[1]邱钧,金俊余,孙建国,等.实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):984-987.
 Qiu Jun,Jin Junyu,Sun Jianguo,et al.Detection of miR-373* and miR-200c expression in breast cancer stem cells by real-time PCR and prediction of their target genes[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):984-987.
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实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第10期
页码:
984-987
栏目:
论著
出版日期:
2011-05-30

文章信息/Info

Title:
Detection of miR-373* and miR-200c expression in breast cancer stem cells by real-time PCR and prediction of their target genes
作者:
邱钧金俊余孙建国廖荣霞王欣欣陈正堂
第三军医大学新桥医院全军肿瘤诊治研究所;第三军医大学基础医学部医学英语教研室
Author(s):
Qiu Jun Jin Junyu Sun Jianguo Liao Rongxia Wang Xinxin Chen Zhengtang
Cancer Research Center,Xinqiao Hospital, Third Military Medical University, Chongqing, 400037; Department of Medical English, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
关键词:
miRNAs乳腺癌肿瘤干细胞非编码RNA
Keywords:
miRNAs breast cancer cancer stem cells non-coding RNAs
分类号:
R394.3;R730.3;R737.9
文献标志码:
A
摘要:
目的    分析miR-373*、miR-200c在乳腺癌干细胞(breast cancer stem cells,BCSCs)和MCF-7乳腺癌细胞株中的差异表达。    方法    Trizol法分别抽提乳腺癌干细胞和MCF-7细胞总RNA,实时定量PCR检测miRNAs 表达,对表达差异显著的miRNAs进行潜在靶基因预测。    结果    miR-373*在乳腺癌干细胞中较MCF-7细胞高表达(6.684±0.548),而miR-200c在乳腺癌干细胞中较MCF-7细胞低表达(0.345±0.031)。通过软件分析预测,miR-373*与TP53INP2、CASP8、EIF4A1、EEF1A1等基因均具有可能靶位点,miR-200c与TCF2、TDE2、SYVN1、PDCD10、RAP2C等基因均具有可能靶位点。    结论    miR-373*在乳腺癌干细胞中高表达,可能是一个潜在的癌基因。miR-200c在乳腺癌干细胞中低表达,可能是一个潜在的抑癌基因。
Abstract:
Objective    To analyze the differential expression of miR-373* and miR-200c in breast cancer stem cells (BCSCs) and breast cancer cell line MCF-7.     Methods    Total RNA of BCSCs and MCF-7 cells were extracted with Trizol. MicroRNA (miRNA) expression was detected by real-time PCR. The potential target genes of miRNAs that exhibited significant differential expression were predicted using software PicTar, miRanda, and Targetscan.     Results    Compared with those in MCF-7 cells, the expression levels of miR-373* and miR-200c were about 7 times higher (fold change=6.684±0.548) and about 3 times lower (fold change=0.345±0.031), respectively, in BCSCs. Prediction software analysis indicated that miR-373* had potential target sites in TP53INP2, CASP8, EIF4A1 and EEF1A1, and miR-200c had potential target sites in TCF2, TDE2, SYVN1, and some RAS oncogene family members.     Conclusion    In comparison with MCF-7 cells, BCSCs show up-regulated expression of miR-373*, which may be a potential oncogene, and down-regulated expression of miR-200c, which may be a potential anti-oncogene.

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更新日期/Last Update: 2011-05-12