[1]乔平云,周江堡,徐晓晓,等.脑源性神经营养因子对皮层神经细胞的保护作用及其机制[J].第三军医大学学报,2010,32(14):1504-1507.
 Qiao Pingyun,Zhou Jiangbao,Xu Xiaoxiao,et al.Protective effect of brain-derived neurotrophic factor on high dose glutamate-injured rat cortical neurons and its mechanism[J].J Third Mil Med Univ,2010,32(14):1504-1507.
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脑源性神经营养因子对皮层神经细胞的保护作用及其机制(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第14期
页码:
1504-1507
栏目:
论著
出版日期:
2010-07-30

文章信息/Info

Title:
Protective effect of brain-derived neurotrophic factor on high dose glutamate-injured rat cortical neurons and its mechanism
作者:
乔平云周江堡徐晓晓吴鹏张会春
重庆医科大学附属儿童医院神经内科
Author(s):
Qiao Pingyun Zhou Jiangbao Xu Xiaoxiao Wu Peng Zhang Huichun
Department of Neurology, Children’s Hospital, Chongqing Medical University, Chongqing, 400014, China
关键词:
脑源性神经营养因子皮层神经元谷氨酸神经毒性p75NTRJNKERK
Keywords:
brain-derived neurotrophic factor cortical neurons glutamate neurotoxicity p75NTR JNK ERK
分类号:
R742.02;R965;R977
文献标志码:
A
摘要:
目的    探讨脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)对高浓度谷氨酸损伤皮层神经元的保护作用及机制。    方法    原代培养皮层神经元细胞,通过建立谷氨酸组、BDNF组、对照组进行对照研究。AO/EB荧光染色法观察细胞凋亡形态,MTT法观察细胞凋亡率,Western  blot观察p75NTR、JNK、ERK等蛋白的表达量。    结果    与空白对照组(1.26±0.06)相比,谷氨酸组细胞活力(0.72±0.10)显著降低(P<0.05);而与谷氨酸组比较,BDNF组(1.14±0.06)细胞活力显著提高(P<0.01)。谷氨酸组细胞凋亡比BDNF组明显增多(P<0.05)。荧光染色谷氨酸组细胞膜破坏增多。Western  blot检测结果示:p75NTR表达在BDNF组(0.78±0.09)和谷氨酸组(0.90±0.18)均高于对照组(0.15±0.12)(P<0.05);JNK在BDNF组(0.56±0.16)比谷氨酸组(0.95±0.06)表达显著降低(P<0.05);而ERK在BDNF组(0.83±0.16)比谷氨酸组(0.57±0.08)表达显著升高(P<0.05),ERK在谷氨酸组中显著降低。    结论    BDNF对皮层神经元细胞具有保护作用,主要通过调节p75NTR和TrkB受体之间的比例,上调ERK,下调JNK蛋白的表达来抑制凋亡,增加细胞的存活。
Abstract:
Objective    To explore the protective effect of brain-derived neurotrophic factor (BDNF) on cultured rat cortical neurons against glutamate (Glu)-induced injury and its mechanism.     Methods    Cortical neurons were primarily cultured from 1-day-old newborn Sprague-Dawley rats and then cultured for 7 d. The cortical neurons were divided randomly into 3 groups: control group, Glu group and BDNF group after identified with neuron-specific enolase (NSE) immunostaining. The cells of BDNF were treated with 50 ng/ml BDNF on day 6 for 24 h followed by cultured with 50 μmol/L Glu for 0.5 h. While, the cells of Glu group were cultured with 50 μmol/L Glu for 0.5 h on day 7. The control cells received no such treatments. On day 8, cell viability were determined by the colorimetric MTT assay. The morphological features of the neuron cells were observed under AO/EB fluorescence microscopy. Expressions of p75NTR, JNK and ERK were observed using Western blot analysis.     Results    On day 8, the primary cortical neurons grew well. BDNF protected cortical neural cells from Glu injury. Cell viability of BDNF group was (1.14±0.06), significantly higher than that of Glu group (0.72±0.10, P<0.01), though which was lower than that of the control group (1.26±0.06). More cell apoptosis was observed in Glu group than in BDNF group (P<0.05). The expression of p75NTR was higher in Glu group (0.90±0.18) and BDNF group (0.78±0.09) than in normal control group (0.15±0.12, P<0.05); the expression of JNK was lower in the BDNF group (0.56±0.16) than in the Glu group (0.95±0.06, P<0.05); at the same time, the expression of ERK was opposite to that of JNK (0.83±0.16 in the BDNF group vs 0.57±0.08 in the Glu group, P<0.05).     Conclusion    BDNF protects cultured rat cortical neurons from the injury induced by high-dose Glu, which may be through changing the proportion between p75NTR and TrkB, up-regulating ERK and down-regulating JNK to suppress cell apoptosis and improve cell viability.

参考文献/References:

乔平云,周江堡,徐晓晓,等. 脑源性神经营养因子对皮层神经细胞的保护作用及其机制[J].第三军医大学学报,2010,32(14):1504-1507.

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更新日期/Last Update: 2010-07-19