[1]任俊伟,杨琴,范层层,等.白藜芦醇减轻脑缺血再灌注损伤及其机制[J].第三军医大学学报,2010,32(24):2586-2590.
 Ren Junwei,Yang Qin,Fan Cengceng,et al.Resveratrol alleviates cerebral ischemia-reperfusion injury in rats[J].J Third Mil Med Univ,2010,32(24):2586-2590.
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白藜芦醇减轻脑缺血再灌注损伤及其机制(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第24期
页码:
2586-2590
栏目:
论著
出版日期:
2010-12-30

文章信息/Info

Title:
Resveratrol alleviates cerebral ischemia-reperfusion injury in rats
作者:
任俊伟杨琴范层层陈娜
重庆医科大学附属第一医院神经内科
Author(s):
Ren Junwei Yang Qin Fan Cengceng Chen Na
Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
关键词:
白藜芦醇脑缺血再灌注氧化应激Nrf2HO-1
Keywords:
resveratrolcerebral ischemia reperfusionoxidative stressNrf2HO-1
分类号:
R282.71;R743.31;R965
文献标志码:
A
摘要:
目的  研究白藜芦醇(Res)对脑缺血再灌注损伤的保护作用及可能的机制。  方法  线栓法制作大鼠大脑中动脉栓塞(middle cerebral artery occlusion, MCAO)模型,缺血2 h再灌注。将76只大鼠分成假手术组(S组)、缺血再灌注组(I/R组)、Res低剂量组(15 mg/kg,I/R+R1组)和Res高剂量组(30 mg/kg,I/R+R2组),每组19只。再灌注24 h,采用TTC法测量脑梗死体积,化学比色法测定血清及脑组织中MDA含量,RT-PCR及Western blot检测Nrf2、HO-1基因和蛋白表达,HE染色观察脑组织的病理改变。  结果  与I/R组比较,Res能明显缩小脑梗死体积[I/R组(42.67±2.51)% vs I/R+R1组(30.53±1.45)%、I/R+R2组(20.27±2.11)%,P<0.01],降低血清中MDA的含量[I/R组(9.29±0.42) nmol/ml vs I/R+R1组(4.03±0.08) nmol/ml、I/R+R2组(2.68±0.27)nmol/ml,P<0.01],及脑组织中MAD含量[I/R组(1.40±0.02)nmol/mg vs IR+R1组(1.19±0.07)nmol/mg、IR+R2组(0.85±0.03)nmol/mg,P<0.01]。显著上调Nrf2、HO-1基因和蛋白的表达(P<0.05),改善脑组织的病理变化。  结论  Res对局灶性脑缺血再灌注损伤有良好的保护作用,其机制可能是通过激活Nrf2/ARE信号通路进而增强抗氧化蛋白HO-1表达实现的。
Abstract:
Objective  To investigate the protective effect of resveratrol (Res) on cerebral ischemia and reperfusion (I/R) injury and its possible mechanism.   Methods  A total of 76 SD rats were randomized into 4 groups, that is, sham-operation group, I/R group, low dose of Res (15 mg/kg, I/R﹢R1 group) and high dose of Res (30 mg/kg, I/R﹢R2 group). Res at corresponding dose was injected to the rats peritoneally once per day for 7 d. And then middle cerebral artery occlusion (MCAO) model in rats was established by intraluminal thread block for 2 h and reperfusion thereafter. After 24 hours’ reperfusion, the content of MDA in the serum and brain tissue was determined with chemical colorimetry. 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to measure infarct area of brain tissue, and RT-PCR and Western blot analysis were employed the expressions of Nrf2 and HO-1 at mRNA and protein levels. Pathological changes of brain tissue was observed with HE staining.   Results  Res reduced the infarct areas by (30.53±1.45)% in I/R+R1 group and (20.27±2.11)% in I/R+R2 group when compared with I/R group [(42.67±2.51)%, P<0.01], decreased the contents of MDA in the serum (4.03±0.08 and 2.68±0.27 vs 9.29±0.42 nmol/ml, P<0.01) and brain tissue (1.19±0.07 and 0.85±0.03 vs 1.40±0.02 nmol/mg, P<0.01), significantly increased the protein and mRNA expression of both Nrf2 and HO-1 (P<0.05),and improved the pathological changes in brain tissue.   Conclusion  Res exerts protective effect on cerebral I/R injury in rats. The mechanism may imply the activation of Nrf2-ARE signaling pathway and thus enhance expression of antioxidant HO-1.

参考文献/References:

任俊伟, 杨琴, 范层层, 等. 白藜芦醇减轻脑缺血再灌注损伤及其机制[J].第三军医大学学报,2010,32(24):2586-2590.

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更新日期/Last Update: 2010-12-22