electrocoagulation method for establishing rat cerebral apoplexy model[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(01):1798.
[2]李雨泓,邹永杰,崔高宇.高压氧预适应对离体培养大鼠脑星形胶质细胞的缺氧保护作用及机制[J].陆军军医大学学报(原第三军医大学学报),2014,36(09):893.
 Li Yuhong,Zou Yongjie,Cui Gaoyu.Hyperbaric oxygen preconditioning protects rat brain astrocytes from hypoxia by down-regulating AQP4 and R2X7 receptor in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(01):893.
[3]朱德辉.外伤性颈内动脉与大脑中动脉闭塞——附3例临床报告[J].陆军军医大学学报(原第三军医大学学报),1981,03(03):0.[doi:10.16016/j.1000-5404.1981.03.018 ]
[4]邹永杰,黄宇星,李雨泓,等.高压氧预适应的小鼠缺血脑保护作用及对P2X7受体表达的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(10):977.
 Zou Yongjie,Huang Yuxing,Li Yuhong,et al.Effects of hyperbaric oxygen preconditioning on expression of P2X7 receptor and its neuroprotection for cerebral ischemia in mice[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(01):977.

更新日期/Last Update: 2012-12-31