[1]陈洁,熊吉,陈潇迪,等.Calpain 2调节自噬相关基因ATG7的表达在非酒精性脂肪性肝病中的作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(20):2162-2165.
　Chen Jie,Xiong Ji,Chen Xiaodi,et al.Role of regulation of autophagy related gene 7 by Calpain 2 in non-alcoholic fatty liver disease in rats[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(20):2162-2165.
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Calpain 2调节自噬相关基因ATG7的表达在非酒精性脂肪性肝病中的作用(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第20期
页码:
2162-2165
栏目:
论著
出版日期:
2011-10-30

文章信息/Info

Title:
Role of regulation of autophagy related gene 7 by Calpain 2 in non-alcoholic fatty liver disease in rats
作者:
陈洁; 熊吉; 陈潇迪; 牟歌; 王军; 樊丽琳; 陈东风
第三军医大学大坪医院野战外科研究所消化内科
Author(s):
Chen Jie;  Xiong Ji;  Chen Xiaodi;  Mu Ge;  Wang Jun;  Fan Lilin;  Chen Dongfeng
Department of Gastroenterology, Institute of Surgery Research, Daping Hospital, Third Millitary Medical University, Chongqing, 400042, China
关键词:
非酒精性脂肪性肝病;  Calpain 2; 自噬相关基因7; 大鼠
Keywords:
non-alcoholic fatty liver disease; Calpain 2; autophagy related gene 7; rats
分类号:
R341;R394.3;R575.5
文献标志码:
A
摘要:
目的      明确Calpain 2及自噬相关基因7（autophagy related gene 7，ATG7）在非酒精性脂肪性肝病中的表达变化及意义。      方法      应用高脂饮食喂养SD大鼠建立非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)在体模型，并按时相点分为4、8、12周及16周组，同时设立正常饮食组为对照组，每组6只。检测各组大鼠血清ALT、AST、FFA水平，通过HE染色观察肝脏脂肪变情况，利用Real-time PCR及Western blot检测Calpain 2及ATG7的表达变化。      结果      ①HE染色提示肝脏脂肪变程度随高脂饮食喂养时间的延长而加重。NAFLD大鼠模型各时相点血清 ALT、 AST、 FFA水平均较对照组有不同程度的升高，在16周组时分别为（165.95±7.24 ）U/L、（249.52±4.20） U/L、（0.83±0.05）mmol/L,与对照组比较均有明显的升高（P<0.01）。②以正常饮食组为对照组，肝组织Calpain 2 mRNA相对表达量在高脂饮食喂养后开始上调，在16周时升高最为明显（9.83±0.85，P<0.01）；ATG7的mRNA相对表达量在4周组（0.82±0.02）即开始下降，在16周组（0.20±0.03）降至最低，与对照组比较降低显著(P<0.01)。③与对照组比较，Calpain 2蛋白相对表达水平与mRNA表达水平一致，4周组（2.32±0.45）时开始上调，16周组明显上升（9.87±1.20，P<0.01）。而ATG7的蛋白相对表达水平则随肝脏脂肪变性的进展明显下降，16周时降低最为明显（0.18±0.05，P<0.01)。      结论      脂肪肝发生过程中，Calpain 2的表达上调抑制了自噬相关基因ATG7的表达，进而减弱了自噬对细胞的保护作用，导致肝细胞的损伤加重，自噬可能参与了NAFLD的发生。
Abstract:
Objective      To investigate the expression and significance of autophagy related gene 7 (ATG7) and Calpain 2 in nonalcoholic fatty liver disease (NAFLD).       Methods      In vivo model of NAFLD was established in SD rats by high fat diet, while the rats fed with normal food were set as control group. The rats were killed at 4, 8, 12 and 16 weeks after feeding. Blood samples were collected to check serum aspartate transaminase (AST), alanine aminotransferase (ALT), and free fatty acid(FFA). Steatosis of liver tissues were observed by HE staining. The expression of Calpain 2 and ATG7 was detected by real-time PCR and Western blotting respectively for mRNA and protein levels.       Results      HE staining implicated that the degree of hepatic steatosis was increased with the time of high fat diet feeding. Compared with the control group, the serum contents of ALT, AST, and FFA in NAFLD rats were increased with different degree, and significantly increased at 16th week (165.95±7.24 U/L, 249.52±4.20 U/L, 0.83±0.05 mmol/L, respectively P<0.01). The relative expression of Calpain 2 at mRNA level was increased after high fat diet feeding and reached its peak at the 16th week, and was 9.83±0.85 fold higher as compared with the control group (P<0.01). While the relative expression of ATG7 began to decrease at the 4th week (0.82±0.02), and reached its lowest level at the 16th week (0.20±0.03, P<0.01) when compared with the control group. As with mRNA level, the protein level of Calpain 2 began to increase at the 4th week (2.32±0.45), and was 9.87±1.20 fold higher as the control group (P<0.01). While the expression of ATG7 at protein level was decreased with progress of steatosis, and was significantly reduced at 16th week (0.18±0.05, P<0.01).       Conclusion      The up-regulation of Calpain 2 inhibits the expression of ATG7, which further attenuates the cell protection through autophagy, and then induces injury of hepatocyte in NAFLD. Autophagy may take part in the incidence of NAFLD.

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　Mu Ge,Wang Xiaomin,Xiong Ji,et al.Dynamic expression and significance of PACS-2 in non-alcoholic fatty liver disease[J].J Amry Med Univ (J Third Mil Med Univ),2013,35(20):609.
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更新日期/Last Update: 2011-10-17