[1]李琪佳,郭静,甘洪全,等.成骨细胞及血管内皮细胞复合同种异体颗粒骨治疗大鼠股骨头坏死[J].第三军医大学学报,2011,33(06):591-595.
 Li Qijia,Guo Jing,Gan Hongquan,et al.Osteoblasts and vascular endothelial cells combined with allogeneic morselized bone graft for treating rat osteonecrosis of the femoral head[J].J Third Mil Med Univ,2011,33(06):591-595.
点击复制

成骨细胞及血管内皮细胞复合同种异体颗粒骨治疗大鼠股骨头坏死(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第06期
页码:
591-595
栏目:
论著
出版日期:
2011-03-30

文章信息/Info

Title:
Osteoblasts and vascular endothelial cells combined with allogeneic morselized bone graft for treating rat osteonecrosis of the femoral head
作者:
李琪佳郭静甘洪全宋会平白俊清王志强
华北煤炭医学院:实验中心,附属医院骨科
Author(s):
Li Qijia Guo JingGan HongquanSong Huiping Bai Junqing Wang Zhiqiang
Laboratory Center, Department of Orthopaedics, Affiliated Hospital, North China Coal Medical Collage, Tangshan, Hebei Province, 063000, China
关键词:
骨髓基质干细胞成骨细胞血管内皮细胞同种异体骨股骨头坏死
Keywords:
bone marrow stromal stem cells osteoblast vascular endothelial cell allogeneic bone osteonecrosis of the femoral head
分类号:
R-332;R618.805;R329.24
文献标志码:
A
摘要:
目的    探讨骨髓基质干细胞(bone marrow stromal stem cells,BMSCs)来源的大鼠同种异体成骨细胞(osteoblast, OB)及血管内皮细胞(vascular endothelial cells, VEC)与同种异体颗粒骨复合后治疗大鼠创伤性股骨头坏死(osteonecrosis of the femoral head, ONFH)的可能性。    方法     36只6月龄SD大鼠,分为模型组、同种骨组及OB+VEC+同种骨组(n=12)。同时取4周龄SD大鼠BMSCs体外分离培养,取第3代细胞复合同种颗粒骨;制备大鼠创伤性股骨头坏死动物模型(圆韧带离断法)并分别在术后1、2、4、6周处死动物并取材。扫描电镜观察两种细胞在支架上的生长情况;免疫细胞化学技术检测诱导后血管内皮细胞CD34 、CD31的表达; 碱性磷酸酶(ALP)染色及Ⅰ型胶原免疫细胞化学染色鉴定成骨细胞;5-溴脱氧尿嘧啶(5-bromo-2-deoxy Uridine, BrdU)体外标记成骨细胞及内皮细胞,动物体内示踪两种细胞动力学。光镜观察病理形态改变;通过骨形态计量学参数计算新骨生成量,包括骨小梁面积百分率(percent trabecular area, %Tb.Ar)、骨小梁厚度(trabecular bone thickness, Tb.Th)、骨小梁数量(trabecular bone number, Tb.N)、骨小梁分离度(trabecular bone separation degree, Tb.Sp)。     结果      BMSCs诱导来源的成骨细胞其ALP染色及Ⅰ型胶原免疫组化染色均呈阳性反应;血管内皮细胞表达了其标志物CD31、CD34。两种细胞与同种骨有良好的组织细胞相容性,细胞在其表面及壁内平铺、伸展、生长状态良好。OB+VEC+同种骨组新骨生成量均多于模型组及同种骨组,各项新骨形成参数均高于模型组及同种骨组(P<0.01)。     结论    OB+VEC+同种骨支架材料在治疗实验性大鼠ONFH中其促成骨能力优于单纯同种异体颗粒骨。
Abstract:
Objective     To study the possibility of bone marrow stromal stem cells (BMSCs)-derived allogeneic osteoblasts (OBs)/vascular endothelial cells (VECs) combined with allogeneic morselized bone grafts for treating rat traumatic osteonecrosis of the femoral head (ONFH).     Methods      Thirty-six six-month-old SD rats were divided into three groups: model group, allogeneic bone group, and OB+VEC+allogeneic bone group, each including 12 rats. BMSCs, extracted from 4-week-old SD rats, were cultured in vitro, and the obtained third-generation cells were combined with allogeneic morselized bone. An animal model for rat traumatic ONFH was built through removing round ligaments of femoral heads, and the animals were sacrificed in the first, second, fourth, and sixth weeks after implanting OB+VEC+allogeneic morselized bone or allogeneic morselized bone to collect the femoral heads. The growth of OBs and VECs on allogeneic morselized bone was observed with a scanning electronic microscope. The expression of CD34 and CD31 in VECs was detected through an immunocytochemical method. OBs were identified through alkaline phosphatase (ALP) staining and collagen type-Ⅰ immunocytochemical staining. OBs and VECs were labeled with 5-bromo-2-deoxy uridine (BrdU) in vitro, so as to trace the kinetics of OBs and VECs in vivo. Pathological morphology changes were observed with an optical microscope. New bone formation was calculated according to bone histomorphometry parameters, including percent trabecular area (%Tb.Ar), trabecular bone thickness (Tb.Th), trabecular bone number (Tb.N), and trabecular bone separation degree (Tb.Sp).     Results    ALP staining and collagen type-Ⅰ immunohistochemical staining of the BMSC-derived OBs gave positive results. The VECs expressed CD31 and CD34. The OBs and VECs exhibited excellent biocompatibility with the allogeneic bone, and were in good spreading, extension, and growth conditions on its surfaces. The OB+VEC+allogeneic bone group accomplished more new bone formation than the model group and allogeneic bone group, and each new bone formation parameter of the OB+VEC+allogeneic bone group was higher than those in the model group and allogeneic bone group (P<0.01).     Conclusion     The OB+VEC+allogeneic bone composite has higher ability to promote new bone formation in treatment of rat traumatic ONFH than allogeneic morselized bone alone.

