[1]齐志国,朱晓峰,富奇志,等.神经干细胞与血管内皮祖细胞共移植促进移入缺血再灌注模型大鼠脑内神经干细胞向神经元分化和突触的形成[J].陆军军医大学学报(原第三军医大学学报),2010,32(07):625-629.
　Qi Zhiguo,Zhu Xiaofeng,Fu Qizhi,et al.Complex of neural stem cells and endothelial progenitor cells enhances differentiation of neural stem cells to neuron and formation of synapse after implanted in MCAO rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(07):625-629.
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神经干细胞与血管内皮祖细胞共移植促进移入缺血再灌注模型大鼠脑内神经干细胞向神经元分化和突触的形成(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
32卷
期数:
2010年第07期
页码:
625-629
栏目:
论著
出版日期:
2010-04-15

文章信息/Info

Title:
Complex of neural stem cells and endothelial progenitor cells enhances differentiation of neural stem cells to neuron and formation of synapse after implanted in MCAO rats
作者:
齐志国; 朱晓峰; 富奇志; 谢鹏
重庆医科大学：附属第一医院神经内科,神经科学中心;佳木斯大学
Author(s):
Qi Zhiguo;  Zhu Xiaofeng;  Fu Qizhi;  Xie Peng
Department of Neurology, First Affilitated Hospital, Neurosciences Research Center, Chongqing Medical University, Chongqing, 400016； Jamusi University, Jiamusi, Heilongjiang Province, 154002, China
关键词:
神经干细胞; 内皮祖细胞; 缺血再灌注; 分化; 突触
Keywords:
neuron stem cells;  endothelial progenitor cells;  ischemic and reperfusion;  differentiation;  synapse
分类号:
R743.31;R329.2;R-33
文献标志码:
A
摘要:
目的  研究神经干细胞与血管内皮祖细胞构建的复合移植体对移植入缺血再灌注模型大鼠缺血半暗区的神经干细胞向神经元分化及突触形成的影响。  方法  300只SD大鼠根据完全随机原则分为假手术组、缺血对照组、缺血神经干细胞移植组、缺血血管内皮祖细胞移植组和缺血共移植复合体移植组。分离培养新生大鼠海马神经干细胞和外周血管内皮祖细胞，利用神经干细胞和血管内皮祖细胞及层粘连蛋白共建共移植复合体；将神经干细胞、血管内皮祖细胞及共移植复合体分别移入缺血再灌注模型大鼠缺血半暗区，在1、3、7、14、30、60 d，利用免疫荧光双标观察神经干细胞向神经元分化情况；免疫组化观察突触素P38在缺血半暗区的表达，RT-PCR检测突触蛋白-Ⅰ和连接蛋白43mRNA在缺血半暗区表达，Western blot法分析突触小泡蛋白在缺血半暗区表达。  结果  与缺血神经干细胞移植组相比，缺血共移植复合体移植组除1 d外，其余各时间点Brdu-神经元特异性烯醇化酶双阳性细胞数增加（P<0.05, P<0.01）；突触蛋白-Ⅰ和连接蛋白43mRNA表达增加（P<0.05, P<0.01）；突触蛋白-Ⅰ蛋白表达增加（P<0.01, P<0.05）；除1、3 d外，其余各时间点突触素P38免疫阳性反应产物吸光度值增大（P<0.05, P<0.01）。  结论  神经干细胞和血管内皮祖细胞共移植，可促进移入大鼠缺血半暗区神经干细胞向神经元分化，并促进其突触形成和神经网络的重建。
Abstract:
Objective  To explore the effect of the complex containing neuron stem cells (NSCs) and endothelial progenitor cells (EPCs) after being transplanted to ischemia and reperfusion rat model on the differentiation of neural stem cells to neuron and synapse formation.   Methods  Totally 300 SD adult male rats were randomly divided into sham operation group, middle cerebral artery occlusion (MACO) model group, MACO+NSCs group, MACO+EPCs group, and MACO+complex group, and every group were further divided into 6 subgroups according to time points 1, 3, 7, 14, 30 and 60 d after operation, NSCs were isolated from the hippocampus of neonatal SD rats born in 24 h and identified with Nestin staining. EPCs were obtained from the artery blood of SD rats and verified with immunocytochemical stainings of CD31 and CD34 after culture. NSCs and EPCs were cultured together to produce the complex with aid of laminin. Then normal saline, the NSCs, EPCs, or complex (2×1010/L) were transplanted into the ischemia penumbra of corresponding model rat brain,  sham control or MACO rats. The differentiation of NSCs, the expression of P38, synapsin-I, mRNA of connexin 43 were observed with double label immunofluorescence technique, immunohistochemical method, RT-PCR and Western blot analysis in ischemic penumbra.   Results  Compared with other groups, there were more double Brdu and neural special enolase positive cells in MACO+complex group with the increased expression of P38. RT-PCR and Western blot analysis indicated that MACO+complex group had significantly higher expressions of synapsin-I at mRNA and protein levels in ischemic penumbra, and so did the mRNA expression of connexin 43.   Conclusion  The transplantation of the complex of NSCs and EPCs improves the NSCs differentiation to neuron, synapse formation and reconstruction of neural network.

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更新日期/Last Update: 2010-04-02