[1]郝静梅,韦红,张晓萍,等.产前使用地塞米松对坏死性小肠结肠炎新生鼠Toll样受体4表达的影响[J].第三军医大学学报,2012,34(02):145-149.
 Hao Jingmei,Wei Hong,Zhang Xiaoping,et al.Antenatal dexamethasone downregulates toll-like receptor 4 in neonatal rats with necrotizing enterocolitis[J].J Third Mil Med Univ,2012,34(02):145-149.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
34卷
期数:
2012年第02期
页码:
145-149
栏目:
论著
出版日期:
2012-01-30

文章信息/Info

Title:
Antenatal dexamethasone downregulates toll-like receptor 4 in neonatal rats with necrotizing enterocolitis
作者:
郝静梅韦红张晓萍刘玮
重庆医科大学附属儿童医院:儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地,新生儿诊治中心,儿科研究所
Author(s):
Hao Jingmei Wei Hong Zhang Xiaoping Liu Wei
Neonatal Diagnosis and Treatment Center, Pediatric Research Institute, Key Laboratory of Child Development and Disorders  Cofounded by Chongqing and Ministry of Education, Key Laboratory of Pediatrics of Chongqing,  Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children’s Hospital,  Chongqing Medical University, Chongqing, 400014, China
关键词:
坏死性小肠结肠炎地塞米松产前给药Toll样受体4新生鼠
Keywords:
necrotizing enterocolitis antenatal dexamethasone toll-like receptor 4 neonatal rat
分类号:
R722;R725.74;R977.1
文献标志码:
A
摘要:
目的      观察产前使用地塞米松(dexamethasone, DEX)对新生大鼠坏死性小肠结肠炎(necrotizing enterocolitis,NEC)发生率及肠道Toll样受体4(toll like receptor 4,TLR4)表达的影响,探讨其对NEC的保护作用及可能的机制。      方法        实验分为正常对照组、产前地塞米松干预组(DEX组)、产前生理盐水干预组(normal saline, NS组)3组,于孕16、17、18 d时DEX组孕鼠肌肉注射DEX 0.5 mg/(kg·d),NS组每天注射NS 0.3 ml。DEX组及NS组所生新生鼠建立NEC动物模型。正常对照组不予处理。于第5天处死新生鼠,观察近回盲部肠组织病理变化并做评分;免疫组化法检测TLR4蛋白的表达;其余小肠部分Q-PCR法检测TLR4 mRNA的表达。      结果        与NS组相比,DEX组新生鼠NEC发生率明显降低(P<0.01)。正常对照组未出现NEC症状,NS组新生鼠出现典型的NEC症状,DEX组NEC症状比NS组出现得更晚、更轻;与正常对照组相比,DEX组和NS组肠组织病理学评分及TLR4表达显著增高(P<0.05);与NS组相比,DEX组肠组织病理学评分及TLR4表达显著降低(P<0.05)。      结论        产前使用DEX对NEC大鼠具有保护作用,其机制可能是通过降低TLR4的表达从而发挥抗炎效应。
Abstract:
Objective        To observe the effect of antenatal dexamethasone (DEX) treatment on expression of toll-like receptor 4 (TLR4) in neonatal rats with necrotizing enterocolitis (NEC), and to explore its protective effect and potential mechanisms.       Methods        Twelve pregnant Sprague-Dawley (SD) rats were randomly divided into three groups with 4 rats in each group, including a normal control group (NC group), a DEX group and a normal saline group (NS group). On the gestational days 16, 17 and 18, rats of the DEX group and the NS group were intramuscularly injected with DEX [0.5 mg/(kg·d)] and normal saline (0.3 ml/d), respectively. All rats of the three groups were delivered spontaneously (term=22 d). Neonatal rats of the DEX group and NS group (except NC group) were selected to establish NEC models by artificial feeding, cold exposure after hypoxic-reoxygenation and LPS intragastric administration. General conditions of neonatal rats were observed in each group. All the neonatal rats were sacrificed on the 5th day after birth and proximal intestine tissues of rat ileocecal junction were obtained. Pathological changes of the tissues were examined by pathological sectioning and hematoxylin and eosin (HE) staining, and protein expression of TLR4 was detected by immunohistochemical method. Total RNA was extracted from the rest of intestinal tissues, and mRNA expression of TLR4 was detected by Quantitative Real-time PCR (Q-PCR).       Results         Incidence of NEC in the DEX group was lower than that in the NS group. Typical NEC symptoms were observed in rats of the NS group but not the NC group, while the symptoms were later and milder in rats of the DEX group. Histopathologic scores and TLR4 expressions of the DEX and NS groups were significantly higher than those of the NC group (P<0.05), and the two indicators of the DEX group were significantly lower than those of the NS group (P<0.05).       Conclusion        Antenatal DEX has a protective effect on rat model of NEC probably by downregulating TLR4 expression to exert an anti-inflammatory effect.

参考文献/References:

郝静梅, 韦红, 张晓萍, 等. 产前使用地塞米松对坏死性小肠结肠炎新生鼠Toll样受体4表达的影响[J].第三军医大学学报,2012,34(2):145-149.

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更新日期/Last Update: 2012-01-13