[1]杨勇,王晓娟,刘德贵,等.IL-18增加烧伤休克复苏大鼠中性粒细胞中超氧阴离子的生成[J].第三军医大学学报,2012,34(19):1965-1967.
 Yang Yong,Wang Xiaojuan,Liu Degui,et al.IL-18 increases superoxide anion production in neutrophils of rats during resuscitation following burn shock[J].J Third Mil Med Univ,2012,34(19):1965-1967.
点击复制

IL-18增加烧伤休克复苏大鼠中性粒细胞中超氧阴离子的生成(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第19期
页码:
1965-1967
栏目:
论著
出版日期:
2012-10-15

文章信息/Info

Title:
IL-18 increases superoxide anion production in neutrophils of rats during resuscitation following burn shock
作者:
杨勇王晓娟刘德贵薛刚
成都军区总医院烧伤整形科
Author(s):
Yang Yong Wang Xiaojuan Liu Degui  Xue Gang
Department of Burn and Plastic Surgery, General Hospital of Chengdu Military Command, Chengdu, Sichuan Province, 610083, China
关键词:
白细胞介素18烧伤休克延迟复苏 超氧化物类羟自由基
Keywords:
interleukin-18 burn shock delayed fluid resuscitation superoxide anion hydroxy free radical
分类号:
R392.11; R392.12; R644.02
文献标志码:
A
摘要:
目的      探讨在烧伤休克复苏大鼠模型中,IL-18与中性粒细胞释放氧自由基——超氧阴离子和羟自由基的关系以及相应的作用。      方法      建立烧伤休克延迟复苏模型大鼠,分别检测假烧伤组以及延迟复苏大鼠在模型建立后6、12、24、48 h体内白介素-18(interleukin 18 ,IL-18)以及中性粒细胞的含量。腹腔注射IL-18(20 μg/kg)以及抗大鼠IL-18抗体(anti-rat-IL-18 antibody,80 μg/kg)后,检测模型大鼠中性粒细胞内超氧自由基以及羟自由基的含量。将新鲜分离的中性粒细胞体外培养,分别加入IL-18(0.5 μg/ml)以及anti-rat-IL-18 抗体(0.5 μg/ml),共培养24 h后,检测中性粒细胞内超氧自由基以及羟自由基的含量。      结果       大鼠烫伤后6、12、24、48 h外周血IL-18的含量[(51.46±11.36)、(64.86±12.05)、(78.64±15.64)、(69.44±12.28) pg/ml]以及中性粒细胞的数量[(38.46±13.57)×105、(128.45±45.78)×105、(245.25±84.15)×105、(179.56±49.27)×105/ml]均显著提高,且变化趋势相同(P<0.01)。腹腔注射IL-18大鼠中性粒细胞内超氧阴离子以及8-羟基脱氧鸟苷(8-OHdG)的含量均显著高于假烧伤组[(0.45±0.03) vs (0.15±0.02), (605±145) vs  (152±21) pg/ml,P<0.01],而注射anti-rat-IL-18抗体的大鼠中性粒细胞内超氧阴离子以及8-OHdG的含量均显著低于烧伤大鼠延迟复苏组[(0.19±0.03)  vs  (0.32±0.04),(198±45)  vs  (524±124) pg/ml,P<0.01]。离体条件下,加入IL-18的中性粒细胞在体外超氧阴离子以及8-OHdG的含量均显著高于对照组[(0.38±0.02) vs  (0.13±0.02), (505±99) vs  (92±21) pg/ml,P<0.01],而注射anti-rat-IL-18抗体的大鼠中性粒细胞内超氧阴离子以及8-OHdG的含量均显著低于烧伤组大鼠[(0.16±0.03)  vs  (0.29±0.03), (95±45)  vs  (324±68)pg/ml,P<0.01]。      结论      在烧伤休克延迟复苏大鼠中,IL-18能够有效地与大鼠体内中性粒细胞结合,促进烧伤休克延迟复苏大鼠模型中的氧自由基的生成,进一步造成组织的损伤。
Abstract:
Objective      To study the role of IL-18 and its relation with superoxide anion production and hydroxyl free radical released from neutrophils in rat resuscitation model following burn shock.       Methods      At 6, 12, 24 and 48 h after a rat resuscitation model following burn shock was established, the peripheral IL-18 level was measured and the number of neutrophils in the blood sample was calculated. The number of superoxide free radicals and hydroxy free radicals in neutrophils of the rat model was calculated after intraperitoneal injection with IL-18 (20 g/kg) and anti-rat-IL-18 antibody (80 μg/kg). The freshly isolated neutrophils were cultured in vitro, into which IL-18 (0.5 μg/ml) and anti-rat-IL-18 antibody (0.5 μg/ml) were added. The number of superoxide free radicals and hydroxy free radicals in neutrophils was calculated in 24 h after culture.       Results      The level of peripheral IL-18 (51.46±11.36, 64.86±12.05, 78.64±15.64 and 69.44±12.28 pg/ml) and the number of peripheral neutrophils [(38.46±13.57)×105/ml, (128.45±45.78)×105/ml, (245.25±84.15)×105/ml and (179.56±49.27)×105/ml] were significantly higher in resuscitation group than in control group at 6, 12, 24 and 48 h after burn (P<0.01). The superoxide anion and 8-OHdG levels were significantly higher in rats injected with IL-18 than in those with sham burn (0.45±0.03 vs 0.15±0.02, 605±145 vs 152±21, 605±145 vs 152±21 pg/ml, P<0.01) and significantly lower in rats injected with anti-rat-IL-18 antibody than in those after delayed resuscitation (0.19±0.03 vs 0.32±0.04, 198±45 vs 524±124 pg/ml, P<0.01). The superoxide anion and 8-OHdG levels were significantly higher in neutrophils into which IL-18 was added than in those into which IL-18 was not added (0.38±0.02 vs 0.13±0.02, 505±99  vs 92±21 pg/ml, P<0.01) and significantly lower in rats injected with anti-rat-IL-18 antibody than in those with burn (0.16±0.03 vs 0.29±0.04, 95±45 vs 324±68 pg/ml, P<0.01).       Conclusion      IL-18 can effectively bind to neutrophils in rats during delayed resuscitation and promote production of superoxide free radicals in rat resuscitation model following burn shock, thus leading to further tissue damage.

