[1]谢会霞,郝进.雷公藤内酯醇通过p38MAPK信号通路诱导Hut102细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2010,32(19):2109-2111.
 Xie Huixia,Hao Jin.Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(19):2109-2111.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第19期
页码:
2109-2111
栏目:
论著
出版日期:
2010-10-15

文章信息/Info

Title:
Triptolide induces apoptosis of Hut102 cells through p38MAPK pathway
作者:
谢会霞郝进
重庆医科大学附属第二医院皮肤科
Author(s):
Xie Huixia Hao Jin
Department of Dermatology, Second Affiliated Hospital, Chongqing Medical University, Chongqing,400010, China
关键词:
雷公藤内酯醇蕈样肉芽肿Hut102p38MAPK细胞凋亡
Keywords:
triptolide mycosis fungoides Hut102 p38MAPK apoptosis
分类号:
R285.5; R730.23; R733.1
文献标志码:
A
摘要:
目的 探讨雷公藤内酯醇(triptolide,TP)诱导皮肤T细胞淋巴瘤蕈样肉芽肿细胞株Hut102的凋亡及其机制。 方法   体外培养的Hut102细胞与12.5、25、50、100 nmol/L雷公藤内酯醇共同孵育,分别在24、48、72 h用MTT法检测Hut102细胞的增殖抑制率,Annexin V-FITC/PI 双染流式细胞术分析细胞凋亡,Western blot检测Hut102细胞中磷酸化p38丝裂原活化蛋白激酶(p-p38)、caspase-3蛋白的表达水平。 结果   不同浓度雷公藤内酯醇(12.5、25、50、100 nmol/L)作用Hut102细胞24 h后的增殖抑制率分别为8.67%、30.02%、52.54%、59.62%。Annexin V-FITC/PI 双染流式细胞术检测经25、50、100 nmol/L雷公藤内酯醇处理24 h后Hut102细胞凋亡率分别为9.19%、21.49%、26.34%,当50 nmol/L雷公藤内酯醇组预先用p38MAPK特异性抑制剂SB203580干预后,Hut102细胞凋亡率下降为14.35%。不同浓度雷公藤内酯醇(50、100 nmol/L)处理Hut102细胞后p38、caspase-3蛋白的表达被激活,同样50 nmol/L雷公藤内酯醇组也预先用p38MAPK特异性抑制剂SB203580干预,结果p-p38的表达降低,caspase-3激活被抑制。 结论   雷公藤内酯醇可以抑制Hut102细胞的增殖,其作用机制可能是通过激活p38MAPK信号通路使部分Hut102细胞产生凋亡。
Abstract:
Objective   To study the effect of triptolide on apoptosis of human cutaneous T-cell lymphoma Hut102 cells and its mechanism.  Methods   Cutaneous T-cell lymphoma cell line Hut102 was co-cultured with triptolide solution at the terminal concentration of 12.5, 25, 50 and 100 nmol/L, respectively, in 96 well plates.At 24,48,and 72 h after co-culture,inhibitory effect of triptolide on Hut102 cells was detected by MTT assay, apoptosis rate of Hut102 cells was assessed by Annexin V-FITC/PI, and expression of phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK ) and caspase-3 was determined by Western blot analysis.  Results   Triptolide inhibited the proliferation of Hut102 cells in a concentration-and time-dependent manner. Annexin V-FITC/PI showed that the apoptotic rate of Hut102 cells was 9.19%,21.49% and 26.34%, respectively, in 24 h after they were treated with triptolide at the concentration of 25, 50 and 100 nmol/L,  respectively.However,the apoptosis rate of Hut102 cells was decreased by 14.35% after they were interfered with the p38MAPK inhibitor (SB203580). The expression of p-p38MAPK and caspase-3 was activated in Hut102 cells by different concentrations of triptolide (50 and 100 nmol/L). However, the expression of phosphorylated p38MAPK was decreased and the activity of caspase-3 was inhibited by triptolide at the concentration of 50 nmol/L interfered with SB203580.  Conclusion   Triptolide can inhibit the proliferation of Hut102 cells by activating the p38MAPK signal pathway, thus inducing apoptosis of certain Hut102 cells.

相似文献/References:

[1]程晓明,王长征,钱桂生,等.雷公藤对致敏小鼠T细胞活化及其IL-5 mRNA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2002,24(02):0.[doi:10.16016/j.1000-5404.2002.02.028 ]
 CHENG Xiao ming,WANG Chang zheng,QIAN Gui sheng.[J].J Amry Med Univ (J Third Mil Med Univ),2002,24(19):0.[doi:10.16016/j.1000-5404.2002.02.028 ]
[2]杨彦春,杨军,刘韬,等.蕈样肉芽肿累及腭部1例[J].陆军军医大学学报(原第三军医大学学报),2003,25(09):0.[doi:10.16016/j.1000-5404.2003.09.044 ]

更新日期/Last Update: 2010-10-08