[1]刘明明,秦伟,谢汉平,等.大鼠视皮层硫化软骨素粘多糖降解对闪光诱发电位的影响[J].第三军医大学学报,2010,32(10):1031-1033.
 Liu Mingming,Qin Wei,Xie Hanping,et al.Effect of CSPGs degradation on flash visual evoked potential in rat visual cortex[J].J Third Mil Med Univ,2010,32(10):1031-1033.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第10期
页码:
1031-1033
栏目:
论著
出版日期:
2010-05-30

文章信息/Info

Title:
Effect of CSPGs degradation on flash visual evoked potential in rat visual cortex
作者:
刘明明秦伟谢汉平余延基
第三军医大学西南医院全军眼科中心
Author(s):
Liu Mingming Qin Wei Xie Hanping Yu Yanji
Cneter of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
关键词:
硫化软骨素粘多糖闪光视觉诱发电位视皮层视觉发育
Keywords:
chondroitin sulfate proteoglycans flash visual evoked potential visual cortex visual development
分类号:
R338.25;R338.8;R339.14
文献标志码:
A
摘要:
目的   在视觉发育可塑性关键期终止前后,观察软骨素酶ABC(chondroitinase ABC, ChABC)降解Long Evans 大鼠视皮层硫化软骨素粘多糖(chondroitin sulfate proteoglycans, CSPGs)后闪光视觉诱发电位 (flash visual evoked potential, F-VEP)的变化。   方法   对出生后21、35、49 d正常组及同时间点ChABC酶视皮层灌注组大鼠进行F-VEP检查。   结果   正常组大鼠21、35、49 d,F-VEP主波的潜时逐渐缩短,分别为[(63.5±10.1),(50.8±6.4),(44.9±6.3) ms,P<0.05],波峰值由21 d的(15.7±3.8) ms增加至35 d的(26.6±4.0) ms (P<0.05), 49 d较35 d稍降低。35、49 d ChABC酶视皮层灌注组大鼠F-VEP主波的潜时分别为(62.5±8.0),(58.4±7.6)ms,较同时间点正常组明显延长(P<0.05),主波振幅两组间各时间点无明显差异(P>0.05)。   结论   在视觉发育可塑性关键期终止前后,降解大鼠视皮层CSPGs影响了F-VEP的发育变化。
Abstract:
Objective   To investigate the effects of chondroitin sulfate proteoglycans (CSPGs) degradation induced by chondroitinase ABC (ChABC) on the rat flash visual evoked potential (F-VEP) around the end of the critical period of visual development.    Methods   A total of 30 Long Evans rats were randomly divided into 3 groups according to their age of 14, 21 and 35 d. Then in each group, 5 animals served as CSPGs degradation model induced by ChABC microinjection into 5 sites of the rat cortex [750 nL (48 U/ml) for 1 site, for 2 times at a 3-day interval], and the other 5 rats served as normal control and received normal saline microinjection. Immunofluorescence microscopy and laser scanning confocal microscopy were used to determine the establishment of animal model. F-VEPs were evaluated in 7 d after first microinjection.    Results   In the normal rats, the latency of F-VEP main wave became shorter with the increase of their age, the number was [(63.5±10.1),(50.8±6.4),(44.9±6.3) ms,P<0.05], whereas the amplitude of F-VEP main wave was first increased from (15.7±3.8) ms to (26.6±4.0) ms at early postnatal days (P<0.05) and decreased slightly at postnatal day 49. In the ChABC treated rats, the latency of F-VEP main wave became longer than that of normal rat on postnatal day 35 and 49, the number was (62.5±8.0),(58.4±7.6)ms  (P<0.05). But there was no obvious change of the F-VEP amplitude between the normal rats and ChABC treated rats.    Conclusion   Around the end of the critical period of visual development, CSPGs degradation has an effect on the development of rat F-VEPs.

参考文献/References:

刘明明, 秦伟, 谢汉平, 等. 大鼠视皮层硫化软骨素粘多糖降解对闪光诱发电位的影响[J].第三军医大学学报,2010,32(10):1031-1033.

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更新日期/Last Update: 2010-05-20