参考文献/References:

杨勇, 王晓娟, 刘德贵, 等. IL-18增加烧伤休克复苏大鼠中性粒细胞中超氧阴离子的生成[J]. 第三军医大学学报,2012,34(19):1965-1967.

相似文献/References:

[1]李淑蓉,粟永萍,邓正阳,等.烧伤小鼠早期P56与IFN mRNA表达变化及麻醉或KPV对其表达的影响[J].第三军医大学学报,2008,30(05):367.
 LI Shu-rong,SU Yong-ping,DENG Zheng-yang,et al.Expressions of P56, IFN-α and IFN-β mRNAs in major organs of severe burn mice and effects of KPV and anaesthesia[J].J Third Mil Med Univ,2008,30(19):367.
[2]王广,黄跃生,罗中华,等.模拟海上颠簸刺激对严重烧伤家兔心肌力学指标的影响[J].第三军医大学学报,2008,30(08):702.
 WANG Guang,HUANG Yue-sheng,LUO Zhong-hua,et al.Effects of simulated toss on myocardial mechanics following early burn injury in rabbits[J].J Third Mil Med Univ,2008,30(19):702.
[3]何梅,覃霞,陈军,等.烧伤患者抑郁情绪障碍发病特点的研究[J].第三军医大学学报,2008,30(14):1371.
 HE Mei,QIN Xia,CHEN Jun,et al.Characteristics of depression occurred in burned patients[J].J Third Mil Med Univ,2008,30(19):1371.
[4]郭晓姝,方海立,李军,等.颈交感神经阻滞对烧伤早期小鼠肝脏干扰素诱导蛋白IFIT1表达的影响[J].第三军医大学学报,2007,29(17):1647.
 GUO Xiao-shu,FANG Hai-li,LI Jun,et al.Effect of cervical sympathetic block on hepatic interferon-induced protein IFIT1 in burned mice during early stage[J].J Third Mil Med Univ,2007,29(19):1647.
[5]陈传莉,刘依凌,王裴,等.严重烧伤后肠黏膜肌球蛋白轻链磷酸化表达改变及其意义[J].第三军医大学学报,2008,30(15):1434.
 CHEN Chuan-li,LI Yi-ling,WANG Pei,et al.Role of MLC phosphorylation in intestinal epithelial barrier dysfunction induced by severe burn injury[J].J Third Mil Med Univ,2008,30(19):1434.
[6]李军,粟永萍,王军平,等.颈交感神经阻滞对小鼠烧伤早期肝组织核因子-κB及其辅活化子的影响[J].第三军医大学学报,2007,29(08):655.
 LI Jun,SU Yong-ping,WANG Jun-ping,et al.Effect of cervical sympathetic block on hepatic NF-κB and its coactivator during the early stage in burned mice[J].J Third Mil Med Univ,2007,29(19):655.
[7]王海燕,张黎明,朱明学,等.氧自由基在大鼠硫芥中毒复合烧伤小肠损伤中的影响[J].第三军医大学学报,2006,28(22):2223.
[8]王锋超,王涛,艾国平,等.不同伤情血清可有效激活IEC-6细胞PI3K/Akt通路[J].第三军医大学学报,2006,28(06):518.
[9]曾东,余文林,刘宏伟,等.超脉冲CO2激光切痂的临床应用[J].第三军医大学学报,2006,28(20):2087.
[10]王艺明,杨宗城.去甲肾上腺素对严重烧伤大鼠脑水肿的影响[J].第三军医大学学报,2005,27(18):1833.

更新日期/Last Update: 2012-09-27