[1]陈志雄,张军,张刘平,等.胃转流术降低2型糖尿病大鼠肝脏FoxO1因子的表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(17):1766-1770.
　Chen Zhixiong,Zhang Jun,Zhang Liuping,et al.Effect of gastric bypass on FoxO1 expression in liver of rats with type 2 diabetes[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(17):1766-1770.
点击复制

胃转流术降低2型糖尿病大鼠肝脏FoxO1因子的表达(/HTML )
分享到：

陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第17期
页码:
1766-1770
栏目:
论著
出版日期:
2012-09-15

文章信息/Info

Title:
Effect of gastric bypass on FoxO1 expression in liver of rats with type 2 diabetes
作者:
陈志雄; 张军; 张刘平; 寇耀; 蒙长远; 张能; 汪志红
重庆医科大学附属第一医院胃肠外科
Author(s):
Chen Zhixiong;  Zhang Jun;  Zhang Liuping;  Kou Yao;  Meng Changyuan;  Zhang Neng;  Wang Zhihong
Department of Gastrointestinal Surgery,  First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
胃转流术; 2型糖尿病; FoxO1
Keywords:
gastric bypass;  type 2diabetes mellitus;  FoxO1 (FKHR)
分类号:
R363.272; R587.1; R656.61
文献标志码:
A
摘要:
目的      研究胃转流术对2型糖尿病大鼠肝脏FoxO1表达的影响。      方法       40只SD大鼠随机分为糖尿病手术组（A组）、假手术组（B组）、正常对照组（C组）、糖尿病对照组（D组），每组10只。C、D组按A、B组术前和术后4、8、16、24周检测空腹血糖、空腹和餐后血清胰岛素、胰高血糖素样肽（GLP-1）。24周后处死各组大鼠，免疫组化和Western blot检测各组肝脏中FoxO1的表达。      结果       A组术后4周空腹血糖由术前的（20.2±2.1）mmol/L降至（10.5±2.5）mmol/L，术后24周空腹血糖为（7.7±1.1）mmol/L，术后4周空腹胰岛素由术前（2.87±0.08）mU/L增至（2.91±0.08）mU/L，术后24周空腹胰岛素为（3.00±0.06）mU/L；术后4周空腹血清GLP-1由术前（8.7±0.9）pmol/L升至（13.2±1.0）pmol/L，术后24周为（23.5±0.2）pmol/L。A组术后24周肝脏FoxO1表达量为18.4±1.2，B组为37.6±0.5，C、D两组分别为9.2±1.0、35.8±0.4，A组术后FoxO1的表达量明显低于B、D组，差异有统计学意义（P＜0.05）。A组血清GLP-1浓度与肝脏FoxO1的表达量成负相关（r=-0.864,P<0.05）。      结论       胃转流术可显著降低2型糖尿病大鼠肝脏FoxO1的表达，降低血糖。
Abstract:
Objective       To investigate the effect of gastric bypass on FoxO1 expression in the liver of rats with type 2 diabetes, and to explore the mechanism for gastric bypass ameliorating type 2 diabetes.       Methods       Forty SD rats were randomly divided into a diabetes operation group (group A, n=10), a diabetes sham operation group (group B, n=10), a normal control group (group C, n=10) and a diabetes group (group D, n=10). The levels of fasting blood glucose (FBG), fasting and postprandial insulin and glucagon-like peptide-1(GLP-1) were measured before operation and at 4, 8, 16 and 24 weeks after operation. All the rats were sacrificed after 6 months, and the expression of FoxO1 in rat livers was determined by immunohistochemistry and Western blotting.       Results       As for the group A, the level of FBG decreased from 20.2±2.1 mmol/L before operation to 10.5±2.5 mmol/L at 4 weeks and 7.7±1.1 mmol/L at 24 weeks after operation, respectively. The level of fasting insulin increased from 2.87±0.08 mU/L before operation to 2.91±0.08 mU/L at 4 weeks and 3.0±0.06 mU/L at 24 weeks after operation, respectively. The level of fasting GLP-1 increased from 8.7±0.9 pmol/L before operation to 13.2±1.0 pmol/L at 4 weeks and 23.5±0.2 pmol/L at 24 weeks after operation, respectively. The expression level of FoxO1 was significantly lower in the group A (18.4±1.2) than in the group B (37.6±0.5) and group D (35.8±0.4) (P<0.05), and the FoxO1 expression was positively correlated with the serum level of GLP-1 in the group A (r=-0.864, P<0.05).       Conclusion       Gastric bypass can significantly reduce the levels of blood glucose and FoxO1 expression in the liver of rats with type 2 diabetes.

