[1]苏保,蒋电明,严永刚,等.氨基酸聚合物/硫酸钙生物工程骨的生物相容性研究[J].第三军医大学学报,2011,33(01):40-44.
 Su Bao,Jiang Dianming,Yan Yonggang,et al.Study on biocompatibility of poly(amino acid)/calcium sulfate composite as bioengineering bone[J].J Third Mil Med Univ,2011,33(01):40-44.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
33卷
期数:
2011年第01期
页码:
40-44
栏目:
论著
出版日期:
2011-01-15

文章信息/Info

Title:
Study on biocompatibility of poly(amino acid)/calcium sulfate composite as bioengineering bone
作者:
苏保蒋电明严永刚赵增辉李军
重庆医科大学附属第一医院骨科;四川大学物理科学与技术学院
Author(s):
Su Bao Jiang Dianming Yan Yonggang Zhao Zenghui  Li Jun
Department of Orthopaedics,  First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016,College of Physical Science and Technology, Sichuan University, Chengdu, Sichuan Province, 610064, China
关键词:
氨基酸聚合物硫酸钙生物相容性可降解
Keywords:
poly(amino acid)calcium sulfatebiocompatibilitybiodegradable
分类号:
R318.08;R322.71
文献标志码:
A
摘要:
目的  评价氨基酸聚合物[poly(amino acid),PAA]/硫酸钙(calcium sulfate,CaSO4)生物工程骨的生物相容性。  方法   制备PAA/CaSO4。通过急性毒性实验、溶血实验、微核实验、热原实验、皮内刺激实验、细胞毒性实验研究该复合材料的生物相容性。  结果   急性毒性实验中各组小鼠活动正常,7 d内小鼠无死亡,各组动物未见中毒症状或不良反应,7 d 后各组小鼠体质量日平均增加差异无统计学意义(P>0.05)。材料浸提液的溶血率为2.01%,符合<5%的国家标准。微核实验中3个实验组与阴性对照组微核率无明显差异(各组的微核率均介于0.4~0.6之间),材料无潜在致突变性。热原实验中3只动物中体温升高最高为0.4℃,体温升高的总数为1.1℃,符合热源实验国家标准。皮内刺激实验观察显示,注射材料生理盐水浸提液和生理盐水处无明显红斑、水肿和皮肤坏死,PII 为0.01~0.02,属极轻微刺激。细胞毒性实验中A、B、C组的BMSC的相对增殖率均>90%,细胞毒性为0~1级,材料无细胞毒性。  结论   PAA/CaSO4具有良好的生物相容性。
Abstract:
Objective  To evaluate the biocompatibility of poly(amino acid)/calcium sulfate (PAA/CaSO4) composite as bioengineering bone.   Methods   The PAA in the composite consists of 6-aminohexanoic acid, glycine, alanine, phenylalanine, proline, lysine, and hydroxyproline. Physiological saline lixivium of the composite was used in acute toxicity test, hemolysis test, micronucleus test, intradermal irritation test, pyrogen test, and cytotoxicity test to evaluate the biocompatibility of the material.   Results   The activity of mice was normal in the acute toxicity test. No animal died and no toxicity symptom or adverse effect was shown within 7 d. The average daily weight increases showed no statistically significant difference (P>0.05) between the experimental and control groups after 7 d. The hemolysis rate of the lixivium was 2.01%, which was lower than the national standard (<5%). The micronucleus rates showed no statistically significant difference (P>0.05) between three experimental groups and the negative control group (each group’s micronucleus rate was between 0.4 and 0.6), and potential mutagenicity was not observed in the micronucleus test. The maximum increase of body temperature in the experimental group was 0.4 ℃, and the total body temperature increase of the three experimental animals was 1.1 ℃, which met the national standard (<1.4 ℃). Intradermal irritation test results showed no obvious erythema, edema, or skin necrosis in the experimental and negative control groups, with a primary irritation index of 0.01-0.02, indicating very slight irritation. In the cytotoxicity test, the relative growth rates of two experimental groups and the negative control group were all above 90%, and the toxicity of PAA/CaSO4 composite lixivium was graded 0 or 1, indicating no cytotoxicity.   Conclusion  PAA/CaSO4 composite has good biocompatibility.

参考文献/References:

苏保, 蒋电明, 严永刚, 等. 氨基酸聚合物/硫酸钙生物工程骨的生物相容性研究[J].第三军医大学学报,2011,33(1):40-44.

更新日期/Last Update: 2011-01-11