[1]张旋,周政,刘俊,等.Caveolin-1与兔蛛网膜下腔出血模型基底动脉平滑肌增殖的关系[J].陆军军医大学学报(原第三军医大学学报),2011,33(08):830-834.
 Zhang Xuan,Zhou Zheng,Liu Jun,et al.Relationship between caveolin-1 and proliferation of basilar artery smooth muscle in rabbit model of subarachnoid hemorrhage[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(08):830-834.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第08期
页码:
830-834
栏目:
论著
出版日期:
2011-04-30

文章信息/Info

Title:
Relationship between caveolin-1 and proliferation of basilar artery smooth muscle in rabbit model of subarachnoid hemorrhage
作者:
张旋周政刘俊冯宝海张建忠黄巍
第三军医大学新桥医院神经外科
Author(s):
Zhang Xuan Zhou Zheng Liu Jun Feng Baohai Zhang Jianzhong Huang Wei
Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China
关键词:
蛛网膜下腔出血小窝蛋白-1血管平滑肌细胞增殖
Keywords:
subarachnoid hemorrhage Caveolin-1 vascular smooth muscle cell proliferation
分类号:
R743.35;R322.12;R329.26
文献标志码:
A
摘要:
目的    观察兔蛛网膜下腔出血(subarachnoid hemorrhage,SAH)模型基底动脉小窝蛋白-1(Caveolin-1)和血小板源性生长因子(platelet-derived growth factor,PDGF)随时间表达变化情况,分析caveolin-1与动脉中膜平滑肌细胞增殖的关系。    方法    采用随机数字表法将76只新西兰大白兔完全随机分为空白对照组、假手术组和SAH组,空白组(各8只)不施加任何因素干扰,SAH组通过枕大池2次注血模拟脑血管痉挛病理过程,假手术组以生理盐水代替自体动脉血。于2次注血完毕后第1、3、5、7、14天取材,进行基底动脉组织学观察;RT-PCR、Western blot检测caveolin-1及PDGF mRNA转录及蛋白表达的变化规律。    结果    SAH组基底动脉中膜滑肌层第7天增厚最为明显(39.92±4.07)μm,较sham组(10.78±2.14)μm显著增厚(P<0.01),第14天恢复正常;SAH组caveolin-1 mRNA转录水平上调,第5天达峰(63.41±4.47)%,较sham组(11.75±1.84)%显著上调(P<0.01),蛋白表达则持续下降,第7天(13.78±0.59)%较sham组(37.63±0.85)%显著下调(P<0.01);SAH组PDGF mRNA转录水平第3天(64.45±4.00)%较sham组(11.13±1.20)%显著上调(P<0.01),蛋白表达于第7天达峰(40.67±0.81)%,较sham组(15.42±1.00)%显著上调(P<0.01);Cavelin-1蛋白表达与基底动脉中膜平滑肌层厚度呈显著负相关(r=-0.89,P<0.01)。    结论    Caveolin-1蛋白在SAH后脑血管平滑肌细胞增殖过程中持续下调,可能是抑制血管痉挛平滑肌增殖反应的潜在靶点。
Abstract:
Objective    To investigate the expression changes of caveolin-1 and platelet-derived growth factor (PDGF) in the basilar artery of the rabbit model of subarachnoid hemorrhage (SAH) over time and to analyze the relationship between caveolin-1 expression and the proliferation of vascular smooth muscle cells (VSMCs) in basilar artery.     Methods    Seventy-six New Zealand rabbits were randomly divided into a normal group, a sham group, and SAH groups (n=5). Double injection of autologous arterial blood into the cisterna magna was employed to simulate cerebral vasospasm (CVS) in the SAH groups. Sterile saline was used in place of the autologous arterial blood in the sham group. Basilar artery specimens were collected on the 1st, 3rd, 5th, 7th, and 14th days after double injection. Then, the tissues of the specimens were observed using pathological sections, and the mRNA and protein expressions of caveolin-1 and PDGF were investigated through RT-PCR and Western blotting.     Results    The thickness of vascular smooth muscle layer of basilar artery in the SAH groups increased gradually and peaked on the 7th day, significantly increased as compared with that in the sham group [(39.92±4.07)μm vs (10.25±1.83)μm, P<0.01], and then decreased to a normal value on the 14th day. The mRNA expression of caveolin-1 in the SAH groups peaked on the 5th day, significantly increased as compared with that in the sham group [(63.41±4.47)% vs (11.75±1.84)%, P<0.01]. The protein expression of caveolin-1 in the SAH groups decreased to the lowest point on the 7th day, significantly decreased as compared with that in the sham group [(13.78±0.59)% vs (37.63±0.85)%, P<0.01]. The mRNA expression of PDGF in the SAH groups peaked on the 3rd day, significantly increased as compared with that in the sham group [(64.45±4.00)% vs (11.13±1.20)%, P<0.01]. The protein expression of PDGF in the SAH groups peaked on the 7th day, significantly increased as compared with that in the sham group [(40.67±0.81)% vs (15.42±1.00)%, P<0.01]. The protein expression of caveolin-1 was significantly and negatively correlated with the thickness of vascular smooth muscle layer of basilar artery (r=-0.89, P<0.01).     Conclusion    The protein expression of caveolin-1 is down-regulated continuously during the proliferation of VSMCs in basilar artery. Caveolin-1 may be a potential inhibitor for proliferation of VSMCs in CVS.

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更新日期/Last Update: 2011-04-13