相似文献/References:

[1]李硕,倪向敏,徐喆,等.雌马酚对2型糖尿病大鼠的肾脏保护作用[J].陆军军医大学学报(原第三军医大学学报),2022,44(10):1009.
　LI Shuo,NI Xiangmin,XU Zhe,et al.Renal protective effect of equol on type 2 diabetic rats [J].J Amry Med Univ (J Third Mil Med Univ),2022,44(17):1009.
[2]李亚,李伟,王晶,等.抑郁状态与2型糖尿病形成各阶段代谢指标的相关性分析[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1697.
[3]金晖,鲍洁,赵镇,等.米格列奈与瑞格列奈对新诊断2型糖尿病的临床疗效研究[J].陆军军医大学学报(原第三军医大学学报),2013,35(03):260.
　Jin Hui,Bao Jie,Zhao Zhen,et al.Clinical efficacy of mitiglinide and repaglinide in treatment of patients with newly diagnosed type 2 diabetes mellitus[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(17):260.
[4]廖涌,章婧,李阳,等.甘精胰岛素联合门冬胰岛素在2型糖尿病围手术期的临床应用[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1766.
　LIAO Yong,ZHANG Jing,LI Yang,et al.Insulin glargine plus insulin aspart for perioperative patients with type 2 diabetes[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1766.
[5]卢薇,欧亚萍,梁英梅.二甲双胍对2型糖尿病患者血HS-CRP、TNF-α、ICAM-1水平的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1768.
[6]刘红,罗蕾,高原.2型糖尿病大鼠近球小管Na+,K+-ATPase活性变化[J].陆军军医大学学报(原第三军医大学学报),2006,28(20):2062.
[7]贾贺堂,张素华,纪立农,等.北京地区早发糖尿病家系MODY5基因突变的筛查[J].陆军军医大学学报(原第三军医大学学报),2006,28(19):1952.
[8]杨孟雪,甘华,沈清,等.1, 25-(OH)2D3及LPS对2型糖尿病肾病尿毒症患者单核细胞维生素D受体表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(16):1731.
　Yang Mengxue,Gang Hua,Sheng Qing,et al.Effect of 1,25-(OH)2D3 and LPS on expression of vitamin D receptor in human monocytes incubated with sera from type 2 diabetes patients and diabetic nephropathy patients with uremia[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(17):1731.
[9]张学亮,冯晓丽,徐静,等.曲美他嗪对2型糖尿病患者血乳酸水平的影响[J].陆军军医大学学报(原第三军医大学学报),2005,27(24):2478.
[10]唐晓君,卢仙娥,张素华,等.LR 3 057位基因多态性与2型DM和肥胖及血脂的关系[J].陆军军医大学学报(原第三军医大学学报),2005,27(15):1596.
[11]杨静,张朝军,郑宏庭,等.胃转流手术治疗肥胖2型糖尿病短期疗效观察[J].陆军军医大学学报(原第三军医大学学报),2012,34(21):2222.
[12]杨建江,倪银星,何洪波,等.腹腔镜Roux-en-Y胃转流术对2型糖尿病患者术后近期血压的影响[J].陆军军医大学学报(原第三军医大学学报),2013,35(09):828.
　Yang Jianjiang,Ni Yinxing,He Hongbo,et al.Effects of laparoscopic Roux-en-Y gastric bypass on early postoperative blood pressure in patients with type 2 diabetes[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(17):828.
[13]蒙长远,陈志雄,官文飞,等.Roux-en-Y胃转流术对2型糖尿病大鼠肝脏AQP9的影响[J].陆军军医大学学报(原第三军医大学学报),2014,36(03):257.
　Meng Changyuan,Chen Zhixiong,Guan Wenfei,et al.Effects of Roux-en-Y gastric bypass on level of aquaporin-9 in type 2 diabetes rats[J].J Amry Med Univ (J Third Mil Med Univ),2014,36(17):257.

更新日期/Last Update: 2012-08-31