参考文献/References:

李琪佳, 郭静, 甘洪全, 等. 成骨细胞及血管内皮细胞复合同种异体颗粒骨治疗大鼠股骨头坏死[J].第三军医大学学报,2011,33(6):591-595.

相似文献/References:

[1]陈莉丽,黄玫,雷利红,等.OPG/RANKL/RANK系统参与牙槽骨吸收及重建过程作用初探[J].第三军医大学学报,2013,35(04):288.
 Chen Lili,Huang Mei,Lei Lihong,et al.Role of OPG/RANKL/RANK system during alveolar bone resorption and remodeling[J].J Third Mil Med Univ,2013,35(06):288.
[2]张纲,亓连军,谭颖徽,等.bFGF-PLA-Ns对体外培养成骨细胞增殖、分化及矿化的影响[J].第三军医大学学报,2007,29(21):2073.
 ZHANG Gang,QI Lian-jun,TAN Ying-hui,et al.Effect of basic fibroblast growth factor-polylactide sustained release nanospheres on proliferation, differentiation and mineralization of osteoblasts in vitro[J].J Third Mil Med Univ,2007,29(06):2073.
[3]黄爱文,金丹,裴国献,等.复合PRP的可注射型组织工程骨修复兔桡骨缺损的实验研究[J].第三军医大学学报,2007,29(14):1374.
 HUANG Ai-wen,JIN Dan,PEI Guo-xian,et al.Injectable tissue-engineered bone made of autologous platelet-rich plasma, fibrin glue and BMSCs for repairing segmental radial defect in rabbits[J].J Third Mil Med Univ,2007,29(06):1374.
[4]黄宏,朱方强,孙宏振,等.脂肪来源干细胞体外成骨诱导和成脂诱导分化[J].第三军医大学学报,2008,30(13):1219.
 HUANG Hong,ZHU Fang-qiang,SUN Hong-zhen,et al.Differentiation of swine adipose tissue-derived stromal cells into adipocytes and osteoblasts in vitro[J].J Third Mil Med Univ,2008,30(06):1219.
[5]杨华,张小明,邵阳,等.SPIO标记大鼠骨髓基质干细胞及体外磁共振成像研究[J].第三军医大学学报,2008,30(17):1626.
 YANG Hua,ZHANG Xiao-ming,SHAO Yang,et al.Superparamagnetic iron oxide labeled mesenchymal stem cells and their magnetic resonance imaging in vitro[J].J Third Mil Med Univ,2008,30(06):1626.
[6]冯正平,邓华聪,姜蓉,等.p38MAPK基因RNAi慢病毒载体的构建及在MC3T3-E1细胞的表达[J].第三军医大学学报,2010,32(15):1598.
 Feng Zhengping,Deng Huacong,Jiang Rong,et al.Construction of recombinant lentiviral vector of siRNA for p38MAPK gene and its expression in MC3T3-E1 cells[J].J Third Mil Med Univ,2010,32(06):1598.
[7]陈允嘉,王豫蓉,邹林洪,等.放线共生放线杆菌培养上清对成骨细胞分化及间隙连接通讯的影响[J].第三军医大学学报,2010,32(10):1059.
 Chen Yunjia,Wang Yurong,Zou Linhong,et al.Actinobacillus actinomycetemcomitans induces differentiation and gap junctional intercellular communication in cultured osteoblasts[J].J Third Mil Med Univ,2010,32(06):1059.
[8]彭吾训,王蕾,邓进,等.一种组织工程骨修复兔股骨头坏死模型的效果评价[J].第三军医大学学报,2010,32(07):661.
 Peng Wuxun,Wang Lei,Deng Jin,et al.A tissue engineering bone to repair femoral head necrosis[J].J Third Mil Med Univ,2010,32(06):661.
[9]王胜国,周力,陈扬熙,等.不同强度静磁场对成骨细胞细胞内钙离子浓度的影响[J].第三军医大学学报,2010,32(23):2515.
 Wang Shengguo,Zhou Li,Chen Yangxi,et al.Effect of static magnetic field at different strengths on Ca2+ concentration in rat primarily cultured osteoblasts[J].J Third Mil Med Univ,2010,32(06):2515.
[10]付建华,赵曼,杜桂英,等.应用骨髓干细胞和自制胶原-壳聚糖构建组织工程心瓣膜[J].第三军医大学学报,2011,33(02):152.
 Fu Jianhua,Zhao Man,Du Guiying,et al.Construction of tissue engineering heart valve with rabbit bone mesenchymal stem cells and self-made collage-chitosan complex scaffold[J].J Third Mil Med Univ,2011,33(06):152.

更新日期/Last Update: 2011-03-15