[1]倪志宇,丛斌,董玫,等.八肽胆囊收缩素对脂多糖诱导RAW 264.7细胞IL-1β、IL-6、IL-10表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(22):2366-2369.
　Ni Zhiyu,Cong Bin,Dong Mei,et al.Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(22):2366-2369.
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八肽胆囊收缩素对脂多糖诱导RAW 264.7细胞IL-1β、IL-6、IL-10表达的影响(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第22期
页码:
2366-2369
栏目:
论著
出版日期:
2011-11-30

文章信息/Info

Title:
Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells
作者:
倪志宇; 丛斌; 董玫; 马春玲; 李淑瑾; 文迪; 徐之璐
河北医科大学基础医学院法医学系，河北省法医学重点实验室
Author(s):
Ni Zhiyu;  Cong Bin;  Dong Mei;  Ma Chunling;  Li Shujin;  Wen Di;  Xu Zhilu
Department of Forensic Medicine, Hebei Key Laboratory of Forensic Medicine, College of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei Province, 050017, China
关键词:
胆囊收缩素; 脂多糖; RAW 264.7细胞; IL-1β; IL-6; IL-10
Keywords:
cholecystokinin;  lipopolysaccharides;  RAW 264.7 cell;  IL-1β;  IL-6;  IL-10
分类号:
R392-33;R392.12;R392.32
文献标志码:
A
摘要:
目的      研究脂多糖（lipopolysaccharide，LPS）诱导RAW 264.7细胞促炎性细胞因子IL-1β、IL-6及抗炎性细胞因子IL-10的动态变化规律及八肽胆囊收缩素（cholecystokinin octapeptide,CCK-8）对其表达的影响。      方法      LPS诱导RAW 264.7细胞不同时间（0、3、6、12、24、48 h），在各细胞因子表达高峰的时间点用ELISA及RT-PCR法检测不同剂量（10-10、10-9、10-8、10-7、10-6 mol/L）CCK-8对LPS诱导RAW 264.7细胞IL-1β、IL-6、IL-10蛋白及mRNA表达的影响。      结果      ①LPS可诱导RAW 264.7细胞IL-1β、IL-6、IL-10蛋白合成及mRNA表达增加(P<0.05,P<0.01)：各细胞因子蛋白的浓度分别于LPS刺激后6、24、24 h达到高峰[分别为（209.41±10.04）、（2 071.64±9.08）、（207.65±16.27）ng/L]；各细胞因子mRNA的表达分别于LPS刺激后3、24、24 h达到高峰[分别为（0.723±0.030）、（1.157±0.045）、(0.767±0.035)]。②CCK-8可剂量依赖性抑制IL-1β、IL-6的表达，并使IL-10表达进一步增加(P<0.01)，且孵育细胞24 h，CCK-8上调LPS诱导IL-10生成的幅度要大于孵育48 h。      结论      CCK-8通过抑制LPS诱导RAW 264.7细胞抑制促炎性细胞因子IL-1β、IL-6的表达和进一步增加抗炎性细胞因子IL-10的表达参与抗炎反应过程。
Abstract:
Objective      To observe the dynamic changes of proinflammatory cytokines IL-1β, IL-6 and anti-inflammatory cytokines IL-10 in LPS-induced RAW 264.7 cells and observe the effects of cholecystokinin octapeptide (CCK-8) on their expression.       Methods      After RAW 264.7 cells were stimulated with 1 mg/L LPS for 0, 3, 6, 12, 24 and 48 h respectively, the expression peak times of IL-1β, IL-6 and IL-10 were selected to investigate the effects of CCK-8 at the doses of 10-10, 10-9, 10-8, 10-7 and 10-6 mol/L respectively. Cytokines protein and mRNA expressions were assayed by ELISA and RT-PCR.       Results      The expression of IL-1β, IL-6 and IL-10 mRNA and protein were induced by LPS in RAW 264.7 cells in a time-dependent manner. CCK-8 inhibited LPS-induced IL-1β and IL-6 expression and augmented IL-10 expression in a dose-dependent manner. When the cells were treated for 24 h, CCK-8 augmentation resulted in higher LPS-induced IL-10 expression when compared with that after 48 hours’ treatment.       Conclusion      CCK-8 may participate in the anti-inflammatory response by inhibiting the expression of proinflammatory cytokines IL-1β and IL-6, meanwhile augmenting the expression of anti-inflammatory cytokine IL-10 induced by LPS.

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更新日期/Last Update: 2011-11-21