[1]张瑞瑞,刘磊,刘莹,等.依维莫司联合顺铂对胃癌细胞株SGC7901细胞周期的影响[J].第三军医大学学报,2011,33(18):1951-1955.
 Zhang Ruirui,Liu Lei,Liu Ying,et al.Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901[J].J Third Mil Med Univ,2011,33(18):1951-1955.
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依维莫司联合顺铂对胃癌细胞株SGC7901细胞周期的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第18期
页码:
1951-1955
栏目:
论著
出版日期:
2011-09-30

文章信息/Info

Title:
Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901
作者:
张瑞瑞刘磊刘莹孔庆兖
徐州医学院病理学教研室
Author(s):
Zhang Ruirui Liu Lei Liu Ying Kong Qingyan
Teaching and Research Section of Pathology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
关键词:
SGC7901细胞依维莫司细胞周期P21
Keywords:
SGC7901 cells RAD001 cell cycle P21
分类号:
R979.1;R735.2;R966
文献标志码:
A
摘要:
目的     探讨哺乳动物雷帕霉素靶蛋白特异性抑制剂依维莫司(everolimus,RAD001)对胃癌细胞株SGC7901细胞周期的影响、作用机制及其联合顺铂对SGC7901细胞抑制是否具有协同作用。     方法     体外培养SGC7901细胞,依维莫司单独及联合顺铂作用后,通过HE染色检测各组SGC7901细胞的形态学改变;流式细胞术检测细胞周期的改变;免疫细胞化学S-P法及Western blot检测细胞周期蛋白P21的表达。     结果     依维莫司能抑制SGC7901细胞增殖,且呈浓度剂量依赖性;联合用药对细胞生长抑制有协同效应;SGC7901细胞停滞在G0~G1期的细胞比例增加,其中联合组G0~G1期细胞比例最高(P<0.05)。药物干预48 h后SGC7901细胞中的P21蛋白表达上调,联合组明显高于相应单独用药组,差异有统计学意义(P<0.05)。     结论     依维莫司可以通过上调细胞周期蛋白P21的表达,阻滞胃癌SGC7901细胞周期进而抑制细胞增殖,联合用药对SGC7901细胞抑制具有协同效应。
Abstract:
Objective     To study the effect of a mTOR specific inhibitor, everolimus (RAD001), on cell cycle of gastric carcinoma cell line SGC7901 and the synergic inhibitory effect of combined RAD001 and cisplatin on proliferation of SGC7901 cells.      Methods     After SGC7901 cells were cultured in vitro and stained with HE, their morphology was observed after treated with RAD001 alone or in combination with cisplatin, and their cell cycle was assayed by flow cytometry. Expression of P21 in SGC7901 cells was detected by S-P immunecellchemistry and Western blotting, respectively.      Results     RAD001 could inhibit the proliferation of SGC7901 cells in a concentration-dependent manner. Combined RAD001 and cisplatin showed a synergic inhibitory effect on the proliferation of SGC7901 cells. The proportion of SGC7901 cells at G0-G1 was increased and significantly higher in combined RAD001 and cisplatin treatment group than in single RAD001 treatment group (P<0.05). The expression level of P21 in SGC7901 cells was significantly higher 48 h after combined RAD001 and cisplatin treatment than after single RAD001 treatment (P<0.05).      Conclusion     RAD001 can inhibit the proliferation of SGC7901 cells by up-regulating the expressions of P21 and blocking the cell cycle of SGC7901 cells. Combined RAD001 and cisplatin has a synergic inhibitory effect on the proliferation of SGC7901 cells.

参考文献/References:

张瑞瑞, 刘 磊, 刘莹, 等. 依维莫司联合顺铂对胃癌细胞株SGC7901细胞周期的影响[J].第三军医大学学报,2011,33(18):1951-1955.

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更新日期/Last Update: 2011-09-26