[1]洪毅,蹇朝,肖颖彬.自噬体在紫绀型先天性心脏病患儿心肌中的表达[J].第三军医大学学报,2009,31(23):2351-2354.
 HONG Yi,JIAN Zhao,XIAO Ying-bin.Upregulated autophagy in myocardium of infants with cyanotic congenital heart defects[J].J Third Mil Med Univ,2009,31(23):2351-2354.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
31卷
期数:
2009年第23期
页码:
2351-2354
栏目:
论著
出版日期:
2009-12-15

文章信息/Info

Title:
Upregulated autophagy in myocardium of infants with cyanotic congenital heart defects
作者:
洪毅蹇朝肖颖彬
第三军医大学新桥医院全军心血管外科中心
Author(s):
HONG Yi JIAN Zhao XIAO Ying-bin
Department of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
紫绀型先天性心脏病自噬体LC3-Ⅱ心肌慢性缺氧适应
Keywords:
cyanotic congenital heart defects autophagosome microtubule-associated protein 1 light chain3 (LC-3) myocardial adaptation chronic hypoxia
分类号:
R322.11;R329.25;R725.411
文献标志码:
A
摘要:
目的    检测紫绀型先天性心脏病患儿心肌细胞中自噬体的表达,探讨细胞自噬作用在心肌慢性缺氧适应中的意义。    方法    入选行手术矫治的先天性心脏病患儿18例,其中紫绀型先天性心脏病患儿10例,非紫绀型先天性心脏病患儿8例。取手术中切除的右室流出道心肌组织作为标本,采用透射电镜技术观察心肌细胞超微结构的改变,应用Western blot检测自噬体特异性标志物LC3-Ⅱ的表达情况。     结果    紫绀组患儿术前血氧饱和度明显低于非紫绀组(P<0.01);紫绀组患儿心肌细线粒体数量增多、排列紊乱,内质网肿胀,可见典型自噬体形成;与非紫绀组相比,紫绀组患儿LC3-Ⅱ蛋白表达显著增高(P<0.01)。     结论    紫绀型先天性心脏病患儿心肌细胞自噬作用增强,可能参与慢性缺氧条件下心肌细胞的适应性调节。
Abstract:
Objective    In the heart, autophagy is important for the turnover of organelles at low basal levels under normal conditions, but is upregulated in response to stresses such as ischemia/reperfusion and in cardiovascular diseases such as heart failure. It can prevent the heart from injury under stress conditions. This study was designed to test the hypothesis that autophagy would be upregulated in the myocardium of children with cyanotic congenital cardiac defects.     Methods    Eighteen children with cyanotic (n=10) or acyanotic cardiac defects (n=8) who were admitted in our hospital from October 2008 to April 2009 and received surgical treatment were investigated. Samples from the right ventricular myocardium taken immediately after aortic clamping were morphologically studied with transmission electron microscopy for the ultrastructure. Microtubule-associated protein 1 light chain 3 (LC-3) was detected by Western blot analysis in the obtained samples.     Results    Children with cyanotic cardiac defects had higher oxyhemoglobin saturation before operation than those with acyanotic cardiac defects. Electron microscopy showed that the former group had more mitochondria, disordered arrangement, swollen endoplasmic reticulum and typical autophagosomes in the myocardial cytosol. LC3-II were significantly elevated  in patients with cyanotic compared with those with acyanotic congenital cardiac defects (P<0.01).     Conclusion    Chronic hypoxemia is associated with the induction of autophagy. Thus, up-regulated autophagy might particularly participate in myocardial adaption to chronic hypoxia in children with cyanotic congenital cardiac defects.

参考文献/References:

洪毅,蹇朝,肖颖彬.自噬体在紫绀型先天性心脏病患儿心肌中的表达[J]. 第三军医大学学报,2009,31(23):2351-2354.

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[2]左松,高云华,杨成业,等.紫绀型先天性心脏病的超声诊断价值[J].第三军医大学学报,2001,23(04):0.[doi:10.16016/j.1000-5404.2001.04.037 ]
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更新日期/Last Update: 2009-11-23