[1]明佳,袁侨英,周艳,等.体外LPS诱导血管内皮细胞通透性改变及其与连接蛋白43的关系[J].第三军医大学学报,2010,32(24):2582-2585.
 Ming Jia,Yuan Qiaoying,Zhou Yan,et al.Effect of LPS on vascular permeability of vascular endothelial cells and role of connexin43 in the process[J].J Third Mil Med Univ,2010,32(24):2582-2585.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第24期
页码:
2582-2585
栏目:
论著
出版日期:
2010-12-30

文章信息/Info

Title:
Effect of LPS on vascular permeability of vascular endothelial cells and role of connexin43 in the process
作者:
明佳袁侨英周艳苟元彬刘良明
第三军医大学西南医院:乳腺疾病中心,老年科;第三军医大学大坪医院野战外科研究所:第六研究室,第二研究室,创伤、烧伤与复合伤国家重点实验室
Author(s):
Ming Jia Yuan Qiaoying Zhou Yan Gou Yuanbin Liu Liangming
Breast Disease Center, Department of Geriatrics, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Department 6, Department 2, State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China
关键词:
脓毒性休克脂多糖血管通透性血管内皮细胞连接蛋白43
Keywords:
septic shockLPSvascular permeabilityvascular endothelial cellconnexin 43
分类号:
R322.12;R363.21;R631.1
文献标志码:
A
摘要:
目的  研究缝隙连接蛋白(connexin,Cx) 43在脂多糖(lipopolysaccharide,LPS)诱导的大鼠血管内皮细胞(vascular endothelial cell,VEC)通透性中的表达变化及其意义。  方法  以1、2、3、4 μg/ml  LPS刺激原代培养的大鼠VEC,观察作用15、30 min,1、2、3、4、5、6、7 h后VEC增殖活力和通透性的变化; 2 μg/ml  LPS刺激VEC后,采用RT-PCR和Western blot等方法检测Cx43的表达变化;分析VEC通透性和Cx43表达之间的相关性。  结果  1、2 μg/ml的LPS对VEC增殖活力无明显影响,3、4 μg/ml的LPS明显降低了VEC的增殖(P<0.01);2 μg/ml的LPS能够较好地模拟脓毒性休克的血管高通透性(P<0.01);用此浓度的LPS刺激VEC后Cx43的mRNA和蛋白表达均逐渐降低(P<0.01);Cx43的表达与LPS引起的VEC通透性改变呈负相关关系(r=-0.877,P<0.01)。  结论  Cx43可能参与了脓毒性休克后VEC通透性的调节;Cx43可能具有抑制脓毒性休克后血管内膜通透性的作用。
Abstract:
Objective  To investigate the expression and significance of connexin43 (Cx43) in the increased vascular permeability induced by LPS.   Methods  Rat vascular endothelial cells (VECs) were primary isolated from normal SD rats’ superior mesenteric arteries (SMAs), and then cultured with treatment of 1, 2, 3 or 4 μg/ml LPS for 15 and 30 min, and 1, 2, 3, 4, 5, 6 or 7 h. Cell vitality and permeability were determined with MTT assay and Transwell chamber technique respectively. The mRNA and protein expression of Cx43 were determined in the cells after 2 μg/ml LPS treatment.   Results  LPS at 1 and 2 μg/ml didn’t change the cell vitality, while 3 and 4 μg/ml LPS diseased it obviously (P<0.01). LPS of 2 μg/ml increased the cell permeability (P<0.01). The expression of Cx43 at mRNA and protein levels was gradually decreased after 2 μg/ml LPS treatment. VECs’ permeability and the expression of Cx43 had a negative correlationship.   Conclusion  Cx43 is involved in the regulation of vascular permeability following septic shock. Cx43 may be used to attenuate vascular permeability in septic shock.

参考文献/References:

明佳, 袁侨英, 周艳, 等. 体外LPS诱导血管内皮细胞通透性改变及其与连接蛋白43的关系[J].第三军医大学学报,2010,32(24):2582-2585.

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更新日期/Last Update: 2010-12-22