[1]程晓荣,杨爱军,刘燕菲,等.前胃泌素、胃泌素、膜联蛋白2对胃癌CD44+细胞增殖和侵袭的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2171-2175.
 Cheng Xiaorong,Yang Aijun,Liu Yanfei,et al.Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44+ gastric cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(21):2171-2175.
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前胃泌素、胃泌素、膜联蛋白2对胃癌CD44+细胞增殖和侵袭的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第21期
页码:
2171-2175
栏目:
论著
出版日期:
2012-11-15

文章信息/Info

Title:
Effects of progastrin, gastrin and annexin 2 on proliferation and invasion in CD44+ gastric cancer cells
作者:
程晓荣杨爱军刘燕菲李双明向琳杨丽李敏
兰州大学基础医学院病理学研究所;甘肃省新药临床前研究重点实验室;兰州军区总医院病理科
Author(s):
Cheng Xiaorong Yang Aijun Liu Yanfei Li Shuangming Xiang Lin Yang Li Li Min
Institute of Pathology, College of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu Province, 730000;Key Lab of Preclinical Study for New Drugs of Gansu Province, Lanzhou, Gansu Province, 730000; Department of Pathology, General Hospital of Lanzhou Military Command, Lanzhou, Gansu Province, 730050, China
关键词:
胃癌BGC-823细胞CD44前胃泌素胃泌素膜联蛋白2
Keywords:
gastric cancer cell line BGC-823CD44progastringastrin annexin 2
分类号:
R73-37;R730.23;R735.2
文献标志码:
A
摘要:
目的      检测前胃泌素、胃泌素、膜联蛋白2在CD44+细胞的表达情况,研究三者对CD44+细胞生长和侵袭能力的影响。      方法       使用CD44免疫磁珠分选技术从胃癌BGC-823细胞中分选出CD44+细胞,流式细胞仪(FCM)和免疫荧光检测CD44+细胞中CD44的表达,以鉴定分选是否成功。FCM细胞周期分析和无血清培养,检测CD44+细胞的干细胞特性。流式细胞仪检测前胃泌素、胃泌素、膜联蛋白2在CD44+细胞、BGC-823细胞和CD44-细胞表达的差异。 MTT法和Boyden小室实验研究3者对CD44+细胞生长和侵袭能力的影响。      结果      流式细胞仪和细胞免疫荧光结果提示:CD44+细胞中CD44阳性率为(90.20±2.24)%,显著高于BGC-823细胞[(9.57±2.35)%,P<0.01];CD44+细胞组处于G0/G1期的细胞为(71.1±5.6)%,显著高于BGC-823细胞组[(47.7±3.5)%,P<0.05];在无血清培养7 d后,CD44+细胞能够形成圆形克隆球,而CD44-细胞不能形成圆形克隆球;流式细胞仪显示前胃泌素、胃泌素、膜联蛋白2在CD44+细胞中高表达,明显高于在BGC-823细胞和CD44-细胞的表达水平(P<0.01);Pearson相关性分析显示:三者的表达率呈正相关关系(P<0.01)。高浓度的前胃泌素mAb和膜联蛋白2mAb能够抑制CD44+细胞的生长和侵袭,与对照组相比差异显著(P<0.05)。胃泌素mAb不能抑制CD44+细胞的生长和侵袭,与对照组相比差异不显著(P>0.05)。      结论      CD44+细胞中高表达前胃泌素、胃泌素、膜联蛋白2,并且前胃泌素mAb和膜联蛋白2mAb能够抑制CD44+细胞的生长和侵袭能力,提示前胃泌素与膜联蛋白2能够促进CD44+细胞的生长和侵袭。
Abstract:
Objective      To investigate the expression of progastrin, annexin 2, and gastrin in CD44+gastric cancer cells, and determine their effects on the proliferation and invasion in the CD44+ cells.       Methods      CD44 immunomagnetic beads were used to sort CD44+cells from gastric cell line BGC-823, and the sorted cells were identified by flow cytometry and cell immunofluorescence for the expression of CD44. Flow cytometry-cycle analysis and serum-free culture methods were used to examine CD44+ cells’ characteristics of stem cells. The expression of progastrin, gastrin, and annexin 2 in CD44+ cells, BGC-823 cells and CD44- cells were detected by flow cytometry, cell immunofluorescence and immunohistochemistry assays. The effect of them on the proliferation and invasion in the CD44+cells were detected by MTT assay and Boyden chamber.       Results      Flow cytometry and cell immunofluorescence assay showed the expression of CD44 in CD44+cells [(90.20±2.24)%] was markedly higher than in BGC-823 cells [(9.57±2.35)%, P<0.01]. There were (71.1±5.6)% CD44+cells arrested in G0/G1 phase, which was obviously higher than that in BGC-823 cells [(47.7±3.5)%, P<0.05]. The CD44+ cells formed circular clone balls under serum free culture for 7 d, while the CD44- cells could not. The expression of progastrin, gastrin and annexin 2 in CD44+ cells was significantly higher than in BGC-823 cells and CD44- cells (P<0.01). Pearson correlation analysis showed that there was a positive correlation among their expression (P<0.01). High concentrations of progastrin mAb and annexin2 mAb inhibited the proliferation and invasion of CD44 cells, and there was statistical significance compared with control group (P<0.05), but gastrin mAb could not (P>0.05).       Conclusion      CD44+ cells widely express progastrin, gastrin, and annexin 2. Progastrin mAb and annexin2 mAb inhibit  the proliferation and invasion, which suggest that these two may promote cell proliferation and invasion.

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更新日期/Last Update: 2012-11-06