[1]崔浩,张超,刘涛,等.上调基因-4在结肠癌中的表达及临床意义[J].第三军医大学学报,2011,33(01):33-35.
 Cui Hao,Zhang Chao,Liu Tao,et al.Expression of up-regulated gene-4 in colon cancer and its clinicopathologic significance[J].J Third Mil Med Univ,2011,33(01):33-35.
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上调基因-4在结肠癌中的表达及临床意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第01期
页码:
33-35
栏目:
论著
出版日期:
2011-01-15

文章信息/Info

Title:
Expression of up-regulated gene-4 in colon cancer and its clinicopathologic significance
作者:
崔浩张超刘涛王攀徐建华
第三军医大学西南医院全军普通外科中心,微创胃肠外科中心
Author(s):
Cui Hao  Zhang Chao Liu Tao Wang Pan Xu Jianhua
Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
上调基因-4结肠癌RT-PCR免疫组化
Keywords:
up-regulated gene-4 colon cancer RT-PCR immunohistochemistry
分类号:
R730.23; R730.43; R735.35
文献标志码:
A
摘要:
目的  探讨上调基因-4 (up-regulated gene-4,URG-4)在结肠癌及其癌旁组织中的表达及临床意义。  方法   收集2010年1-6月本院手术切除的82例结肠癌、癌旁组织样本及石蜡切片标本,用RT-PCR、免疫组化检测URG-4基因的转录和表达情况,分析其与临床病理参数的关系。  结果   结肠癌组织中的URG-4 mRNA阳性率为85.37%(70/82),显著高于癌旁组织中的36.59%(30/82)(P<0.01)。URG-4蛋白在结肠癌中的表达率为70.73%(58/82),癌旁组织为24.39%(20/82),两组比较差异非常显著(P<0.01);肿瘤组织中URG-4的表达与患者的年龄、性别、肿瘤的分化程度、有无远处转移无明显相关性(P>0.05),而与浸润深度及淋巴结转移显著相关 (P<0.01)。  结论   与癌旁组织相比URG-4在结肠癌中高表达,并且URG-4表达强度与肿瘤浸润深度和淋巴结转移呈正相关,提示其可能在结肠癌的发生、发展中起重要作用。
Abstract:
Objective  To investigate the expression of up-regulated gene-4 (URG-4) in colon cancer tissues and adjacent non-cancerous tissues and its clinicopathologic significance.   Methods  Paraffin-embedded and liquid-nitrogen-preserved specimens of colon cancer tissues and adjacent non-cancerous tissues were prepared from 82 colon cancer patients in our hospital from January to June in 2010. Expression of URG-4 mRNA and protein was detected by reverse transcription PCR (RT-PCR) and immunohistochemical staining, and its correlation with clinicopathologic features was analyzed.   Results  The positive rate of URG-4 mRNA expression in colon cancer tissues (85.73%, 70/82) was significantly higher than that in adjacent non-cancerous tissues (36.59%, 30/82) (P<0.01). The expression rate of URG-4 protein in colon cancer tissues (70.73%, 58/82) was significantly higher than that in adjacent non-cancerous tissues (24.39%, 20/82) (P<0.01). In addition, the expression of URG-4 protein was positively related to tumor infiltration depth and lymph node metastasis (P<0.01), while no correlation was found between the expression of URG-4 protein and age, gender, tumor differentiation and distant metastasis (P>0.05).   Conclusion  The expression of URG-4 mRNA and protein in colon cancer tissues is significantly higher than that in adjacent non-cancerous tissues. Besides, it is positively correlated with tumor infiltration depth and lymph node metastasis in colon cancer. The results indicate that URG-4 plays an important role in colon cancer development.

参考文献/References:

崔浩, 张超, 刘涛, 等. 上调基因-4在结肠癌中的表达及临床意义[J].第三军医大学学报,2011,33(1):33-35.

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更新日期/Last Update: 2011-01-11