[1]刘颖,陈幸华,张曦,等.人脐血源基质细胞对造血细胞归巢植入能力的影响[J].第三军医大学学报,2011,33(05):450-454.
 Liu Ying,Chen Xinghua,Zhang Xi,et al.Effect of hUCBDSCs on homing efficiency and engraftment capability of hematopoietic cells[J].J Third Mil Med Univ,2011,33(05):450-454.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第05期
页码:
450-454
栏目:
论著
出版日期:
2011-03-15

文章信息/Info

Title:
Effect of hUCBDSCs on homing efficiency and engraftment capability of hematopoietic cells
作者:
刘颖陈幸华张曦高蕾张诚冯一梅
第三军医大学新桥医院血液科
Author(s):
Liu YingChen XinghuaZhang XiGao LeiZhang ChengFeng Yimei
Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
人脐血基质细胞造血细胞归巢植入
Keywords:
human umbilical cord stromal cells hematopoietic cells homing engraftment
分类号:
R392.28; R331.14; R394.2
文献标志码:
A
摘要:
目的    探讨人脐血源基质细胞(human umbilical cord blood-derived stromal cells, hUCBDSCs)对造血细胞早期归巢及植入能力的影响。     方法      CCK-8法和Transwell法分别比较hUCBDSCs和人骨髓基质细胞(human bone marrow stromal cells, hBMSCs)对脐血单个核细胞(human umbilical cord blood-mononuclear cells, hUCB-MNCs)体外黏附及迁移能力的影响;RT-PCR法检测hUCBDSCs对促归巢相关因子的表达;60Coγ射线致死剂量辐照BABL/c小鼠后分别接受hUCB-MNCs单移植或hUCB-MNCs联合基质细胞共移植,流式细胞仪检测小鼠骨髓人源CD45+细胞植入率;追踪移植后CM-DiI标记的hUCB-MNCs体内迁移情况,并比较各组归巢率。    结果    hUCBDSCs和hBMSCs均能促进hUCB-MNCs的黏附,且迁移作用显著强于无基质细胞共培养对照(P<0.05);hUCBDSCs能表达一系列与造血归巢相关的黏附分子和细胞因子;hUCBDSCs在联合hUCB-MNCs共移植中能不同程度提高造血植入率,且当输注的hUCB-MNCs为低剂量(两种细胞按1〖DK〗∶1各移植2×106/只)时这种促进作用最为显著(P<0.01);CM-DiI标记的hUCB-MNCs在体内主要归巢至骨髓和脾脏,hUCBDSCs联合移植后48 h归巢率(43.49±3.06)%显著高于hBMSCs联合移植组[(31.99±7.26)%]和单移植组[(21.46±6.88)%],(P<0.05)。    结论     hUCBDSCs在促进造血细胞迁移、归巢和植入方面作用显著。
Abstract:
Objective     To investigate the effect of human umbilical cord blood-derived stromal cells (hUCBDSCs) on early homing efficiency and engraftment capability of hematopoietic cells.       Methods     CCK-8 method and Transwell method were utilized to compare the effects of hUCBDSCs and human bone marrow stromal cells (hBMSCs) on in vitro adhesion and migration capability of human umbilical cord blood-mononuclear cells (hUCB-MNCs). RT-PCR assay was employed to detect the expression of homing-associated factors in hUCBDSCs. BABL/c mice were irradiated by 60Co γ-ray at a lethal dose, and then hUCB-MNCs were transplanted into some of the BABL/c mice alone and into others in combination with hUCBDSCs or hBMSCs. The engraftment rates of human CD45+ cells in mouse bone marrow were measured by flow cytometry. The in vivo  migration of CM-DiI-labeled hUCB-MNCs after transplantation was traced, and homing rates were compared accordingly.     Results     Both hUCBDSCs and hBMSCs promoted the adhesion of hUCB-MNCs and significantly enhance the migration of hUCB-MNCs (P<0.05). The hUCBDSCs expressed a series of homing-associated adhesion molecules and cytokines, and increased the engraftment rate of hUCB-MNCs, particularly when hUCB-MNCs were transplanted at a low dose (2×106 hUCB-MNCs and 2×106 hUCBDSCs per mouse) (P<0.01). The CM-DiI-labeled hUCB-MNCs mainly homed to the bone marrow and spleen in vivo. The hUCB-MNCs showed a higher homing rate when transplanted in combination with hUCBDSCs than when transplanted alone or in combination with hBMSCs at 48 h after transplantation [(43.49±3.06)% vs (31.99±7.26)% and (21.46±6.88)%] (P<0.05).      Conclusion      hUCBDSCs can effectively promote the migration, homing, and engraftment of hUCB-MNCs.

参考文献/References:

刘颖, 陈幸华, 张曦, 等. 人脐血源基质细胞对造血细胞归巢植入能力的影响[J].第三军医大学学报,2011,33(5):450-454.

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更新日期/Last Update: 2011-03-04