[1]赵宸,杨宗林,司维柯,等.Wnt5a基因在急性髓细胞白血病病例中的表达分析[J].第三军医大学学报,2010,32(10):1034-1037.
 Zhao Chen,Yang Zonglin,Si Weike,et al.Expression of Wnt5a gene in myeloblastic leukemia[J].J Third Mil Med Univ,2010,32(10):1034-1037.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第10期
页码:
1034-1037
栏目:
论著
出版日期:
2010-05-30

文章信息/Info

Title:
Expression of Wnt5a gene in myeloblastic leukemia
作者:
赵宸杨宗林司维柯邓刚牛长春袁媛李招权潘静
第三军医大学医学检验系临床血液学教研室
Author(s):
Zhao Chen Yang Zonglin Si Weike Deng Gang Niu Changchun Yuan Yuan Li Zhaoquan Pan Jing
Department of Clinical Hematology, College of Medical Laboratory, Third Military Medical University, Chongqing, 400038, China
关键词:
Wnt5a基因表达急性髓细胞白血病血液病
Keywords:
Wnt5a acute myeloblastic leukemia gene expression
分类号:
R733.71;R730.43;R394.2
文献标志码:
A
摘要:
目的   检测Wnt5a基因在良性血液病、急性髓细胞白血病及缓解病例骨髓中的表达情况,探讨Wnt5a在急性髓细胞白血病发生中的作用。   方法   RT-PCR方法检测18例良性血液病病例,30例急性髓细胞白血病初诊病例和25例缓解病例骨髓中Wnt5a mRNA的表达情况;免疫细胞化学法检测对应骨髓中Wnt5a蛋白的表达情况。   结果   Wnt5a mRNA在良性血液病、急性髓细胞白血病病例和缓解病例中的阳性表达率分别为88.9%(16/18)、30%(9/30)和68.0%(17/25),半定量值分别为(0.382 6±0.190 8)、(0.104 1±0.059 0)和(0.260 6±0.146 6)。统计学分析Wnt5a mRNA在初诊病例中的表达明显低于缓解病例(P<0.05),缓解病例与良性血液病之间无明显差异(P>0.05);Wnt5a蛋白在良性血液病中阳性表达,在急性髓细胞白血病初诊病例中低表达或表达缺失,在缓解病例中表达恢复。   结论   Wnt5a基因在急性髓细胞白血病初诊病例中表达缺失而在治疗缓解病例中表达恢复,提示急性髓系白血病的发生可能与Wnt5a 基因表达缺失或下调有关,Wnt5a可以作为一个髓系白血病诊断、指导治疗和疗效评价的指标。
Abstract:
Objective   To detect the expression of Wnt5a gene in the bone marrow of benign hematologic diseases, acute myeloblastic leukemia, and in complete remission (CR), and investigate the role of Wnt5a in the pathogenesis of myeloblastic leukemia.    Methods   The expression of Wnt5a gene was detected with semiquantitative RT-PCR in 18 cases of benign hematologic disease, 30 cases of acute myeloblastic leukemia (AML) and 25 cases of acute myeloblastic leukemia who were in CR. The expression of Wnt5a protein was analyzed by immunocytochemical staining in some benign, AML and CR samples.    Results   The positive rate and expression level of Wnt5a mRNA, respectively, were 30% and (0.104 1±0.059 0) for AML patients, which are lower than 68.0% and (0.260 6±0.146 6) for CR patients (P<0.05), and also 88.9% and (0.382 6±0.190 8) in benign diseases. Wnt5a protein was decreased or missed in AML patients, but it was positively expressed in CR cases and benign cases.    Conclusion   The expression of Wnt5a gene is decreased in AML patients but restored in AML-CR cases, and there is no statistical difference between benign and CR. Our results indicate that AML may be correlated with down-regulation or loss of Wnt5a expression. Wnt5a gene might be used as a marker for diagnosis and target of therapy in myeloblastic leukemia.

参考文献/References:

赵宸, 杨宗林, 司维柯, 等. Wnt5a基因在急性髓细胞白血病病例中的表达分析[J].第三军医大学学报,2010,32(10):1034-1037.

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更新日期/Last Update: 2010-05-20