[1]颜婷,孙萍,陈婷婷,等.IL-4、IFN-γ及LTE4浓度在婴幼儿喘息性疾病中的变化及其意义[J].陆军军医大学学报(原第三军医大学学报),2012,34(18):1873-1876.
　Yan Ting,Sun Ping,Chen Tingting,et al.Change and significance of serum IL-4 and IFN-γ and urinary leukotriene E4 in infants with asthmatic diseases[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(18):1873-1876.
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IL-4、IFN-γ及LTE4浓度在婴幼儿喘息性疾病中的变化及其意义(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第18期
页码:
1873-1876
栏目:
论著
出版日期:
2012-09-30

文章信息/Info

Title:
Change and significance of serum IL-4 and IFN-γ and urinary leukotriene E4 in infants with asthmatic diseases
作者:
颜婷; 孙萍; 陈婷婷; 王倩; 黄正刚
南昌大学:研究生院，第一附属医院儿科
Author(s):
Yan Ting;  Sun Ping;  Chen Tingting; Wang Qian;  Huang Zhenggang
School of Postgraduate, Department of Pediatrics, First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi Province, 330006, China
关键词:
喘息; 白细胞介素-4; 干扰素-γ; 白三烯E4; 婴幼儿
Keywords:
asthma;  interleukin-4 ; interferon-γ;  leukotriene E4;  infants
分类号:
R392.11;R392.3;R725.6
文献标志码:
A
摘要:
目的      探讨患喘息性疾病的婴幼儿血清白介素-4（IL-4）、干扰素-γ（IFN-γ）及尿白三烯E4（LTE4）浓度的变化及其意义。      方法      采用竞争性酶联免疫吸附法（ELISA）检测22例急性期、恢复期喘息性疾病患儿（喘息组），20例急性期非喘息的下呼吸道感染患儿（非喘息组），20例健康体检儿（正常对照组）血清IL-4、IFN-γ及尿LTE4水平，分析IL-4与IFN-γ的比值，分析IL-4、IFN-γ与尿LTE4的相关性。      结果      喘息组急性期血清IL-4水平较非喘息组急性期及正常对照组均明显升高（P<0.05，0.01）；血清IFN-γ水平明显降低(P<0.01)；尿LTE4水平较正常对照组明显升高(P<0.01)，但与非喘息组急性期比较差异无统计学意义（P>0.05）；喘息组恢复期血清IL-4水平较急性期明显下降(P<0.01)，但仍高于正常对照组(P<0.01)；血清IFN-γ水平明显升高(P<0.01)，但仍低于正常对照组(P<0.01)；尿LTE4水平明显降低(P<0.01)，但仍高于正常对照组(P<0.01)。喘息组急性期血清IL-4/IFN-γ比值较非喘息组急性期及正常对照组均明显升高（F=15.86，P<0.01）；恢复期血清IL-4/IFN-γ比值较急性期明显下降(t=4.14，P<0.01)，但仍高于正常对照组，且有统计学差异(t=5.94，P<0.01)。喘息组急性期尿LTE4水平与血清IL-4水平呈正相关（r=0.82，P<0.01），与血清IFN-γ水平呈负相关（r=-0.81，P<0.01）。      结论      IL-4、IFN-γ及LTE4可能共同参与婴幼儿喘息性疾病的气道炎症反应，喘息患儿存在Th1/Th2免疫失衡。
Abstract:
Objective      To investigate the changes in serum levels of interleukin-4 (IL-4), interferon-γ（IFN-γ）and urinary level of leukotriene E4(LTE4) in infants with asthmatic diseases and their significances.       Methods      Serum levels of IL-4 and IFN-γ and urinary levels of LTE4 were measured by competitive ELISA in 22 infants with acute and convalescence asthmatic diseases (asthmatic group), 20 infants with acute non-asthmatic diseases but with respiratory tract infection (non-asthmatic group), and 20 healthy infants (normal control group). The ratio of IL-4 to IFN-γ and the correlations between urinary LTE4 and serum IL-4, and between urinary LTE4 and serum IFN-γ were analyzed.       Results      The serum levels of IL-4 in acute asthma period was obviously higher than that of acute non-asthma and normal control（P<0.05, 0.01）. The serum levels of IFN-γ in acute asthma period was significantly lower than those of acute non-asthma and normal control（P<0.01）. The urinary levels of LTE4 in acute asthma period was higher than normal control group（P<0.01）, but were not significantly different from acute non-asthma （P>0.05）. In remission/convalescence, the serum levels of IL-4 were lower than those of acute period (P<0.01), but were still higher than normal control group (P<0.01). The serum levels of IFN-γ were higher than those of acute period (P<0.01), but were still lower than normal control group (P<0.01). The urinary levels of LTE4 were obviously lower than those of acute period (P<0.01), but were still higher than normal control (P<0.01). The ratio of IL-4 to IFN-γ in acute asthma period was higher than that of acute non-asthma and normal control group（F=15.86，P<0.01）.In remission, the ratio of IL-4 to IFN-γ was lower than that in acute period (t=4.14, P<0.01), but was still higher than normal control group (P<0.01). There was positively correlation between urinary LTE4 and serum IL-4 levels in the acute stage in asthmatic group（r=0.82, P<0.01）. There was negative correlation between urinary LTE4 and serum IFN-γ levels in the acute stage in asthmatic group（r=-0.81, P<0.01).       Conclusion      IL-4, IFN-γ and LTE4 may all be involved in the pathogenesis of respiratory tract inflammation in infant with asthmatic diseases. There are immune imbalance of Th1/Th2 in infant with wheezing.

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更新日期/Last Update: 2012-09-18